Ascorbic acid biosensor using ascorbate oxidase immobilized on alkylamine glass beads

1992 ◽  
Vol 32 (1-3) ◽  
pp. 73-78 ◽  
Author(s):  
E. T. A. Marques ◽  
J. L. Lima Filho
Keyword(s):  
Ascorbic Acid ◽  
Glass Beads ◽  
Ascorbate Oxidase ◽  
Alkylamine Glass

Ascorbic acid determination in biological fluids using ascorbate oxidase immobilized on alkylamine glass beads in a flow injection potentiometric system

1994 ◽  
Vol 44 (1) ◽  
pp. 81-89 ◽  
Author(s):  
I. D. H. C. Marques ◽  
E. T. A. Marques ◽  
A. C. Silva ◽  
W. M. Ledingham ◽  
E. H. M. Melo ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Flow Injection ◽  
Biological Fluids ◽  
Glass Beads ◽  
Ascorbate Oxidase ◽  
Ascorbic Acid Determination ◽  
Alkylamine Glass ◽  
Acid Determination

Amperometric assay of vitamin C using an ascorbate oxidase enzyme electrode

1979 ◽  
Vol 44 (11) ◽  
pp. 3395-3404 ◽  
Author(s):  
Pavel Posádka ◽  
Lumír Macholán
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Oxygen Electrode ◽  
Ascorbate Oxidase ◽  
Current Response ◽  
Reaction Conditions ◽  
Long Term Storage ◽  
Oxidase Enzyme ◽  
Optimal Reaction

An oxygen electrode of the Clark type, coated by a thin, active layer of chemically insolubilized ascorbate oxidase from squash peelings specifically detects by measuring oxygen uptake 10 to 400 μg of ascorbic acid in 3 ml of phosphate buffer. The record of current response to substrate addition lasts 1-2 min. The ascorbic acid values determined in various samples of fruit juices are in good agreement with the data obtained by titration and polarography. The suitable composition of the membrane and its lifetime and stability during long-term storage are described; optimal reaction conditions of vitamin C determination and the possibilities of interference of other compounds are also examined. Of the 35 phenols, aromatic amines and acids tested chlorogenic acid only can cause a positive error provided that the enzyme membrane has been prepared from ascorbate oxidase of high purity.

A Novel Design and Fabrication of Ascorbic Acid Sensitive Biosensor Based on Combination of HAP/rGO/AuNPs Composite and Ascorbate Oxidase

2019 ◽  
Vol 31 (2) ◽  
pp. 337-346 ◽  
Author(s):  
Chenyu Zhao ◽  
Junfeng Jiao ◽  
Wencui Zhou ◽  
Yong Zhang ◽  
Han Liu ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Ascorbate Oxidase ◽  
Novel Design

Fabrication of Ascorbic Acid Biosensor Based on Coupling Polyethylene Glycol Modified Silk Fibroin Membrane onto Glassy Carbon Electrode

2016 ◽  
Vol 835 ◽  
pp. 63-70
Author(s):  
Pacharawan Ratanasongtham ◽  
Lalida Shank ◽  
Jaroon Jakmunee ◽  
Ruangsri Watanesk ◽  
Surasak Watanesk
Keyword(s):  
Ascorbic Acid ◽  
Polyethylene Glycol ◽  
Glassy Carbon Electrode ◽  
Silk Fibroin ◽  
Glassy Carbon ◽  
Phosphate Buffer ◽  
Storage Time ◽  
Initial Response ◽  
Ascorbate Oxidase ◽  
Carbon Electrode

Nowadays biosensors have been extensively used in a wide variety of applications especially in clinical works and food industry. In this work, a specific ascorbic acid (AA) biosensor was developed by immobilizing ascorbate oxidase (ASOD) on a polyethylene glycol (PEG) modified silk fibroin (SF) membrane then coupling to the glassy carbon electrode (GCE). The SF-PEG-ASOD membrane provided the highest enzyme activity in phosphate buffer at pH 5. As being the electrode, the SF-PEG-ASOD modified GCE displayed the highest response when it is operated under the condition of 0.40 mg/L of ASOD in phosphate buffer at pH 5. This biosensor provided both good linearity (r2 = 0.999 in the range of 1.0-10.0 mM) and sensitivity with short response time (26s). It also exhibited good anti-interference ability with the storage time of 5 days without changing its initial response.

scholarly journals Ascorbate Oxidase Minimizes Interference by High-Concentration Ascorbic Acid in Total Cholesterol Assays

2016 ◽  
Vol 36 (2) ◽  
pp. 188-190 ◽  
Author(s):  
Hyunjin Nah ◽  
Jisook Yim ◽  
Sang-Guk Lee ◽  
Jong-Baeck Lim ◽  
Jeong-Ho Kim
Keyword(s):  
Ascorbic Acid ◽  
Total Cholesterol ◽  
Ascorbate Oxidase ◽  
High Concentration
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