Mapping of adult plant stripe rust resistance genes in diploid A genome wheat species and their transfer to bread wheat

Wheat stripe (yellow) rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a serious fungal disease worldwide, especially in the Huang-Huai-Hai region, a main wheat production area in China. Gene postulation, molecular testing, and pedigree analysis were conducted to determine the presence of stripe rust resistance genes to 15 Pst races in 66 selected commercial wheat cultivars released from 2000 to 2016. In addition, races CYR32, CYR33, and CYR34 were used to evaluate resistance to Pst at the adult-plant stage of wheat in the field. Four Yr genes (Yr9, Yr10, Yr26, and Yr32) were postulated in 24 wheat cultivars either singly or in combination. Thirty-six cultivars might contain unknown Yr genes, whereas no identified Yr gene was postulated in six cultivars. Yr9 was detected at a frequency of 28.8%, and no cultivars carried Yr5, Yr15, or Yr18. Ten cultivars (15.2%) exhibited adult-plant resistance in the field tests with three predominant races. Three cultivars (Langyan 43, Xinong 889, and Yunfeng 139) had all-stage resistance. These results are useful to growers selecting cultivars and to breeders aiming to use more resistance genes to develop new cultivars with effective resistance in order to reduce stripe rust damage.

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Marker Assisted Transfer of Stripe Rust and Stem Rust Resistance Genes into Four Wheat Cultivars

Agronomy ◽

10.3390/agronomy9090497 ◽

2019 ◽

Vol 9 (9) ◽

pp. 497 ◽

Cited By ~ 8

Author(s):

Mandeep S. Randhawa ◽

Navtej S. Bains ◽

Virinder S. Sohu ◽

Parveen Chhuneja ◽

Richard M. Trethowan ◽

...

Keyword(s):

Stripe Rust ◽

Resistance Genes ◽

Stem Rust ◽

Rust Resistance ◽

Stem Rust Resistance ◽

Stripe Rust Resistance ◽

Adult Plant ◽

Wheat Cultivars ◽

Stem Rust Resistance Genes ◽

Rust Resistance Genes

Three rust diseases namely; stem rust caused by Puccinia graminis f. sp. tritici (Pgt), leaf rust caused by Puccinia triticina (Pt), and stripe rust caused by Puccinia striiformis f. sp. tritici (Pst), are the most common fungal diseases of wheat (Triticum aestivum L.) and cause significant yield losses worldwide including Australia. Recently characterized stripe rust resistance genes Yr51 and Yr57 are effective against pre- and post-2002 Pst pathotypes in Australia. Similarly, stem rust resistance genes Sr22, Sr26, and Sr50 are effective against the Pgt pathotype TTKSK (Ug99) and its derivatives in addition to commercially important Australian pathotypes. Effectiveness of these genes make them good candidates for combining with known pleiotropic adult plant resistance (PAPR) genes to achieve durable resistance against three rust pathogens. This study was planned to transfer rust resistance genes Yr51, Yr57, Sr22, Sr26, and Sr50 into two Australian (Gladius and Livingston) and two Indian (PBW550 and DBW17) wheat cultivars through marker assisted selection (MAS). These cultivars also carry other rust resistance genes: Gladius carries Lr37/Yr17/Sr38 and Sr24/Lr24; Livingston carries Lr34/Yr18/Sr57, Lr37/Yr17/Sr38, and Sr2; PBW550 and DBW17 carry Lr34/Yr18/Sr57 and Lr26/Yr9/Sr31. Donor sources of Yr51 (AUS91456), Yr57 (AUS91463), Sr22 (Sr22/3*K441), Sr26 (Sr26 WA1), and Sr50 (Dra-1/Chinese Spring ph1b/2/3* Gabo) were crossed with each of the recurrent parents to produce backcross progenies. Markers linked to Yr51 (sun104), Yr57 (gwm389 and BS00062676), Sr22 (cssu22), Sr26 (Sr26#43), and Sr50 (Sr50-5p-F3, R2) were used for their MAS and markers csLV34 (Lr34/Yr18/Sr57), VENTRIUP-LN2 (Lr37/Yr17/Sr38), Sr24#12 (Sr24/Lr24), and csSr2 (Sr2) were used to select genes present in recurrent parents. Progenies of selected individuals were grown and selected under field conditions for plant type and adult plant rust responses. Final selections were genotyped with the relevant markers. Backcross derivatives of these genes were distributed to breeding companies for use as resistance donors.

