scholarly journals Application of sonication-assisted Agrobacterium-mediated transformation in Chenopodium rubrum L

2011 ◽  
Vol 49 (No. 6) ◽  
pp. 255-260 ◽  
Author(s):  
J.I. Flores Solís ◽  
P. Mlejnek ◽  
K. Studená ◽  
S. Procházka
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Transient Expression ◽  
Chenopodium Rubrum ◽  
Limiting Factors ◽  
Gus Gene ◽  
Efficient Tool ◽  
Factors Affecting ◽  
Agrobacterium Mediated Transformation ◽  
Glucuronidase Activity ◽  
Gene Coding

Chenopodium rubrum belongs to the plant species in which standard Agrobacterium-mediated transformation procedures remain inefficient. We demonstrate that the employment of sonication-assisted Agrobacterium-mediated transformation (SAAT) effectively enhanced transient expression of GUS gene coding for b-glucuronidase in Chenopodium rubrum. Further the results indicated that the age of seedlings is one of the limiting factors affecting the potency of Agrobacterium tumefaciens infection. Histochemical detection of b-glucuronidase activity revealed that two-days-old seedlings were much more susceptible to infection than ten-days-old ones. According to our results SAAT technology could provide an efficient tool for obtaining stable transformants when applied to two-days-old seedlings of Chenopodium rubrum.

scholarly journals Agrobacterium-Mediated Stable Transformation of Medicinal Plant Andrographis paniculata Callus Expressing β-glucuronidase (GUS) Gene

2015 ◽  
Vol 18 (2) ◽  
pp. 92
Author(s):  
Erly Marwani ◽  
Agustina Tangapo ◽  
Fenny Martha Dwivany
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Gus Expression ◽  
Selective Medium ◽  
Stable Transformation ◽  
Andrographis Paniculata ◽  
Pcr Analysis ◽  
Induction Medium ◽  
Gus Gene ◽  
Glucuronidase Activity ◽  
Leaf Disks

This study was carried out to establish a stable genetic transformation in callus culture of Andrographispaniculata mediated by Agrobacterium tumefaciens. The leaf disks of A. paniculata were infected with A. tumefaciensLBA4404 carrying a binary vector pCAMBIA1304 that contain β-glucuronidase (GUS) and hygromycinphosphotransferase (hpt) genes. The infection was conducted by dipping method for one hour, followed byco-cultivation in the dark for three days. To examine transient GUS expression, the co-cultivated leaf disks wereassayed for β-glucuronidase activity and to obtain stable transformed callus, the co-cultivated leaf disks wereselected on the callus induction medium which contain 20 mg/l hygromycin for selection. The transformedcallus was periodically subcultured every three weeks into the fresh selection medium over the 15 weeksperiod. To test a stable transformation, the callus was subjected to PCR analysis for GUS gene detection. Theresults indicated that the co-cultivated leaf disks expressed GUS activity and proliferated to produce callus onthe selective medium. Analysis of PCR on the transformed callus indicated the presence 976 bp fragment thatconfi rmed the presence of β-glucuronidase gene. These fi ndings imply that the β-glucuronidase was stably integrated into A. paniculata callus culture.Keywords: Andrographis paniculata, Agrobacterium tumefaciens, andrographollide, transformed callus,β-glucuronidase gene.

scholarly journals Factors affecting Agrobacterium mediated transformation of indigenous Chlorella vulgaris Bayerinck

2018 ◽  
Vol 44 (2) ◽  
pp. 323-326
Author(s):  
Nadia Sharif ◽  
Neelma Munir ◽  
Faiza Saleem ◽  
Shugufta Naz
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Chlorella Vulgaris ◽  
Bacterial Density ◽  
Optimum Conditions ◽  
Health Food ◽  
Feed Supplement ◽  
Factors Affecting ◽  
Agrobacterium Mediated Transformation

