Effect of Mg, Zn and Mo salts on nitrate reductase activity and soluble protein content in leaves ofQuercus serrata

1994 ◽  
Vol 36 (4) ◽  
Author(s):  
M. K. Ghosh ◽  
R. C. Srivastava

2014 ◽  
Vol 71 (2) ◽  
pp. 101-107 ◽  
Author(s):  
Elżbieta Sacała ◽  
Anna Demczuk ◽  
Edward Grzyś ◽  
Anna Sobczak

The main objective of this study was to examine the influence of salinity on growth and biochemical parameters (NR activity, amino compound accumulation, protein content, some inorganic ion concentrations) of two varieties of maize (Limko and Koka). Salinity (75.0 mol • m<sup>-3</sup> NaCl and 37.5 mol • m<sup>-3</sup> Na<sub>2</sub>SO<sub>4</sub>) significantly reduced fresh and dry weights of the investigated maize varieties. NaCl induced higher reduction in biomass production than Na<sub>2</sub>SO<sub>4</sub>. Differences in salt tolerance between Limko and Koka were small.It was found that in both maize varieties the nitrate reductase activity decreased under salt stress (100 mol • m<sup>-3</sup> NaCl). This effect was more marked in the Koka variety than Limko. Decrease in nitrate reductase activity had no inhibitory effect on accumulation of protein and free amino compounds. Salinity treatment (100 mol • m<sup>-3</sup> NaCl) led to an increase in free amino compound contents in roots and shoots of both investigated maize varieties, but these changes were not very large. The highest increase in amino compound level was observed in roots of Koka and it was 2-fold higher than in control plants. Salt stress did not change soluble protein contents in Limko and Koka varieties with the exception of Limko leaves. There was significant increase in soluble protein content on leaves of Limko and it amounted 128% of the control. Salinity caused a great increase in leaf and root Na<sup>+</sup> concentration and a decrease in case of Ca<sup>2+</sup> and K<sup>+</sup> contents. The declines in Ca<sup>2+</sup> content in maize roots were 76 and 70% respectively for Koka and Limko roots. The fall in K<sup>+</sup> concentration was high, but not so drastic as in Ca<sup>2+</sup>. In roots of Koka and Limko the reduction in K<sup>+</sup> content was approximately 30%. Higher reduction in K<sup>+</sup>content showed shoots of Limko. In this case K<sup>+</sup> content lowered to 49% of control.



Author(s):  
V. Divyabharathi ◽  
V. Swaminathan ◽  
P. Paramaguru ◽  
K. Venkatesan ◽  
T. Anitha ◽  
...  

A trial was conducted to assess the physiological and biochemical changes in M. oleifera (L.) Millsp.cv. PKM 1 induced by heading back and pinching treatments. Chlorophyll content, nitrate reductase content, soluble protein content and total phenol content were assessed after the new shoot initiated from heading back and pruning treatments before the flower initiation. Height and stage at which the apical growth was arrested significantly influenced chlorophyll, nitrate reductase, protein and phenol content in leaves. Heading back at 70 cm combined with pinching 100 days after heading back greatly influenced the physiological and biochemical factors except for soluble protein as it was increased by heading back at 30 cm.



2015 ◽  
Vol 46 (2) ◽  
pp. 275-283
Author(s):  
A. Suder-Moraw ◽  
J. Buczek

A 3:30 Ca:Mg ratio in the nutrient solution produces in tomato seedlings symptoms of Ca<sup>2+</sup> deficit owing to excessive accumulation of Mg<sup>2+</sup> ions and the depressed Ca<sup>2+</sup> accumulation. As a result of this a decrease in dry weight increment and protein content is observed together with inhibition of nitrate reductase activity. A doubled Ca<sup>2+</sup> dose in the nutrient solution, that is a change in the Ca:Mg ratio to 6:30 abolishes the external symptoms of Ca<sup>2+</sup> deficit and reduces Mg<sup>2+</sup> accumulation, that of Ca<sup>2+</sup> ions remaining unchanged. At the same time an enhanced activity of nitrate reductase appears, reaching values close to those in control plants. Tomato seedlings grown on a 3-fold increased Ca<sup>2+</sup> dose (Ca:Mg = 9:30) did not differ at all from the control ones. An in-crease in calcium concentration in the nutrient solution, the high magnesium dose remaining unchanged, causes enhanced K<sup>+</sup> accumulation, and this may affect nitrate absorption and reduction. It would seem that Ca<sup>2+</sup> deficit in plant tissues induced by excessive Mg<sup>2+</sup> accumulation with unsuitable Ca:Mg ratio in the nutrient solution in cause of disorders in NO<sub>3</sub> nitrogen assimilation.



2017 ◽  
Vol 14 (3) ◽  
pp. 671-681 ◽  
Author(s):  
Zhiguang Xu ◽  
Guang Gao ◽  
Juntian Xu ◽  
Hongyan Wu

Abstract. The development of golden tides is potentially influenced by global change factors, such as ocean acidification and eutrophication, but related studies are very scarce. In this study, we cultured a golden tide alga, Sargasssum muticum, at two levels of pCO2 (400 and 1000 µatm) and phosphate (0.5 and 40 µM) to investigate the interactive effects of elevated pCO2 and phosphate on the physiological properties of the thalli. Higher pCO2 and phosphate (P) levels alone increased the relative growth rate by 41 and 48 %, the net photosynthetic rate by 46 and 55 %, and the soluble carbohydrates by 33 and 62 %, respectively, while the combination of these two levels did not promote growth or soluble carbohydrates further. The higher levels of pCO2 and P alone also enhanced the nitrate uptake rate by 68 and 36 %, the nitrate reductase activity (NRA) by 89 and 39 %, and the soluble protein by 19 and 15 %, respectively. The nitrate uptake rate and soluble protein was further enhanced, although the nitrate reductase activity was reduced when the higher levels of pCO2 and P worked together. The higher pCO2 and higher P levels alone did not affect the dark respiration rate of the thalli, but together they increased it by 32 % compared to the condition of lower pCO2 and lower P. The neutral effect of the higher levels of pCO2 and higher P on growth and soluble carbohydrates, combined with the promoting effect on soluble protein and dark respiration, suggests that more energy was drawn from carbon assimilation to nitrogen assimilation under conditions of higher pCO2 and higher P; this is most likely to act against the higher pCO2 that caused acid–base perturbation via synthesizing H+ transport-related protein. Our results indicate that ocean acidification and eutrophication may not boost golden tide events synergistically, although each one has a promoting effect.



Sign in / Sign up

Export Citation Format

Share Document