scholarly journals Search for evidence of introgression of wheat (Triticum aestivum L.) traits into sea barley (Hordeum marinum s.str. Huds.) and bearded wheatgrass (Elymus caninus L.) in central and northern Europe, using isozymes, RAPD and microsatellite markers

2001 ◽  
Vol 103 (2-3) ◽  
pp. 191-196 ◽  
Author(s):  
R. Guadagnuolo ◽  
D. Savova-Bianchi ◽  
J. Keller-Senften ◽  
F. Felber
Keyword(s):  
Triticum Aestivum ◽  
Microsatellite Markers ◽  
Triticum Aestivum L ◽  
Northern Europe ◽  
Elymus Caninus

scholarly journals Molecular characterization of wheat (Triticum aestivum L.) genotypes through SSR markers

2012 ◽  
Vol 37 (3) ◽  
pp. 389-398 ◽  
Author(s):  
S Islam ◽  
MS Haque ◽  
RM Emon ◽  
MM Islam ◽  
SN Begum
Keyword(s):  
Genetic Diversity ◽  
Triticum Aestivum ◽  
Genetic Distance ◽  
Microsatellite Markers ◽  
Gene Diversity ◽  
Polymorphic Information Content ◽  
Allelic Diversity ◽  
Triticum Aestivum L ◽  
Allele Number ◽  
Average Gene

A study was undertaken to examine the genetic diversity of 12 wheat (Triticum aestivum L.) genotypes, using 4 simple sequence repeats (SSRs). A total of 10 alleles were found. Allele number per locus ranged from 2 to 4 with an average of 2.5. The polymorphic information content (PIC) values ranged from 0.2755 to 0.5411 with an average of 0.3839. The average gene diversity over all SSR loci for the 12 wheat genotypes was 0.4688, ranging from 0.3299 to 0.6042. Cluster analysis based on microsatellite allelic diversity discriminated the varieties into different clusters. Genetic diversity was the highest between variety Gourab and Akbar as well as Gourab and BAW-1064, showing a genetic distance value of 0.4697. The genetic distance was lowest between Balaka and Aghrani as well as Triticale and BAW-1036. Positive correlations were found between gene diversity, number of alleles, the allele size range and the types of repeat motif of microsatellite markers. It was found from this study that microsatellite markers could characterize and discriminate all of the genotypes. More primers should be used for saturation of different regions in further studies. Bangladesh J. Agril. Res. 37(3): 389-398, September 2012 DOI: http://dx.doi.org/10.3329/bjar.v37i3.12082

scholarly journals Molecular Assessment of Genetic Diversity among Egyptian Landraces of Wheat (Triticum aestivum L.) Using Microsatellite Markers

2020 ◽  
pp. 46-58 ◽  
Author(s):  
Ahmed Medhat Mohamed Al-Naggar ◽  
Mohamed Abd El-Maboud Abd El-Shafi ◽  
Mohamed Helmy El-Shal ◽  
Ali Hassan Anany
Keyword(s):  
Genetic Diversity ◽  
Triticum Aestivum ◽  
Microsatellite Markers ◽  
Ssr Markers ◽  
Triticum Aestivum L ◽  
Genetic Progress ◽  
High Genetic Diversity ◽  
Breeding Programs ◽  
High Polymorphism Information Content ◽  
Wheat Landraces

To increase the genetic progress in wheat (Triticum aestivum L.) yield, breeders search for germplasm of high genetic diversity, one of them is the landraces. The present study aimed at evaluating genetic diversity of 20 Egyptian wheat landraces and two cultivars using microsatellite markers (SSRs). Ten SSR markers amplified a total of 27 alleles in the set of 22 wheat accessions, of which 23 alleles (85.2%) were polymorphic. The majority of the markers showed high polymorphism information content (PIC) values (0.67-0.94), indicating the diverse nature of the wheat accessions and/or highly informative SSR markers used in this study. The genotyping data of the SSR markers were used to assess genetic variation in the wheat accessions by dendrogram. The highest genetic distance was found between G21 (Sakha 64; an Egyptian cultivar) and the landrace accession No. 9120 (G11). These two genotypes could be used as parents in a hybridization program followed by selection in the segregating generations, to identify some transgressive segregates of higher grain yield than both parents. The clustering assigned the wheat genotypes into four groups based on SSR markers. The results showed that the studied SSR markers, provided sufficient polymorphism and reproducible fingerprinting profiles for evaluating genetic diversity of wheat landraces. The analyzed wheat landraces showed a good level of genetic diversity at the molecular level. Molecular variation evaluated in this study of wheat landraces can be useful in traditional and molecular breeding programs.