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Markers assisted selection for multiple Stripe rust resistance genes in spring bread wheat lines

International Journal of Biosciences (IJB) ◽

10.12692/ijb/8.3.63-74 ◽

2016 ◽

Vol 8 (3) ◽

pp. 63-74 ◽

Cited By ~ 1

Keyword(s):

Bread Wheat ◽

Stripe Rust ◽

Resistance Genes ◽

Rust Resistance ◽

Stripe Rust Resistance ◽

Spring Bread Wheat ◽

Selection For ◽

Wheat Lines ◽

Rust Resistance Genes

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Assessment of Genetic Diversity for Stem Rust and Stripe Rust Resistance in an International Wheat Nursery Using Phenotypic and Molecular Technologies

Uganda Journal of Agricultural Sciences ◽

10.4314/ujas.v20i1.1 ◽

2021 ◽

Vol 20 (1) ◽

pp. 1-27

Author(s):

Bosco Chemayek ◽

Urmil K. Bansal ◽

Hanif Miah ◽

William W. Wagoire ◽

Harbans S. Bariana

Keyword(s):

Molecular Markers ◽

Stripe Rust ◽

Resistance Genes ◽

Stem Rust ◽

Rust Resistance ◽

Stem Rust Resistance ◽

Stripe Rust Resistance ◽

Adult Plant ◽

Stem Rust Resistance Genes ◽

Rust Resistance Genes

The objective of this study was to assess diversity for stem rust and stripe rust resistance in an international wheat screening nursery under greenhouse conditions using pathotypes with known avirulence/ virulence profiles. A set of 95 entries of an international wheat screening nursery collected from material generated by staff of the International Maize and Wheat Improvement Centre (CIMMYT) was tested against seven Australian Pgt and five Pst pathotypes through artificial inoculation under the greenhouse conditions using standard procedures. Ten all-stage stem rust resistance genes (Sr8a, Sr8b, Sr9b, Sr12, Sr17, Sr23, Sr24, Sr30, Sr31 and Sr38) and seven all-stage stripe rust resistance genes (Yr3, Yr4, Yr6, Yr9, Yr17, Yr27 and Yr34) were postulated either singly or in combinations based on seedling responses of test entries against pathotypes differing in virulence for commonly deployed genes. Sr30 and Sr38 were the most common stem rust resistance genes in this nursery. The Sr38-linked stripe rust resistance gene Yr17 was present in high proportion. The presence of rust resistance genes Sr24, Sr31/Yr9, Sr38/Yr17 and Yr4 were confirmed using the closely linked molecular markers. The adult plant resistance (APR) genes Sr2 and Lr34/Yr18/Sr57 were detected using linked molecular markers csSr2 and csLV34, respectively. Genotypes carrying combinations of stem rust and stripe rust resistance were identified for use as donor sources in breeding programs.

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Characterization of Wheat Stripe Rust Resistance Genes in Shaanmai 139

ACTA AGRONOMICA SINICA ◽

10.3724/sp.j.1006.2010.00109 ◽

2010 ◽

Vol 36 (1) ◽

pp. 109-114 ◽

Cited By ~ 8

Author(s):

Hong ZHANG ◽

Zhi-Long REN ◽

Yin-Gang HU ◽

Chang-You WANG ◽

Wan-Quan JI

Keyword(s):

Stripe Rust ◽

Resistance Genes ◽

Rust Resistance ◽

Stripe Rust Resistance ◽

Wheat Stripe Rust ◽

Rust Resistance Genes

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Allelic analysis of stripe rust resistance genes on wheat chromosome 2BS

Genome ◽

10.1139/g08-079 ◽

2008 ◽

Vol 51 (11) ◽

pp. 922-927 ◽

Cited By ~ 35

Author(s):

P. G. Luo ◽

X. Y. Hu ◽

Z. L. Ren ◽

H. Y. Zhang ◽

K. Shu ◽

...

Keyword(s):

Ssr Markers ◽

Stripe Rust ◽

Resistance Genes ◽

Rust Resistance ◽

Dominant Gene ◽

Wheat Chromosome ◽

Stripe Rust Resistance ◽

Resistance Response ◽

Rust Resistance Genes ◽

Yr Genes

Stripe rust, caused by Puccinia striiormis Westend f. sp. tritici, is one of the most important foliar diseases of wheat ( Triticum aestivum L.) worldwide. Stripe rust resistance genes Yr27, Yr31, YrSp, YrV23, and YrCN19 on chromosome 2BS confer resistance to some or all Chinese P. striiormis f. sp. tritici races CYR31, CYR32, SY11-4, and SY11-14 in the greenhouse. To screen microsatellite (SSR) markers linked with YrCN19, F1, F2, and F3 populations derived from cross Ch377/CN19 were screened with race CYR32 and 35 SSR primer pairs. Linkage analysis indicated that the single dominant gene YrCN19 in cultivar CN19 was linked with SSR markers Xgwm410, Xgwm374, Xwmc477, and Xgwm382 on chromosome 2BS with genetic distances of 0.3, 7.9, 12.3, and 21.2 cM, respectively. Crosses of CN19 with wheat lines carrying other genes on chromosome 2B showed that all were located at different loci. YrCN19 is thus different from the other reported Yr genes in chromosomal location and resistance response and was therefore named Yr41. Prospects and strategies of using Yr41 and other Yr genes in wheat improvement for stripe rust resistance are discussed.

