Conditions for the extraction of DNA and RNA from tobacco pollen by sodium chloride and perchloric acid

1972 ◽  
Vol 14 (6) ◽  
pp. 385-389 ◽  
Author(s):  
J. Süss
Keyword(s):  
Sodium Chloride ◽  
Perchloric Acid ◽  
Dna And Rna ◽  
Tobacco Pollen

THE ASSOCIATION OF [6,7-3H]OESTRADIOL WITH A NUCLEAR PROTEIN

1967 ◽  
Vol 39 (4) ◽  
pp. 533-542 ◽  
Author(s):  
R. J. B. KING ◽  
J. GORDON
Keyword(s):  
Sodium Chloride ◽  
Anterior Pituitary ◽  
Nuclear Protein ◽  
Sodium Deoxycholate ◽  
Uterine Epithelium ◽  
Optimal Concentration ◽  
Mammary Tumour ◽  
Receptor Complex ◽  
Dna And Rna

SUMMARY The binding of [6,7-3H]oestradiol to nuclei from rat dimethylbenzanthracene-induced (DMBA) mammary tumour, uterine epithelium, anterior pituitary and kidney has been studied. Digestion of nuclei labelled in vivo with [6,7-3H]oestradiol, with RNase, DNase and trypsin indicated that only the last enzyme released tritium from the nucleus, indicating that the receptor is a protein. The optimal concentration of sodium chloride for extracting the oestradiol-receptor complex from tumour nuclei was 1-molar. This concentration was required even after enzymic removal of most of the DNA and RNA. Sodium deoxycholate also extracted oestradiol from nuclei but at the same time dissociated the oestradiol-receptor complex. An attempt was made to fractionate the oestradiol-receptor complex on Sephadex and Biogel columns.

The Solubility of Lead Chloride in Sodium Chloride, Perchloric Acid and Hydrochloric Acid Solutions1

1953 ◽  
Vol 75 (11) ◽  
pp. 2768-2768 ◽  
Author(s):  
S. Z. Lewin ◽  
John E. Vance ◽  
Lawrence B. Nelson
Keyword(s):  
Sodium Chloride ◽  
Hydrochloric Acid ◽  
Perchloric Acid ◽  
Lead Chloride

Extended Preservation of Iron for Transmission

1967 ◽  
Vol 25 ◽  
pp. 354-355
Author(s):  
E. P. Abrahamson II ◽  
M. W. Dumais
Keyword(s):  
Acid Solution ◽  
Perchloric Acid ◽  
Microscopy Study ◽  
Perchloric Acid Solution ◽  
Transmission Microscopy ◽  
Iron Base ◽  
Cold Stage

In a transmission microscopy study of iron and dilute iron base alloys, it was determined that it is possible to preserve specimens for extended periods of time. Our specimens were prepunched from 5 to 8 mil sheet to microscope size and annealed for several hours at 700°C. They were then thinned in a glacial acetic-12 percent perchloric acid solution using 10 volts and 20 milliamperes, at a temperature of 8 to 14°C.It was noted that by the use of a cold stage, the same specimen can be observed for periods up to one week without excess contamination. When removal of the specimen from the column becomes necessary, it was observed that a specimen may be kept for later observation in 1,2 dichloroethene or methanol for periods in excess of two weeks.

High-resolution nucleic acid sequence mapping via in situ hybridization at the Electron Microscope level

1995 ◽  
Vol 53 ◽  
pp. 752-753
Author(s):  
B.A. Hamkalo ◽  
S. Narayanswami ◽  
A.P. Kausch
Keyword(s):  
Nucleic Acid ◽  
Interphase Nucleus ◽  
Genome Mapping ◽  
Precise Location ◽  
Electron Microscope Level ◽  
Rna Sequences ◽  
Fundamental Interest ◽  
Whole Cells ◽  
Dna And Rna

The availability of nonradioactive methods to label nucleic acids an the resultant rapid and greater sensitivity of detection has catapulted the technique of in situ hybridization to become the method of choice to locate of specific DNA and RNA sequences on chromosomes and in whole cells in cytological preparations in many areas of biology. It is being applied to problems of fundamental interest to basic cell and molecular biologists such as the organization of the interphase nucleus in the context of putative functional domains; it is making major contributions to genome mapping efforts; and it is being applied to the analysis of clinical specimens. Although fluorescence detection of nucleic acid hybrids is routinely used, certain questions require greater resolution. For example, very closely linked sequences may not be separable using fluorescence; the precise location of sequences with respect to chromosome structures may be below the resolution of light microscopy(LM); and the relative positions of sequences on very small chromosomes may not be feasible.

Scanning tunneling microscopy (STM) of DNA and RNA

1989 ◽  
Vol 47 ◽  
pp. 34-35
Author(s):  
Patricia G. Arscott ◽  
Gil Lee ◽  
Victor A. Bloomfield ◽  
D. Fennell Evans
Keyword(s):  
Molecular Structures ◽  
Inorganic Materials ◽  
Resolving Power ◽  
Scanning Tunneling ◽  
Tunneling Microscopy ◽  
Form And Function ◽  
Dna And Rna ◽  
Calf Thymus ◽  
And Function ◽  
The Relationship

STM is one of the most promising techniques available for visualizing the fine details of biomolecular structure. It has been used to map the surface topography of inorganic materials in atomic dimensions, and thus has the resolving power not only to determine the conformation of small molecules but to distinguish site-specific features within a molecule. That level of detail is of critical importance in understanding the relationship between form and function in biological systems. The size, shape, and accessibility of molecular structures can be determined much more accurately by STM than by electron microscopy since no staining, shadowing or labeling with heavy metals is required, and there is no exposure to damaging radiation by electrons. Crystallography and most other physical techniques do not give information about individual molecules.We have obtained striking images of DNA and RNA, using calf thymus DNA and two synthetic polynucleotides, poly(dG-me5dC)·poly(dG-me5dC) and poly(rA)·poly(rU).

Human detectability thresholds for saccharin, sodium saccharin, and sodium chloride.

1970 ◽  
Vol 70 (1, Pt.1) ◽  
pp. 113-115 ◽  
Author(s):  
Ruth A. Williams
Keyword(s):  
Sodium Chloride ◽  
Saccharin Sodium ◽  
Sodium Saccharin

The increase in intracellular free-lysine levels of growing Baker's yeast (Saccharomyces cerevisiae) by sodium chloride

1981 ◽  
Vol 31 (1) ◽  
pp. 290-294 ◽  
Author(s):  
Robert D. Tanner ◽  
L. Daniel Richmond ◽  
Chia-Jenn Wei ◽  
Jonathan Woodward
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Sodium Chloride ◽  
Baker’S Yeast ◽  
Baker's Yeast ◽  
Yeast Saccharomyces Cerevisiae
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