Porometric measurements indicate wound severity and tuber maturity affect the early stages of wound-healing

1995 ◽  
Vol 72 (4) ◽  
pp. 225-241 ◽  
Author(s):  
Edward C. Lulai ◽  
Paul H. Orr
Keyword(s):  
Wound Healing ◽  
Early Stages ◽  
Tuber Maturity

Neutralisation of TGF-beta 1 and TGF-beta 2 or exogenous addition of TGF-beta 3 to cutaneous rat wounds reduces scarring

1995 ◽  
Vol 108 (3) ◽  
pp. 985-1002 ◽  
Author(s):  
M. Shah ◽  
D.M. Foreman ◽  
M.W. Ferguson
Keyword(s):  
Wound Healing ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Collagen I ◽  
Tgf Beta ◽  
Early Stages ◽  
Collagen Iii ◽  
Exogenous Addition ◽  
Growth Factor Beta ◽  
Beta 2

Exogenous addition of neutralising antibody to transforming growth factor-beta 1,2 to cutaneous wounds in adult rodents reduces scarring. Three isoforms of transforming growth factor-beta (1, 2 and 3) have been identified in mammals. We investigated the isoform/isoforms of TGF-beta responsible for cutaneous scarring by: (i) reducing specific endogenous TGF-beta isoforms by exogenous injection of isoform specific neutralising antibodies; and (ii) increasing the level of specific TGF-beta isoforms by exogenous infiltration into the wound margins. Exogenous addition of neutralising antibody to TGF-beta 1 plus neutralising antibody to TGF-beta 2 reduced the monocyte and macrophage profile, neovascularisation, fibronectin, collagen III and collagen I deposition in the early stages of wound healing compared to control wounds. Treatment with neutralising antibodies to TGF-betas 1 and 2 markedly improved the architecture of the neodermis to resemble that of normal dermis and reduced scarring while the control wounds healed with scar formation. Exogenous addition of neutralising antibody to TGF-beta 1 alone also reduced the monocyte and macrophage profile, fibronectin, collagen III and collagen I deposition compared to control wounds. However, treatment with neutralising antibody to TGF-beta 1 alone only marginally reduced scarring. By contrast, wounds treated with neutralising antibody to TGF-beta 2 alone did not differ from control wounds. Interestingly, exogenous addition of the TGF-beta 3 peptide also reduced the monocyte and macrophage profile, fibronectin, collagen I and collagen III deposition in the early stages of wound healing and markedly improved the architecture of the neodermis and reduced scarring. By contrast, wounds treated with either TGF-beta 1 or with TGF-beta 2 had more extracellular matrix deposition in the early stages of wound healing but did not differ from control wounds in the final quality of scarring. This study clearly demonstrates isoform specific differences in the role of TGF-betas in wound healing and cutaneous scarring. TGF-beta 1 and TGF-beta 2 are implicated in cutaneous scarring. This study also suggests a novel therapeutic use of exogenous recombinant, TGF-beta 3 as an anti-scarring agent.

scholarly journals Nod-Like Receptor Protein-3 Inflammasome Plays an Important Role during Early Stages of Wound Healing

PLoS ONE ◽  
2015 ◽  
Vol 10 (3) ◽  
pp. e0119106 ◽  
Author(s):  
Eileen M. Weinheimer-Haus ◽  
Rita E. Mirza ◽  
Timothy J. Koh
Keyword(s):  
Wound Healing ◽  
Receptor Protein ◽  
Early Stages

Regeneration and growth control in Nereis

Development ◽  
1967 ◽  
Vol 18 (1) ◽  
pp. 67-77
Author(s):  
D. W. Golding
Keyword(s):  
Wound Healing ◽  
Intact Animal ◽  
Growth Control ◽  
Growth Zone ◽  
Anterior Border ◽  
Early Stages ◽  
Proliferation And Differentiation ◽  
Number Of Segments

The annelid body, as typified by that of Nereis, consists of a presegmental prostomium, a number of segments, and a postsegmental pygidium. The larval trochosphere is unsegmented. Once proliferation of segments has started, their number increases steadily throughout most of the life of the adrenal, though the rate of proliferation declines (Clark & Clark, 1962). New segments are added posteriorly by proliferation and differentiation of tissue comprising the growth zone, which forms the anterior border of the pygidium. After loss of the pygidium and posterior segments, wound healing is followed by the formation of a new growth zone. Amoebocyte migration (Stephan-Dubois, 1955,1956,1958) and dedifferentiation of cells adjacent to the level of transection (Herlant-Meewis & Nokin, 1962) are important in the early stages of regeneration. Proliferation of segments recommences and they are produced more rapidly than they are in the intact animal, as will be shown later.

Nitrogen-Excretion in Carcinides maenas (Pennant) during the Early Stages of Regeneration

Development ◽  
1955 ◽  
Vol 3 (3) ◽  
pp. 189-212
Author(s):  
A. E. Needham
Keyword(s):  
Wound Healing ◽  
Muscle Protein ◽  
Nitrogen Excretion ◽  
Regeneration Process ◽  
Critical Conditions ◽  
Skin Wounds ◽  
Subsequent Infection ◽  
Local Process ◽  
Early Stages ◽  
Two Alternatives

An increased excretion of nitrogen (hereafter referred to as N) by mammals in the early stages of wound-healing raises interesting problems (Cuthbertson, 1942, 1946; Needham, 1952). The first object of the present experiments was to ascertain if the phenomena associated with this N-“flow” (Cuthbertson, 1942, 1946) occur also in Carcinides, a, member of a very different group of animals. In mammals N-flow is greater than can be accounted for merely by the demolition of tissues damaged by the wound or by subsequent infection (Cuthbertson, 1942, 1946): the nitrogen/sulphur ratio in the urine indicates that after skin-wounds or burns much of the N comes from muscle-protein, and therefore that flow is a systematic, not a local process. It might be concerned with the provision of protein for the regeneration process itself, rather than with demolition. A Crustacean such as Carcinides provides critical conditions for testing these two alternatives.

Epidemiology of Potato Gangrene in Tasmania

1976 ◽  
Vol 24 (3) ◽  
pp. 337 ◽  
Author(s):  
RR Walker ◽  
GC Wade
Keyword(s):  
Wound Healing ◽  
High Temperatures ◽  
Fungal Isolate ◽  
Potato Tubers ◽  
Resistance Response ◽  
Phoma Exigua ◽  
Tuber Resistance ◽  
Tuber Maturity ◽  
Three Phases

In Tasmania, Phoma exigua Desm. var. exigua accounts for 90% of potential inoculum on tuber surfaces and 90% of gangrene lesions in stored potato tubers. P. exigua Desm, var, foveata (Foister) Boerema accounts for the remaining 10%. Tubers lifted from pre-senescent crops contain negligible levels of surface-borne inoculum and develop few lesions in storage. Washing removes only a small percentage of potential inoculum on tuber surfaces, which initiates infections if tubers are wounded and held in an environment unfavourable for wound healing. Tuber resistance to gangrene disease is determined by the interaction between tuber cultivar, tuber maturity and the nature of the fungal isolate within both varieties of P. exigua. The resistance response is greatest at high temperatures and is characterized by three phases: lesion retardation, lesion arrest and lesion rejection. Lesion rejection represents the end product of tuber resistance response and is associated with the development of post-infectional periderm.

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