Multiple alleles for resistance and susceptibility modulate the defense response in the interaction of tetraploid potato (Solanum tuberosum) with Synchytrium endobioticum pathotypes 1, 2, 6 and 18

Abstract A description is provided for Synchytrium endobioticum. Information is included on the disease caused by the organism, its transmission, geographical distribution, and hosts. HOSTS: Solanaceae (potato, Solanum tuberosum). Other plants infected experimentally include several species of Solanum (Karling, 1964), Petunia and Nicotiana spp. (53, 4566), Lycopersicon esculentum, Physalis and Capsicastrum spp. (59, 416). DISEASE: Potato wart disease, causing dark brown warty cauliflower-like excrescences of infected tubers, which decay to release golden-brown resting spores. On aerial shoots green galls may develop, composed of convoluted masses of tissue. GEOGRAPHICAL DISTRIBUTION: Africa, Asia, Europe, North America, South America (CMI Map 1, ed. 5, 1972). TRANSMISSION: By the movement of infected soil or plant tissues. Local dispersal by zoospores swimming in water films may also occur.

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Exogenous SA Initiated Defense Response and Multi-signaling Pathway in Tetraploid Potato SD20

Horticultural Plant Journal ◽

10.1016/j.hpj.2020.01.003 ◽

2020 ◽

Vol 6 (2) ◽

pp. 99-110

Author(s):

Jiayi Zheng ◽

Yu Yang ◽

Xiao Guo ◽

Liping Jin ◽

Xingyao Xiong ◽

...

Keyword(s):

Signaling Pathway ◽

Defense Response ◽

Tetraploid Potato

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The Solanum tuberosum GBSSI gene: a target for assessing gene and base editing in tetraploid potato

10.1101/628107 ◽

2019 ◽

Author(s):

Florian Veillet ◽

Laura Chauvin ◽

Marie-Paule Kermarrec ◽

François Sevestre ◽

Mathilde Merrer ◽

...

Keyword(s):

Solanum Tuberosum ◽

Genome Editing ◽

Genome Engineering ◽

Basic Research ◽

Loss Of Function ◽

Dna Breaks ◽

Nucleotide Substitutions ◽

Discrete Variation ◽

Tetraploid Potato ◽

Base Editing

AbstractGenome editing has recently become a method of choice for basic research and functional genomics, and holds great potential for molecular plant breeding applications. The powerful CRISPR-Cas9 system that typically produces double-strand DNA breaks is mainly used to generate knockout mutants. Recently, the development of base editors has broadened the scope of genome editing, allowing precise and efficient nucleotide substitutions. In this study, we produced mutants in two cultivated elite cultivars of the tetraploid potato (Solanum tuberosum) using stable or transient expression of the CRISPR-Cas9 components to knockout the amylose-producing StGBSSI gene. We set up a rapid, highly sensitive and cost-effective screening strategy based on high-resolution melting analysis followed by direct Sanger sequencing and trace chromatogram analysis. Most mutations consisted of small indels, but unwanted insertions of plasmid DNA were also observed. We successfully created tetra-allelic mutants with impaired amylose biosynthesis, confirming the loss-of-function of the StGBSSI protein. The second main objective of this work was to demonstrate the proof of concept of CRISPR-Cas9 base editing in the tetraploid potato by targeting two loci encoding catalytic motifs of the StGBSSI enzyme. Using a cytidine base editor (CBE), we efficiently and precisely induced DNA substitutions in the KTGGL-encoding locus, leading to discrete variation in the amino acid sequence and generating a loss-of-function allele. The successful application of base editing in the tetraploid potato opens up new avenues for genome engineering in this species.Key MessageThe StGBSSI gene was successfully and precisely edited in the tetraploid potato using gene and base editing strategies, leading to plants with impaired amylose biosynthesis.

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