Chlorella vulgarisis Bayerinck widely used as a health food, feed supplement, as well as in the pharmaceutical, biofuel and cosmetics industries. It has been used to determine optimum transformation conditions through Agrobacterium tumefaciens. It has been revealed that bacterial density of OD600 = 1.0, 3 days of co-cultivation at 25°C in pH 5.5, and 100 μM acetosyringone are the optimum conditions to transform C. vulgaris.

scholarly journals OPTIMIZATION OF FACTORS AFFECTING THE Agrobacterium tumefaciens- MEDIATED TRANSFORMATION OF Eucalyptus saligna

2018 ◽  
Vol 41 (3) ◽  
Author(s):  
Yohana de Oliveira-Cauduro ◽  
Lais Gomes Adamuchio ◽  
João Carlos Bespalhok Filho ◽  
Isabel Rodrigues Gerhardt ◽  
Juliana Degenhardt-Goldbach ◽  
...  
Keyword(s):  
Gene Expression ◽  
Agrobacterium Tumefaciens ◽  
Culture Medium ◽  
Culture Media ◽  
Gus Gene ◽  
Eucalyptus Saligna ◽  
Factors Affecting ◽  
Gus Gene Expression ◽  
Positive Effect ◽  
Selection Of

ABSTRACT This study aimed to evaluate the effect of factors that may affect the genetic transformation of cotiledonary explants of Eucalyptus saligna mediated by EHA105 strain of Agrobacterium tumefaciens. The vector pBI121 carrying gus gene under control of 35S CaMV promoter was used. The effect of the following factors was evaluated: explant pre-culture, use of different antibiotics and presence of acetosyringone (AS) in co-culture media. An antioxidant solution was also used during excision, containing ascorbic acid (250mg.L-1), citric acid (25mg.L-1) and PVP-40 (1g.L-1). Pre-culture of the explants before the co-culture with bacteria was done over a 4-day period in MS culture medium supplemented with 4.4µM BAP and 2.7ìM NAA. After theco-culture period, three concentrations of kanamycin (12.5;25 and 50mg.L-1) combined with 300mg.L-1 Augmentin® in the culture medium were tested The influence of the antibiotic was also evaluated by keeping the explants in a medium containing 50mg.L-1 Km and 300mg.L-1 Augmentin® or 500mg.L-1 cefotaxime. It was concluded that Augmentin® stimulates organogenesis, that a Km concentration of 12.5mg.L-1 allows selection of explants transformed with gus gene and, finally, the addition of AS (50ìM) to the liquid and solid co-culture media has a positive effect on gus gene expression. Moreover, the use of an antioxidant solution during cotyledon excision is dispensable and the pre-culture of the explants has no effect on bud regeneration or gus gene expression. A transformation efficiency of 1.5% was reached.

Organics and its Control in Reclaimed Water

2014 ◽  
Vol 507 ◽  
pp. 752-756
Author(s):  
Chao Xiang ◽  
Ping Xu ◽  
Jing Wang ◽  
Tao Wang ◽  
Ya Jun Zhang
Keyword(s):  
Water Supply ◽  
Indirect Effects ◽  
Microbial Growth ◽  
Treatment Process ◽  
Reclaimed Water ◽  
Limiting Factors ◽  
Supply Network ◽  
Water Supply Network ◽  
Factors Affecting ◽  
Comprehensive Study

Some forms of organic matter existing in the water have direct or indirect effects on microbial growth. By the investigation data over drinking water and reclaimed water, we summarized organic limiting factors that may affect the growth of microorganisms and factors affecting these water qualities in the reclaimed water supply network, such as a variety of treatment process and the residual disinfectants. Through its comprehensive study, we want to make a contribution of opinion to control the growth of microorganisms in reclaimed water supply network.

scholarly journals Transcriptomic Analysis of Arabidopsis Seedlings in Response to an Agrobacterium-Mediated Transformation Process

