Nucleosomal structure as probed by H3 histone thiol reactivity

1987 ◽  
Vol 10 (1) ◽  
pp. 1-13 ◽  
Author(s):  
Nicoletta Ferrari ◽  
Ulrich Pfeffer ◽  
Giorgio Vidali
Keyword(s):  
Thiol Reactivity ◽  
Nucleosomal Structure

scholarly journals Reaction of Chalcones with Cellular Thiols. The Effect of the 4-Substitution of Chalcones and Protonation State of the Thiols on the Addition Process. Diastereoselective Thiol Addition

Molecules ◽  
2021 ◽  
Vol 26 (14) ◽  
pp. 4332
Author(s):  
Fatemeh Kenari ◽  
Szilárd Molnár ◽  
Pál Perjési
Keyword(s):  
Biological Effects ◽  
Equilibrium Composition ◽  
Deprotonated Form ◽  
Degree Of Ionization ◽  
Thiol Reactivity ◽  
Michael Type Addition ◽  
Ph Conditions ◽  
The Difference ◽  
Initial Reactivity

Several biological effects of chalcones have been reported to be associated with their thiol reactivity. In vivo, the reactions can result in the formation of small-molecule or protein thiol adducts. Both types of reactions can play a role in the biological effects of this class of compounds. Progress of the reaction of 4-methyl- and 4-methoxychalcone with glutathione and N-acetylcysteine was studied by the HPLC-UV-VIS method. The reactions were conducted under three different pH conditions. HPLC-MS measurements confirmed the structure of the formed adducts. The chalcones reacted with both thiols under all incubation conditions. The initial rate and composition of the equilibrium mixtures depended on the ratio of the deprotonated form of the thiols. In the reaction of 4-methoxychalcone with N-acetylcysteine under strongly basic conditions, transformation of the kinetic adduct into the thermodynamically more stable one was observed. Addition of S-protonated N-acetylcysteine onto the polar double bonds of the chalcones showed different degrees of diastereoselectivity. Both chalcones showed a Michael-type addition reaction with the ionized and non-ionized forms of the investigated thiols. The initial reactivity of the chalcones and the equilibrium composition of the incubates showed a positive correlation with the degree of ionization of the thiols. Conversions showed systematic differences under each set of conditions. The observed differences can hint at the difference in reported biological actions of 4-methyl- and 4-methoxy-substituted chalcones.

scholarly journals Innenrücktitelbild: Cyclometalated Gold(III)‐Hydride Complexes Exhibit Visible Light‐Induced Thiol Reactivity and Act as Potent Photo‐Activated Anti‐Cancer Agents (Angew. Chem. 27/2020)

2020 ◽  
Vol 132 (27) ◽  
pp. 11252-11252
Author(s):  
Hejiang Luo ◽  
Bei Cao ◽  
Albert S. C. Chan ◽  
Raymond Wai‐Yin Sun ◽  
Taotao Zou
Keyword(s):  
Visible Light ◽  
Hydride Complexes ◽  
Thiol Reactivity ◽  
Anti Cancer

scholarly journals Variant Chromatin Structure of theoriP Region of Epstein-Barr Virus and Regulation of EBER1 Expression by Upstream Sequences andoriP

2001 ◽  
Vol 75 (13) ◽  
pp. 6235-6241 ◽  
Author(s):  
Barbara Wensing ◽  
Albert Stühler ◽  
Peter Jenkins ◽  
Martine Hollyoake ◽  
Claudio Elgueta Karstegl ◽  
...  
Keyword(s):  
Epstein Barr Virus ◽  
Virus Genome ◽  
Micrococcal Nuclease ◽  
Matrix Attachment Region ◽  
Barr Virus ◽  
Latently Infected ◽  
Attachment Region ◽  
Nuclear Matrix Attachment Region ◽  
Epstein Barr ◽  
Nucleosomal Structure