scholarly journals Genetic diversity of Cameroonian bread wheat (Triticum aestivum L.) cultivars revealed by microsatellite markers

2017 ◽  
Vol 16 (36) ◽  
pp. 1832-1839 ◽  
Author(s):  
Tekeu Honore ◽  
M. L. Ngonkeu Eddy ◽  
P. Djocgoue Francois ◽  
Ellis Aletta ◽  
Lendzemo Venasius ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Triticum Aestivum ◽  
Microsatellite Markers ◽  
Bread Wheat ◽  
Triticum Aestivum L

Genetic mapping of three alleles at the Pm3 locus conferring powdery mildew resistance in common wheat (Triticum aestivum L.)

Genome ◽  
2004 ◽  
Vol 47 (6) ◽  
pp. 1130-1136 ◽  
Author(s):  
Xiu-Qiang Huang ◽  
Sai L.K Hsam ◽  
Volker Mohler ◽  
Marion S Röder ◽  
Friedrich J Zeller
Keyword(s):  
Triticum Aestivum ◽  
Powdery Mildew ◽  
Genetic Mapping ◽  
Microsatellite Markers ◽  
Microsatellite Marker ◽  
Powdery Mildew Resistance ◽  
Triticum Aestivum L ◽  
Mildew Resistance ◽  
Marker Loci ◽  
Mapping Populations

A set of differential isolates of Blumeria graminis f.sp. tritici was used to identify 10 alleles at the Pm3 locus on the short arm of chromosome 1A. Three F3 populations were used to map Pm3h in Abessi, Pm3i in line N324, and Pm3j alleles in GUS 122 relative to microsatellite markers. In total, 13 marker loci were mapped on chromosome 1AS and 1 marker on 1AL. The order of marker loci in the 3 mapping populations is consistent with previously published maps. All 3 alleles were mapped in the distal region of chromosome 1AS. The present study indicated that microsatellite markers are an ideal marker system for comparative mapping of alleles at the same gene locus in different mapping populations. The linkage distances of the closest microsatellite marker, Xgwm905–1A, to Pm3h, Pm3i, and Pm3j were 3.7 cM, 7.2 cM, and 1.2 cM, respectively. The microsatellite marker Xgwm905–1A cannot be used to distinguish between Pm3 alleles. The development of specific markers for individual Pm3 alleles is discussed on the basis of the recently cloned Pm3b allele.Key words: genetic mapping, marker-assisted selection, microsatellite markers, Pm3 locus, powdery mildew resistance, Triticum aestivum.

Assessing genetic diversity of Egyptian hexaploid wheat (Triticum aestivum L.) using microsatellite markers

2014 ◽  
Vol 62 (3) ◽  
pp. 377-385 ◽  
Author(s):  
Khaled F. M. Salem ◽  
Marion S. Röder ◽  
Andreas Börner
Keyword(s):  
Genetic Diversity ◽  
Triticum Aestivum ◽  
Microsatellite Markers ◽  
Hexaploid Wheat ◽  
Triticum Aestivum L

scholarly journals Genome-Wide Analysis of Microsatellite Markers Based on Sequenced Database in Chinese Spring Wheat (Triticum aestivum L.)

PLoS ONE ◽  
2015 ◽  
Vol 10 (11) ◽  
pp. e0141540 ◽  
Author(s):  
Bin Han ◽  
Changbiao Wang ◽  
Zhaohui Tang ◽  
Yongkang Ren ◽  
Yali Li ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Microsatellite Markers ◽  
Spring Wheat ◽  
Chinese Spring ◽  
Triticum Aestivum L ◽  
Genome Wide Analysis ◽  
Genome Wide ◽  
Chinese Spring Wheat

Assessing genetic diversity of wheat (Triticum aestivum L.) germplasm using microsatellite markers

2002 ◽  
Vol 105 (5) ◽  
pp. 699-707 ◽  
Author(s):  
X. Huang ◽  
A. Börner ◽  
M. Röder ◽  
M. Ganal
Keyword(s):  
Genetic Diversity ◽  
Triticum Aestivum ◽  
Microsatellite Markers ◽  
Triticum Aestivum L

scholarly journals Assessment of Genetic Diversity of Bread Wheat (Triticum aestivum L.) Cultivars using Microsatellite Markers

2019 ◽  
Vol 11 (29) ◽  
pp. 9-16
Author(s):  
Nasrin Ghasemi ◽  
Reza Gholi Mirfakhraii ◽  
Alireza Abbasi ◽  
◽  
◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Triticum Aestivum ◽  
Microsatellite Markers ◽  
Bread Wheat ◽  
Triticum Aestivum L

scholarly journals Genetic analysis of yield and heat stress related traits in wheat (Triticum aestivum L. em. Thell) using microsatellite markers