2018 ◽  
Vol 31 (4) ◽  
pp. 445-459 ◽  
Author(s):  
Kaixuan Duan ◽  
Christopher J. Willig ◽  
Joann R. De Tar ◽  
William G. Spollen ◽  
Zhanyuan J. Zhang
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Defense Response ◽  
Time Course ◽  
Basic Research ◽  
Defense Responses ◽  
Transformation Process ◽  
Host Responses ◽  
Nucleic Acid Metabolism ◽  
Agrobacterium Strain ◽  
Agrobacterium Mediated Transformation

Agrobacterium tumefaciens is a plant pathogen that causes crown gall disease. This pathogen is capable of transferring the T-DNA from its Ti plasmid to the host cell and, then, integrating it into the host genome. To date, this genetic transformation ability has been harnessed as the dominant technology to produce genetically modified plants for both basic research and crop biotechnological applications. However, little is known about the interaction between Agrobacterium tumefaciens and host plants, especially the host responses to Agrobacterium infection and its associated factors. We employed RNA-seq to follow the time course of gene expression in Arabidopsis seedlings infected with either an avirulent or a virulent Agrobacterium strain. Gene Ontology analysis indicated many biological processes were involved in the Agrobacterium-mediated transformation process, including hormone signaling, defense response, cellular biosynthesis, and nucleic acid metabolism. RNAseq and quantitative reverse transcription-polymerase chain reaction results indicated that expression of genes involved in host plant growth and development were repressed but those involved in defense response were induced by Agrobacterium tumefaciens. Further analysis of the responses of transgenic Arabidopsis lines constitutively expressing either the VirE2 or VirE3 protein suggested Vir proteins act to enhance plant defense responses in addition to their known roles facilitating T-DNA transformation.

Future Directions in Instrumentation, Control and Automation in the Water and Wastewater Industry

1993 ◽  
Vol 28 (11-12) ◽  
pp. 9-14 ◽  
Author(s):  
Troy D. Vassos
Keyword(s):  
Control Strategies ◽  
Treatment Plant ◽  
Dynamic Modelling ◽  
Simulation Software ◽  
Plant Performance ◽  
Limiting Factors ◽  
Time Data ◽  
Training Environment ◽  
Factors Affecting ◽  
Water And Wastewater

The need to optimize treatment plant performance and to meet increasingly stringent effluent criteria are two key factors affecting future development of instrumentation, control and automation (ICA) applications in the water and wastewater industry. Two case studies are presented which highlight the need for dynamic modelling and simulation software to assist operations staff in developing effective instrumentation control strategies, and to provide a training environment for the evaluation of such strategies. One of the limiting factors to date in realizing the potential benefits of ICA has been the inability to adequately interpret the large number of existing instrumentation inputs available at treatment facilities. The number of inputs can exceed the number of control loops by up to three orders of magnitude. The integration of dynamic modelling and expert system software is seen to facilitate the interpretation of real-time data, allowing both quantitative (instrumented) and qualitative (operator input) information to be integrated for process control. Improvements in sensor reliability and performance, and the development of biological monitoring sensors and control algorithms are also discussed.

scholarly journals Effect of Nitrogen and Boron in Seed Yield and Yield Attributing Characters of Broccoli

2015 ◽  
Vol 3 (3) ◽  
pp. 541-544
Author(s):  
A. Khanal
Keyword(s):  
Seed Yield ◽  
Crop Production ◽  
Interactive Effect ◽  
Winter Season ◽  
Limiting Factors ◽  
Plant Nutrient ◽  
Factors Affecting ◽  
Four Levels ◽  
Major Nutrients ◽  
Pod Length

Plant nutrient is one of the limiting factors affecting crop production. Nitrogen and boron are major nutrients in case of broccoli. So, an experiment was carried out to evaluate the effect of nitrogen and boron in seed yield and yield attributing characters of broccoli in Rampur, Chitwan during winter season. The experiment was laid out in factorial RCBD design with four levels of nitrogen and two levels of boron. Each plot consists of 25 plants which were separated by 60 * 60 cm spacing. There are altogether eight treatments replicates thrice. Local variety Calabrese was used.  Significant effect of different dose of nitrogen and boron on yield attributing characters was found. Also interactive effect of nitrogen and boron in number of pods, pod length, seed yield and number of seeds per pod was found significantly different.Int J Appl Sci Biotechnol, Vol 3(3): 541-544

scholarly journals INFORMATION MATERIALS ON THE PROTECTION OF DAYTIME LEPIDOPTERA (LEPIDOPTERA: HESPERIOIDEA, PAPILIONOIDEA) OF UDMURTIA: ANALYSIS OF THREATS AND LIMITING FACTORS OF SPECIES’ EXISTENCE