ABSTRACT Most of the Epstein-Barr virus genome in latently infected cells is in a standard nucleosomal structure, but the region encompassingoriP and the Epstein-Barr virus-encoded small RNA (EBER) genes shows a distinctive pattern when digested with micrococcal nuclease. This pattern corresponds to a previously mapped nuclear matrix attachment region. Although the EBER genes are adjacent to oriP, there is only a two- to fourfold effect oforiP on EBER expression. However, sequences containing a consensus ATF site upstream of EBER1 are important for EBER1 expression.

scholarly journals A DNA fragment containing the upstream activator sequence determines nucleosome positioning of the transcriptionally repressed PHO5 gene of Saccharomyces cerevisiae.

1986 ◽  
Vol 6 (7) ◽  
pp. 2298-2304 ◽  
Author(s):  
L W Bergman
Keyword(s):  
Transcriptional Regulation ◽  
Nucleosome Positioning ◽  
Vector System ◽  
Growth Media ◽  
Flanking Sequences ◽  
Dna Fragment ◽  
Acid Phosphatase Gene ◽  
Nucleosomal Structure ◽  
Relationship Of ◽  
Pho5 Gene

The functional relationship of nucleosome positioning and gene expression is not known. Using high-copy plasmids, containing the yeast phosphate-repressible acid phosphatase gene (PHO5) and the TRP1/ARS1 vector system, I have determined the nucleosomal structure of the 5' region of the PHO5 gene and demonstrated that the nucleosomal positioning of this region is independent of orientation or position in the various plasmid constructions utilized. However, deletion of a 278-base pair BamHI-ClaI fragment from the 5'-flanking sequences of the PHO5 gene causes the nucleosome positioning to become dependent on orientation or position in the plasmids tested. Use of PHO5-CYC1-lACZ fusions have demonstrated that this DNA fragment contains the sequences responsible for the transcriptional regulation of the PHO5 gene in response to the level of phosphate in the growth media. The nucleosome positioning in the 5' region of PHO5 may be determined by an interaction with the sequences or machinery responsible for transcriptional regulation of the gene.

scholarly journals Nucleosomal structure at hyperacetylated loci probed in nuclei by DNA-histone crosslinking

1993 ◽  
Vol 21 (20) ◽  
pp. 4734-4738 ◽  
Author(s):  
Konstantin K. Ebralidse ◽  
Tim R. Hebbes ◽  
Alison L. Clayton ◽  
Alan W. Thorne ◽  
Colyn Crane-Robinson
Keyword(s):  
Nucleosomal Structure

Identification of NLRP3 as a covalent target of 1,6-O,O-diacetylbritannilactone against neuroinflammation by quantitative thiol reactivity profiling (QTRP)

2022 ◽  
Vol 119 ◽  
pp. 105536
Author(s):  
Min-Ran Wang ◽  
Lan-Fang Huang ◽  
Cong Guo ◽  
Jing Yang ◽  
Shuai Dong ◽  
...  
Keyword(s):  
Thiol Reactivity

Thiol Reactivity in Pressure-Unfolded β-Lactoglobulin. Antioxidative Properties and Thermal Refolding

1998 ◽  
Vol 46 (2) ◽  
pp. 425-430 ◽  
Author(s):  
Rikke Ege Møller ◽  
Henrik Stapelfeldt ◽  
Leif H. Skibsted
Keyword(s):  
Antioxidative Properties ◽  
Thiol Reactivity ◽  
Β Lactoglobulin

Cyclometalated Gold(III)‐Hydride Complexes Exhibit Visible Light‐Induced Thiol Reactivity and Act as Potent Photo‐Activated Anti‐Cancer Agents

2020 ◽  
Vol 132 (27) ◽  
pp. 11139-11145
Author(s):  
Hejiang Luo ◽  
Bei Cao ◽  
Albert S. C. Chan ◽  
Raymond Wai‐Yin Sun ◽  
Taotao Zou
Keyword(s):  
Visible Light ◽  
Hydride Complexes ◽  
Thiol Reactivity ◽  
Anti Cancer
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