Genome-wide association mapping of leaf rust and stripe rust resistance in wheat accessions using the 90K SNP array

2021 ◽  
Author(s):  
Peipei Zhang ◽  
Xiaocui Yan ◽  
Takele-Weldu Gebrewahid ◽  
Yue Zhou ◽  
Ennian Yang ◽  
...  
Keyword(s):  
Association Mapping ◽  
Leaf Rust ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Snp Array ◽  
Genome Wide Association ◽  
Stripe Rust Resistance ◽  
Genome Wide ◽  
90K Snp Array

scholarly journals Genome-wide association mapping reveals a rich genetic architecture of stripe rust resistance loci in emmer wheat (Triticum turgidum ssp. dicoccum)

2017 ◽  
Vol 130 (11) ◽  
pp. 2249-2270 ◽  
Author(s):  
Weizhen Liu ◽  
Marco Maccaferri ◽  
Xianming Chen ◽  
Gaetano Laghetti ◽  
Domenico Pignone ◽  
...  
Keyword(s):  
Association Mapping ◽  
Stripe Rust ◽  
Genetic Architecture ◽  
Rust Resistance ◽  
Triticum Turgidum ◽  
Emmer Wheat ◽  
Genome Wide Association ◽  
Stripe Rust Resistance ◽  
Genome Wide

scholarly journals Genome wide association mapping through 90K SNP array against Leaf rust pathogen in bread wheat genotypes under field conditions

2021 ◽  
pp. 101628
Author(s):  
Hafiz Ghulam Muhu-Din Ahmed ◽  
Muhammad Nouman Iqbal ◽  
Muhammad Arslan Iqbal ◽  
Yawen Zeng ◽  
Aziz Ullah ◽  
...  
Keyword(s):  
Association Mapping ◽  
Leaf Rust ◽  
Bread Wheat ◽  
Snp Array ◽  
Field Conditions ◽  
Genome Wide Association ◽  
Wheat Genotypes ◽  
Genome Wide ◽  
Rust Pathogen ◽  
90K Snp Array

scholarly journals Genome wide association mapping of stripe rust resistance in Afghan wheat landraces

Plant Science ◽  
2016 ◽  
Vol 252 ◽  
pp. 222-229 ◽  
Author(s):  
Alagu Manickavelu ◽  
Reem Joukhadar ◽  
Abdulqader Jighly ◽  
Caixia Lan ◽  
Julio Huerta-Espino ◽  
...  
Keyword(s):  
Association Mapping ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Genome Wide Association ◽  
Stripe Rust Resistance ◽  
Genome Wide ◽  
Wheat Landraces

scholarly journals Identifying Loci Conferring Resistance to Leaf and Stripe Rusts in a Spring Wheat Population (Triticum aestivum) via Genome-Wide Association Mapping

2019 ◽  
Vol 109 (11) ◽  
pp. 1932-1940 ◽  
Author(s):  
Weizhen Liu ◽  
James Kolmer ◽  
Sheri Rynearson ◽  
Xianming Chen ◽  
Liangliang Gao ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Stripe Rust ◽  
Spring Wheat ◽  
Rust Resistance ◽  
Genome Wide Association Study ◽  
Leaf Rust Resistance ◽  
Genome Wide Association ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Genome Wide

A previous genome-wide association study (GWAS) for leaf rust (caused by Puccinia triticina) resistance identified 46 resistance quantitative trait loci (QTL) in an elite spring wheat leaf rust resistance diversity panel. With the aim of characterizing the pleiotropic resistance sources to both leaf rust and stripe rust (caused by P. striiformis f. sp. tritici), stripe rust responses were tested in five U.S. environments at the adult-plant stage and to five U.S. races at the seedling stage. The data revealed balanced phenotypic distributions in this population except for the seedling response to P. striiformis f. sp. tritici race PSTv-37. GWAS for stripe rust resistance discovered a total of 21 QTL significantly associated with all-stage or field resistance on chromosomes 1B, 1D, 2B, 3B, 4A, 5A, 5B, 5D, 6A, 6B, 7A, and 7B. Previously documented pleiotropic resistance genes Yr18/Lr34 and Yr46/Lr67 and tightly linked genes Yr17-Lr37 and Yr30-Sr2-Lr27 were also detected in this population. In addition, stripe rust resistance QTL Yrswp-2B.1, Yrswp-3B, and Yrswp-7B colocated with leaf rust resistance loci 2B_3, 3B_t2, and 7B_4, respectively. Haplotype analysis uncovered that Yrswp-3B and 3B_t2 were either tightly linked genes or the same gene for resistance to both stripe and leaf rusts. Single nucleotide polymorphism markers IWB35950, IWB74350, and IWB72134 for the 3B QTL conferring resistance to both rusts should be useful in incorporating the resistance allele(s) in new cultivars.

