Study on transient expression of gus gene inChlorelia ellipsoidea (Chlorophyta) by using biolistic particle delivery system

1998 ◽  
Vol 16 (S1) ◽  
pp. 47-49 ◽  
Author(s):  
Chen Ying ◽  
Li Wen-bin ◽  
Bai Qin-hua ◽  
Sun Yong-ru
Keyword(s):  
Transient Expression ◽  
Delivery System ◽  
Gus Gene ◽  
Particle Delivery

scholarly journals Biolistic-mediated transient gene expression in shoot apical meristems of the prickly-pear (Opuntia ficus-indica)

1999 ◽  
Vol 42 (3) ◽  
pp. 299-302 ◽  
Author(s):  
Romulo Marino Llamoca-Zárate ◽  
Luiz Ferreira Aguiar Ponte ◽  
Joerg Landsmann ◽  
Francisco de Assis Paiva Campos
Keyword(s):  
Gene Expression ◽  
Transient Expression ◽  
Transient Gene Expression ◽  
Dna Delivery ◽  
Prickly Pear ◽  
Target Tissue ◽  
Gus Gene ◽  
Transformation System ◽  
Opuntia Ficus Indica ◽  
Apical Meristems

We have demonstrated the transient expression of the GUS gene in cells of the meristematic apical dome of Opuntia ficus-indica. DNA delivery into the cells was achieved using a biolistic PDS-1000He instrument from Bio-Rad Laboratories. The transforming DNA was coated in tungsten particles with diameter of 1.3 m m and the distance between the flying disk and the target tissue was 7.5cm and the shooting pressure was adjusted to 1200 psi. This is the first demonstration that the biolistic transformation system can be used to express a transgene in a member of the Cactaceae.

scholarly journals Application of sonication-assisted Agrobacterium-mediated transformation in Chenopodium rubrum L

2011 ◽  
Vol 49 (No. 6) ◽  
pp. 255-260 ◽  
Author(s):  
J.I. Flores Solís ◽  
P. Mlejnek ◽  
K. Studená ◽  
S. Procházka
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Transient Expression ◽  
Chenopodium Rubrum ◽  
Limiting Factors ◽  
Gus Gene ◽  
Efficient Tool ◽  
Factors Affecting ◽  
Agrobacterium Mediated Transformation ◽  
Glucuronidase Activity ◽  
Gene Coding

Chenopodium rubrum belongs to the plant species in which standard Agrobacterium-mediated transformation procedures remain inefficient. We demonstrate that the employment of sonication-assisted Agrobacterium-mediated transformation (SAAT) effectively enhanced transient expression of GUS gene coding for b-glucuronidase in Chenopodium rubrum. Further the results indicated that the age of seedlings is one of the limiting factors affecting the potency of Agrobacterium tumefaciens infection. Histochemical detection of b-glucuronidase activity revealed that two-days-old seedlings were much more susceptible to infection than ten-days-old ones. According to our results SAAT technology could provide an efficient tool for obtaining stable transformants when applied to two-days-old seedlings of Chenopodium rubrum.

Regulated particle‐delivery system for vacuum furnances

1977 ◽  
Vol 14 (5) ◽  
pp. 1196-1200
Author(s):  
M. Schwab ◽  
T. L. Rossow ◽  
G. E. Mattson ◽  
W. H. Parrish
Keyword(s):  
Delivery System ◽  
Particle Delivery

scholarly journals Transient expression of a reporter gene introduced by bioballistic bombardment into Racosperma mangium (Leguminosae family) tissues

1997 ◽  
Vol 20 (3) ◽  
pp. 507-510 ◽  
Author(s):  
M. Quoirin ◽  
F. Aragão ◽  
E. Rech ◽  
D.E. De Oliveira
Keyword(s):  
Transient Expression ◽  
Reporter Gene ◽  
Acacia Mangium ◽  
Direct Transfer ◽  
Gus Gene

We report on an assay of direct transfer of DNA into calli and seeds of Racosperma (ex-Acacia) mangium, using a bioballistic method. We observed transient expression of the GUS gene in the treated tissues

Optical levitation particle delivery system for a dual beam fiber optic trap

2000 ◽  
Vol 39 (1) ◽  
pp. 26 ◽  
Author(s):  
Robert C. Gauthier ◽  
Athanasios Frangioudakis
Keyword(s):  
Delivery System ◽  
Fiber Optic ◽  
Dual Beam ◽  
Optical Levitation ◽  
Particle Delivery

Regeneration and transformation of Egyptian maize inbred lines via immature embryo culture and a biolistic particle delivery system

2003 ◽  
Vol 39 (5) ◽  
pp. 524-531 ◽  
Author(s):  
Hanaiya A. El-Itriby ◽  
Shireen K. Assem ◽  
Ebtissam H. A. Hussein ◽  
Fathy M. Abdel-Calil ◽  
Magdy A. Madkour
Keyword(s):  
Delivery System ◽  
Embryo Culture ◽  
Immature Embryo ◽  
Inbred Lines ◽  
Maize Inbred Lines ◽  
Immature Embryo Culture ◽  
Particle Delivery
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