2019 ◽  
Vol 29 (3) ◽  
pp. 337-345
Author(s):  
D.A. Adakhovskiy
Keyword(s):  
Limiting Factors ◽  
Nature Management ◽  
Habitat Types ◽  
Factors Affecting ◽  
Population Structures ◽  
Potential Risks ◽  
Information Materials ◽  
Regional Types ◽  
Degree Of Fragmentation

The article summarizes the materials on the set of limiting criteria and factors affecting the group of threatened species of diurnal Lepidoptera of Udmurtia. The group is formed by representatives belonging to the categories "Exposed to critical danger", "Endangered" and "Vulnerable", allocated by the regional co-Zoological analysis of the fauna of Rhopalocera. A set of criteria used to identify the threatened categories includes the following indicators of limitation: arealographic (total breadth of distribution of species in the region, the degree of fragmentation of regional habitat types, trends in regional species' chorology); biotopic (quantity and quality of key habitats in the region, as well as actual and potential risks and declining trends in their quantity and quality); population (the level of localization of population structures of species). The main factors of anthropogenic impact on the habitats of moths include the regional types of nature management with landscape-scale manifestations, such as agricultural and forestry activities.

scholarly journals Toward Future Installations: Mutual Interactions of Short Intakes With Modern High Bypass Fans

2019 ◽  
Vol 141 (8) ◽  
Author(s):  
Nagabhushana Rao Vadlamani ◽  
Teng Cao ◽  
Rob Watson ◽  
Paul G. Tucker
Keyword(s):  
Pressure Fluctuations ◽  
Limiting Factors ◽  
Small Scale ◽  
Flow Distortion ◽  
Factors Affecting ◽  
Fan Performance ◽  
Shock Unsteadiness ◽  
Power Spectral ◽  
Order Of Magnitude ◽  
Underlying Mechanisms

In this paper, we investigate the coupled interaction between a new short intake design with a modern fan in a high-bypass ratio civil engine, specifically under the off-design condition of high incidence. The interaction is expected to be much more significant than that on a conventional intake. The performance of both the intake-alone and rotor-alone configurations are examined under isolation. Subsequently, a comprehensive understanding on the two-way interaction between intake and fan is presented. This includes the effect of fan on intake angles of attack (AoA) tolerance (FoI) and the effect of circumferential and radial flow distortion induced by the intake on the fan performance (IoF). In the FoI scenario, the rotor effectively redistributes the mass flow at the fan-face. The AoA tolerance of the short-intake design has increased by ≈4 deg when compared with the intake-alone configuration. Dynamic nature of distortion due to shock unsteadiness has been quantified. ST plots and power spectral density (PSD) of pressure fluctuations show the existence of a spectral gap between the shock unsteadiness and blade passing, with almost an order of magnitude difference in the corresponding frequencies. In the IoF scenario, both the “large” (O(360 deg)) and “small” scale distortion (O(10–60 deg)) induced by the intake results in a non-uniform inflow to the rotor. Sector analysis reveals a substantial variation in the local operating condition of the fan as opposed to its steady characteristic. Streamline curvature, upwash, and wake thickening are identified to be the three key factors affecting the fan performance. These underlying mechanisms are discussed in detail to provide further insights into the physical understanding of the fan-intake interaction. In addition to the shock-induced separation on the intake lip, the current study shows that shorter intakes are much more prone to the upwash effect at higher AoA. Insufficient flow straightening along the engine axis is reconfirmed to be one of the limiting factors for the short-intake design.

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