Genome-wide association studies revealed novel stripe rust resistance QTL in barley at seedling and adult-plant stages

Euphytica ◽  
2021 ◽  
Vol 217 (1) ◽  
Author(s):  
Sanjaya Gyawali ◽  
Sujan Mamidi ◽  
Shiaoman Chao ◽  
Subhash C. Bhardwaj ◽  
Pradeep S. Shekhawat ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Rust Resistance ◽  
Association Studies ◽  
Genome Wide Association ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Genome Wide Association Studies ◽  
Resistance Qtl ◽  
Genome Wide

scholarly journals Genome-wide association analysis of stripe rust resistance in modern Chinese wheat

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Mengjie Jia ◽  
Lijun Yang ◽  
Wei Zhang ◽  
Garry Rosewarne ◽  
Junhui Li ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Rust Resistance ◽  
Genome Wide Association ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Amplification Refractory Mutation System ◽  
Post Inoculation ◽  
Genome Wide ◽  
A Genome ◽  
Chinese Wheat

Abstract Background Stripe rust (yellow rust) is a significant disease for bread wheat (Triticum aestivum L.) worldwide. A genome-wide association study was conducted on 240 Chinese wheat cultivars and elite lines genotyped with the wheat 90 K single nucleotide polymorphism (SNP) arrays to decipher the genetic architecture of stripe rust resistance in Chinese germplasm. Results Stripe rust resistance was evaluated at the adult plant stage in Pixian and Xindu in Sichuan province in the 2015–2016 cropping season, and in Wuhan in Hubei province in the 2013–2014, 2016–2017 and 2018–2019 cropping seasons. Twelve stable loci for stripe rust resistance were identified by GWAS using TASSEL and GAPIT software. These loci were distributed on chromosomes 1B, 1D, 2A, 2B, 3A, 3B, 4B (3), 4D, 6D, and 7B and explained 3.6 to 10.3% of the phenotypic variation. Six of the loci corresponded with previously reported genes/QTLs, including Sr2/Yr30/Lr27, while the other six (QYr.hbaas-1BS, QYr.hbaas-2BL, QYr.hbaas-3AL, QYr.hbaas-4BL.3, QYr.hbaas-4DL, and QYr.hbaas-6DS) are probably novel. The results suggest high genetic diversity for stripe rust resistance in this population. The resistance alleles of QYr.hbaas-2AS, QYr.hbaas-3BS, QYr.hbaas-4DL, and QYr.hbaas-7BL were rare in the present panel, indicating their potential use in breeding for stripe rust resistance in China. Eleven penta-primer amplification refractory mutation system (PARMS) markers were developed from SNPs significantly associated with seven mapped QTLs. Twenty-seven genes were predicted for mapped QTLs. Six of them were considered as candidates for their high relative expression levels post-inoculation. Conclusion The resistant germplasm, mapped QTLs, and PARMS markers developed in this study are resources for enhancing stripe rust resistance in wheat breeding.