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Genome-wide association analysis of stripe rust resistance in modern Chinese wheat

BMC Plant Biology ◽

10.1186/s12870-020-02693-w ◽

2020 ◽

Vol 20 (1) ◽

Author(s):

Mengjie Jia ◽

Lijun Yang ◽

Wei Zhang ◽

Garry Rosewarne ◽

Junhui Li ◽

...

Keyword(s):

Stripe Rust ◽

Rust Resistance ◽

Genome Wide Association ◽

Stripe Rust Resistance ◽

Adult Plant ◽

Amplification Refractory Mutation System ◽

Post Inoculation ◽

Genome Wide ◽

A Genome ◽

Chinese Wheat

Abstract Background Stripe rust (yellow rust) is a significant disease for bread wheat (Triticum aestivum L.) worldwide. A genome-wide association study was conducted on 240 Chinese wheat cultivars and elite lines genotyped with the wheat 90 K single nucleotide polymorphism (SNP) arrays to decipher the genetic architecture of stripe rust resistance in Chinese germplasm. Results Stripe rust resistance was evaluated at the adult plant stage in Pixian and Xindu in Sichuan province in the 2015–2016 cropping season, and in Wuhan in Hubei province in the 2013–2014, 2016–2017 and 2018–2019 cropping seasons. Twelve stable loci for stripe rust resistance were identified by GWAS using TASSEL and GAPIT software. These loci were distributed on chromosomes 1B, 1D, 2A, 2B, 3A, 3B, 4B (3), 4D, 6D, and 7B and explained 3.6 to 10.3% of the phenotypic variation. Six of the loci corresponded with previously reported genes/QTLs, including Sr2/Yr30/Lr27, while the other six (QYr.hbaas-1BS, QYr.hbaas-2BL, QYr.hbaas-3AL, QYr.hbaas-4BL.3, QYr.hbaas-4DL, and QYr.hbaas-6DS) are probably novel. The results suggest high genetic diversity for stripe rust resistance in this population. The resistance alleles of QYr.hbaas-2AS, QYr.hbaas-3BS, QYr.hbaas-4DL, and QYr.hbaas-7BL were rare in the present panel, indicating their potential use in breeding for stripe rust resistance in China. Eleven penta-primer amplification refractory mutation system (PARMS) markers were developed from SNPs significantly associated with seven mapped QTLs. Twenty-seven genes were predicted for mapped QTLs. Six of them were considered as candidates for their high relative expression levels post-inoculation. Conclusion The resistant germplasm, mapped QTLs, and PARMS markers developed in this study are resources for enhancing stripe rust resistance in wheat breeding.

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Molecular Marker Based Design for Breeding Wheat Lines with Multiple Resistance and Superior Quality

Plant Disease ◽

10.1094/pdis-02-20-0420-re ◽

2020 ◽

Vol 104 (10) ◽

pp. 2658-2664

Author(s):

Tao Liu ◽

George Fedak ◽

Lianquan Zhang ◽

Rangrang Zhou ◽

Dawn Chi ◽

...

Keyword(s):

Stripe Rust ◽

Resistance Genes ◽

High Resistance ◽

Stem Rust ◽

Rust Resistance ◽

Stripe Rust Resistance ◽

Multiple Resistance ◽

Wheat Stem Rust ◽

Wheat Production ◽

Rust Resistance Genes

There has not been a major wheat stem rust epidemic worldwide since the 1970s, but the emergence of race TTKSK of Puccinia graminis f. sp. tritici in 1998 presented a great threat to the world wheat production. Single disease-resistance genes are usually effective for only several years before the pathogen changes genetically to overcome the resistance. Stripe rust caused by Puccinia striiformis f. sp. tritici (Pst) is one of the most common and persistent wheat diseases worldwide. The development of varieties with multiple resistance is the most economical and effective strategy for preventing stripe rust and stem rust, the two main rust diseases constraining wheat production. Plateau 448 has been widely used in the spring wheat growing region in northwest China, but it has become susceptible to stripe rust and is susceptible to TTKSK. To produce more durable resistance to race TTKSK as well as to stripe rust, four stem rust resistance genes (Sr33, Sr36, Sr-Cad, and Sr43) and three stripe rust resistance genes (Yr5, Yr18, and Yr26) were simultaneously introgressed into Plateau 448 to improve its stem rust (Ug99) and stripe rust resistance using a marker-assisted backcrossing strategy combined with phenotypic selection. We obtained 131 BC1F5 lines that pyramided two to four Ug99 resistance genes and one to two Pst resistance genes simultaneously. Thirteen of these lines were selected for their TTKSK resistance, and all of them exhibited near immunity or high resistance to TTKSK. Among the 131 pyramided lines, 95 showed high resistance to mixed Pst races. Nine lines exhibited not only high resistance to TTKSK and Pst but also better agronomic traits and high-molecular-weight glutenin subunit compositions than Plateau 448.

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