In situ hybridization for the detection of human parvovirus B19 nucleic acid sequences in paraffin-embedded specimens

1990 ◽  
Vol 59 (1) ◽  
pp. 257-261 ◽  
Author(s):  
S. Hassam ◽  
J. Briner ◽  
J. D. Tratschin ◽  
G. Siegl ◽  
Ph. U. Heitz
Keyword(s):  
In Situ Hybridization ◽  
Nucleic Acid ◽  
Parvovirus B19 ◽  
Human Parvovirus B19 ◽  
Nucleic Acid Sequences

Intracellular localization of parvovirus B19 nucleic acid at the ultrastructural level by in situ hybridization with digoxigenin-labelled probes

1993 ◽  
Vol 25 (6) ◽  
pp. 421-429 ◽  
Author(s):  
A. L. Morey ◽  
D. J. P. Ferguson ◽  
K. O. Leslie ◽  
D. J. Taatjes ◽  
K. A. Fleming
Keyword(s):  
In Situ Hybridization ◽  
Nucleic Acid ◽  
Parvovirus B19 ◽  
Intracellular Localization ◽  
Ultrastructural Level

scholarly journals Peptide Nucleic Acid–Based In Situ Hybridization Assay for Detection of Parvovirus B19 Nucleic Acids

2006 ◽  
Vol 52 (6) ◽  
pp. 973-978 ◽  
Author(s):  
Francesca Bonvicini ◽  
Claudia Filippone ◽  
Elisabetta Manaresi ◽  
Giovanna Angela Gentilomi ◽  
Marialuisa Zerbini ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Nucleic Acid ◽  
Nucleic Acids ◽  
Peptide Nucleic Acid ◽  
Parvovirus B19 ◽  
Hybridization Assay ◽  
Pcr Assay ◽  
Clinical Specimens ◽  
Rna Molecules

Abstract Background: Peptide nucleic acid (PNA) molecules are known to bind complementary nucleic acid sequences with a much stronger affinity and with more stable binding than DNA or RNA molecules. We chose parvovirus B19, which is diagnosed by detection of nucleic acids by in situ hybridization assay (ISH) and/or PCR, as an experimental model to develop an ISH assay that uses biotinylated PNA probes to detect viral genome in clinical specimens. Methods: We first optimized the PNA-ISH assay on B19-infected and mock-infected UT-7/EpoS1 cells and then tested the assay on archival B19 specimens and on consecutive specimens. All data were compared with data obtained with a standardized DNA-based ISH assay and confirmed by a PCR-ELISA. Results: PNA-ISH detected B19 genome in a higher number of B19-infected UT-7/EpoS1 cells and with a more defined localization of viral nucleic acids than the standardized DNA-ISH assay. Moreover, PNA-ISH was able to detect B19 genome in all positive archival samples, whereas DNA-ISH failed in 5 samples. PNA-ISH detected more positive samples than DNA-ISH when consecutive specimens were analyzed, and a close agreement was found with PCR-ELISA results. Conclusions: The PNA-ISH assay had sensitivity and specificity comparable to a PCR assay and was more practical and quicker to perform than standard hybridization assays. The assay may be a suitable diagnostic test for the detection of viral nucleic acids in clinical specimens.

Histochemical aspects of nucleic acid detection

1995 ◽  
Vol 53 ◽  
pp. 746-747
Author(s):  
B. A. Hamkalo ◽  
Elizabeth R. Unger
Keyword(s):  
In Situ Hybridization ◽  
Nucleic Acid ◽  
High Resolution ◽  
Dna Sequences ◽  
Single Copy ◽  
Nucleic Acid Detection ◽  
Metaphase Chromosomes ◽  
Potential Applications ◽  
Nucleic Acid Sequences

This symposium brings together several approaches for the detection of specific nucleic acid sequences that have potential applications at the histochemical level.Trask et al. report on the use of fluorescence in situ hybridization (FISH) techniques to study the arrangement of DNA sequences in normal and diseaserelated chromosomes. The sites of specific DNA sequences can be fluorescently tagged. Different sequences can be labeled with different fluorochromes so that their arrangement can be studied using fluorescence microscopy. The distances between points on the same or different chromosomes can be determined in a large number of interphase nuclei or metaphase chromosomes. A variety of probe types, ranging from single-copy sequences to highly repeated sequences can be employed.Hamkalo and co-workers have used non-radioactive methods at the EM level for the detection of nucleic acid sequences by in situ hybridization. Analysis of metaphase chromosomes by electron microscopy allows for high resolution mapping of chromosomes. A variety of labelling procedures have been employed to illustrate the utility of high resolution nucleic acid sequence mapping in these preparations.

Subacute sclerosing panencephalitis: Measles virus matrix protein nucleic acid sequences detected by in situ hybridization

Neurology ◽  
1985 ◽  
Vol 35 (11) ◽  
pp. 1605-1605 ◽  
Author(s):  
P. Shapshak ◽  
W. W. Tourtellotte ◽  
S. Nakamura ◽  
M. C. Graves ◽  
M. Darvish ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Nucleic Acid ◽  
Measles Virus ◽  
Matrix Protein ◽  
Subacute Sclerosing Panencephalitis ◽  
Protein Nucleic Acid ◽  
Nucleic Acid Sequences

In situ hybridization to plant metaphase chromosomes using digoxigenin labeled nucleic acid sequences

1994 ◽  
pp. 349-361
Author(s):  
S. Hinnisdaels ◽  
I. Farbos ◽  
J. Del-Favero ◽  
J. Veuskens ◽  
M. Jacobs ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Nucleic Acid ◽  
Metaphase Chromosomes ◽  
Nucleic Acid Sequences
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