Characterization and genome-wide association mapping of resistance to leaf rust, stem rust and stripe rust in a geographically diverse collection of spring wheat landraces

2017 ◽  
Vol 37 (9) ◽  
Author(s):  
Peace Kankwatsa ◽  
Davinder Singh ◽  
Peter C. Thomson ◽  
Ebrahiem M. Babiker ◽  
John M. Bonman ◽  
...  
Keyword(s):  
Association Mapping ◽  
Leaf Rust ◽  
Stripe Rust ◽  
Spring Wheat ◽  
Stem Rust ◽  
Genome Wide Association ◽  
Genome Wide ◽  
Wheat Landraces

scholarly journals Genome-Wide Association Mapping of Adult-Plant Resistance to Stripe Rust in Common Wheat (Triticum aestivum)

Plant Disease ◽  
2020 ◽  
Vol 104 (8) ◽  
pp. 2174-2180 ◽  
Author(s):  
Fangping Yang ◽  
Jindong Liu ◽  
Ying Guo ◽  
Zhonghu He ◽  
Awais Rasheed ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Common Wheat ◽  
Stripe Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Genome Wide Association ◽  
Stripe Rust Resistance ◽  
Economic Losses ◽  
Genome Wide ◽  
A Genome

Stripe rust, caused by Puccinia striiformis f. sp. tritici, is a globally devastating disease of common wheat (Triticum aestivum L.), resulting in substantial economic losses. To identify effective resistance genes, a genome-wide association study was conducted on 120 common wheat lines from different wheat-growing regions of China using the wheat 90K iSelect SNP array. Seventeen loci were identified, explaining 9.5 to 21.8% of the phenotypic variation. Most of these genes were detected in the A (seven) and B (seven) genomes, with only three in the D genome. Among them, 11 loci were colocated with known resistance genes or quantitative trait loci reported previously, whereas the other six are likely new resistance loci. Annotation of flanking sequences of significantly associated SNPs indicated the presence of three important candidate genes, including E3 ubiquitin-protein ligase, F-box repeat protein, and disease resistance RPP13-like protein. This study increased our knowledge in understanding the genetic architecture for stripe rust resistance and identified wheat varieties with multiple resistance alleles, which are useful for improvement of stripe rust resistance in breeding.

scholarly journals Genome-Wide Association Studies Reveal All-Stage Rust Resistance Loci in Elite Durum Wheat Genotypes

2021 ◽  
Vol 12 ◽  
Author(s):  
Meriem Aoun ◽  
Matthew N. Rouse ◽  
James A. Kolmer ◽  
Ajay Kumar ◽  
Elias M. Elias
Keyword(s):  
Durum Wheat ◽  
Leaf Rust ◽  
Stripe Rust ◽  
Resistance Genes ◽  
Stem Rust ◽  
Rust Resistance ◽  
Genome Wide Association ◽  
Genome Wide ◽  
Rust Pathogen ◽  
Rust Resistance Genes

Leaf rust, caused by Puccinia triticina (Pt), stripe rust caused by Puccinia striiformis f. sp. tritici (Pst), and stem rust caused by Puccinia graminis f. sp. tritici (Pgt) are major diseases to wheat production globally. Host resistance is the most suitable approach to manage these fungal pathogens. We investigated the phenotypic and genotypic structure of resistance to leaf rust, stem rust, and stripe rust pathogen races at the seedling stage in a collection of advanced durum wheat breeding lines and cultivars adapted to Upper Mid-West region of the United States. Phenotypic evaluation showed that the majority of the durum wheat genotypes were susceptible to Pt isolates adapted to durum wheat, whereas all the genotypes were resistant to common wheat type-Pt isolate. The majority of genotypes were resistant to stripe rust and stem rust pathogen races. The durum panel genotyped using Illumina iSelect 90 K wheat SNP assay was used for genome-wide association mapping (GWAS). The GWAS revealed 64 marker-trait associations (MTAs) representing six leaf rust resistance loci located on chromosome arms 2AS, 2AL, 5BS, 6AL, and 6BL. Two of these loci were identified at the positions of Lr52 and Lr64 genes, whereas the remaining loci are most likely novel. A total of 46 MTAs corresponding to four loci located on chromosome arms 1BS, 5BL, and 7BL were associated with stripe rust response. None of these loci correspond to designated stripe rust resistance genes. For stem rust, a total of 260 MTAs, representing 22 loci were identified on chromosome arms 1BL, 2BL, 3AL, 3BL, 4AL, 5AL, 5BL, 6AS, 6AL, 6BL, and 7BL. Four of these loci were located at the positions of known genes/alleles (Sr7b, Sr8155B1, Sr13a, and Sr13b). The discovery of known and novel rust resistance genes and their linked SNPs will help diversify rust resistance in durum wheat.

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