The role of the gas phase in the greening and growth of illuminated cell suspension cultures of spinach (Spinacia oleracea, L.)

In Vitro ◽  
1976 ◽  
Vol 12 (7) ◽  
pp. 485-494 ◽  
Author(s):  
C. C. Dalton ◽  
H. E. Street
Keyword(s):  
Cell Suspension ◽  
Gas Phase ◽  
Spinacia Oleracea ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Spinacia Oleracea L

Biosynthese von Cyanidin in Zellsuspensionskulturen von Haplopappus gracilis / Biosynthesis of Cyanidin in Cell Suspension Cultures of Haplopappus gracilis

1971 ◽  
Vol 26 (6) ◽  
pp. 581-585 ◽  
Author(s):  
Hansjörg Fritsch ◽  
Klaus Hahlbrock ◽  
Hans Grisebach
Keyword(s):  
Cell Suspension ◽  
Phenylalanine Ammonia Lyase ◽  
Cell Suspension Cultures ◽  
Maximal Activity ◽  
Suspension Cultures ◽  
Feeding Time ◽  
Haplopappus Gracilis ◽  
Time Required ◽  
Anthocyanin Production

Earlier work with buckwheat seedlings showed that dihydrokaempferol (3,5,7,4′-tetrahydroxyflavanone) (1) is a good precursor for cyanidin and quercetin. Since cell suspension cultures have several advantages for the quantitative comparison of the incorporation of a radioactive precursor into a product, the role of 1 in the biosynthesis of cyanidin was reinvestigated with cell cultures of Haplopappus gracilis. These cultures show good production of cyanidin-glycosides in blue light in a medium containing sucrose, 2,4-D and Fe-EDTA. Maximum anthocyanin production and maximal activity of phenylalanine ammonia-lyase is reached about 60 — 70 hrs. after the onset of illumination. The incorporation of 1 and of phenylalanine and 4,2′,4′,6′-tetrahydroxychalcone into cyanidin was compared under identical conditions after 1, 3 and 6 hrs. feeding time. 1 prooved to be by far the best precursor for cyanidin. The feeding time required was much shorter and the incorporation rates were much higher than in experiments with seedlings.

Role of reactive oxygen species and proline cycle in anthraquinone accumulation in Rubia tinctorum cell suspension cultures subjected to methyl jasmonate elicitation

2011 ◽  
Vol 49 (7) ◽  
pp. 758-763 ◽  
Author(s):  
María Perassolo ◽  
Carla Verónica Quevedo ◽  
Víctor Daniel Busto ◽  
Ana María Giulietti ◽  
Julián Rodríguez Talou
Keyword(s):  
Reactive Oxygen Species ◽  
Methyl Jasmonate ◽  
Cell Suspension ◽  
Reactive Oxygen ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Oxygen Species ◽  
Rubia Tinctorum

The role of ethylene in berberine production byThalictrum minus cell suspension cultures

1991 ◽  
Vol 30 (11) ◽  
pp. 3605-3609 ◽  
Author(s):  
Yoshinori Kobayashi ◽  
Masakazu Hara ◽  
Hiroshi Fukui ◽  
Mamoru Tabata
Keyword(s):  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Suspension Cultures

The Role of Lipid Peroxidation in Aluminium Toxicity in Soybean Cell Suspension Cultures

2000 ◽  
Vol 55 (11-12) ◽  
pp. 957-964 ◽  
Author(s):  
Ingo Rath ◽  
Wolfgang Barz
Keyword(s):  
Lipid Peroxidation ◽  
Cell Viability ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Aluminium Toxicity ◽  
Suspension Cultures ◽  
Al Toxicity ◽  
Primary Reactions ◽  
Glycine Max L

The primary reactions leading to Al toxicity in plant cells have not yet been elucidated. We used soybean ( Glycine max [L.] Merr.) cell suspension cultures to address the question whether lipid peroxidation plays an important role in Al toxicity. Upon transfer to an Alcontaining culture medium with a calculated Al3+ activity of 15 μᴍ soybean cells showed a distinct and longtime increase in lipid peroxidation within 4h. At the same time a drastic loss of cell viability was observed. Butylated hydroxyanisole (BHA) and N,N′-diphenyl-pphenylenediamine (DPPD), two lipophilic antioxidants, were able to almost completely suppress lipid peroxidation in Al-treated cells at a concentration of 20 μᴍ. This effect was dosedependent for DPPD and was observed at minimum concentrations of 1 -2 μᴍ. When lipid peroxidation was suppressed by DPPD or BHA cell viability remained high even in the presence of toxic Al concentrations. These results suggest that Al-induced enhancement of lipid peroxidation is a decisive factor for Al toxicity in suspension cultured soybean cells.

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