2015 ◽  
Vol 7 (2) ◽  
pp. 739-744 ◽  
Author(s):  
Mamta Gupta ◽  
Veena Chawla ◽  
Pankaj Garg ◽  
Neelam Yadav ◽  
Renu Munjal ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Heat Stress ◽  
Genetic Analysis ◽  
Microsatellite Markers ◽  
Threshold Value ◽  
Triticum Aestivum L ◽  
Interval Mapping ◽  
Single Marker Analysis ◽  
D Genome ◽  
A Genome

Microsatellite markers were used for genetic analysis of terminal heat tolerance in F2 (PBW373 × WH1081) population of wheat (Triticum aestivum L. em. Thell). Two parents were evaluated in field under normal sown and late sown conditions. For genotyping DNA from both parents PBW373 and WH1081 was amplified using 200 SSRs. Only 22 SSRs produced polymorphic bands, of size between 100 to 300 bp and an average of 1.45 alleles. The single marker analysis identified 19 markers indicating the putative QTLs for yield, its components and heat stress related physiological traits. The number of markers on these 16 linkage groups varied from one to four. On A genome 13 QTLs on B genome 5 QTLs and on D genome 9 QTLs were identified, respectively. The A, B and D genomes had 1360.3 cM, 272.4 cM and 919.5 cM of linkage coverage with average interval distances of 104.63 cM, 54.48 cM and 102.16 cM/Marker. A total of nine QTLs were resolved following composite interval mapping, one QTL was detected at a LOD score equal to threshold value of 2.5 while eight at LOD scores above the threshold value. All the nine QTLs were shown to be on definitive location on chromosome 3A (QDh.CCSHAU-3A, QDa.CCSHAU-3A and QPm.CCSHAU-3A), chromosome (QBm.CCSHAU-5A, QCtd.CCSHAU-5A and QCl.fl.CCSHAU-5A), chromosome6A (QPh.CCSHAU-6A) and chromosome3B (QTgw.CCSHAU and QMts.CCSHAU-3B). Use of these markers save times, resources and energy that are needed not only for raising large segregating populations for sveral generations, but also for estimating the parameters used for selection.

scholarly journals Selective recombination close to the semi-dwarfing locus through bulk-population in the hybrid between Triticum aestivum and Triticum polonicum

2019 ◽  
Vol 17 (2) ◽  
pp. 148-152
Author(s):  
Md. Anowarul Haque
Keyword(s):  
Triticum Aestivum ◽  
Microsatellite Markers ◽  
Allelic Variation ◽  
Triticum Aestivum L ◽  
Introgression Lines ◽  
Alternative Source ◽  
Dwarf Gene ◽  
Multiple Alleles ◽  
Triticum Polonicum ◽  
Reduced Height

The Rht-B1 locus on the short arm of chromosome 4B has multiple alleles that give phenotypic expressions ranging from semi-dwarfism to extreme dwarfism. Allelic variation at Rht-B1 locus was also found in semi-dwarf Triticum polonicum L. cv. IC12196. The semi-dwarf allele from IC12196 could be an alternative source of reduced height gene. The experimental lines derived from the cross between Triticum aestivum L. cv. Novosibirskaya 67 (N67) and IC12196 were analyzed concerning the introgression of semi-dwarf allele from IC12196. The aim of the present study was to assess the selection for the semi-dwarfism and recombination around the locus of semi-dwarf gene using microsatellite markers on the short arm of chromosome 4B. The Rht-IC12196 allele was allelic to Rht-B1b allele, and microsatellite markers indicated that the Rht-IC12196 allele was linked with the markers, Xbarc1045 and Xgwm113 on the short arm of chromosome 4B. Out of 112 hexaploid introgression lines, 29 lines were semi-dwarf. The Xgwm113 and Xhbg226 markers flanking the Rht-B1 locus were replaced by N67 allele in the majority of introgression lines. Although it did not intend, the region around the Rht-B1 locus was highly selectable, but on short arms of chromosome 4B, Rht-IC12196 gene near the locus Xgwm113 explained a large part of the phenotypic variation originated from N67-allele and non-parental allele in tall accessions. The Rht-B1a allele was favorable in the consequence of bulk population breeding and the frequency of Rht-B1a allele was 73% in the hexaploid introgression lines. J. Bangladesh Agril. Univ. 17(2): 148–152, June 2019

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