Phenotypic analysis of Salmonella enterica serovar Typhimurium rpoE mutants encoding RNA polymerase extracytoplasmic stress response sigma factors σE with altered promoter specificity

ABSTRACT Promoter-lux fusions that showed rifampin-modulated transcription were identified from a Salmonella enterica serovar Typhimurium 14028 reporter library. The transformation of a subset of fusions into mutants that lacked one of six global regulatory proteins or were rifampin resistant showed that transcription modulation was independent of the global regulators, promoter specific, and dependent on the interaction of rifampin with RNA polymerase.

Download Full-text

σ s-Dependent carbon-starvation induction of pbpG (PBP 7) is required for the starvation-stress response in Salmonella enterica serovar Typhimurium

Microbiology ◽

10.1099/mic.0.2007/005199-0 ◽

2007 ◽

Vol 153 (7) ◽

pp. 2148-2158 ◽

Cited By ~ 9

Author(s):

William J Kenyon ◽

Kristy L Nicholson ◽

Bronislava Rezuchova ◽

Dagmar Homerova ◽

Francisco Garcia-del Portillo ◽

...

Keyword(s):

Stress Response ◽

Salmonella Enterica ◽

Salmonella Enterica Serovar Typhimurium ◽

Carbon Starvation ◽

Starvation Stress ◽

Serovar Typhimurium

Download Full-text

ChIP-Seq Analysis of the σE Regulon of Salmonella enterica Serovar Typhimurium Reveals New Genes Implicated in Heat Shock and Oxidative Stress Response

PLoS ONE ◽

10.1371/journal.pone.0138466 ◽

2015 ◽

Vol 10 (9) ◽

pp. e0138466 ◽

Cited By ~ 14

Author(s):

Jie Li ◽

Christopher C. Overall ◽

Rudd C. Johnson ◽

Marcus B. Jones ◽

Jason E. McDermott ◽

...

Keyword(s):

Oxidative Stress ◽

Stress Response ◽

Heat Shock ◽

Salmonella Enterica ◽

Salmonella Enterica Serovar Typhimurium ◽

Oxidative Stress Response ◽

New Genes ◽

Serovar Typhimurium ◽

And Oxidative Stress

Download Full-text

Periplasmic peptidyl-prolyl isomerases SurA and FkpA play an important role in the starvation-stress response (SSR) of Salmonella enterica serovar Typhimurium

Antonie van Leeuwenhoek ◽

10.1007/s10482-010-9428-2 ◽

2010 ◽

Vol 98 (1) ◽

pp. 51-63 ◽

Cited By ~ 8

Author(s):

William J. Kenyon ◽

Sue Humphreys ◽

Mark Roberts ◽

Michael P. Spector

Keyword(s):

Stress Response ◽

Salmonella Enterica ◽

Salmonella Enterica Serovar Typhimurium ◽

Starvation Stress ◽

Serovar Typhimurium ◽

Peptidyl Prolyl Isomerases

Download Full-text

FliT Acts as an Anti-FlhD2C2 Factor in the Transcriptional Control of the Flagellar Regulon in Salmonella enterica Serovar Typhimurium

Journal of Bacteriology ◽

10.1128/jb.00799-06 ◽

2006 ◽

Vol 188 (18) ◽

pp. 6703-6708 ◽

Cited By ~ 70

Author(s):

Shouji Yamamoto ◽

Kazuhiro Kutsukake

Keyword(s):

Rna Polymerase ◽

Western Blotting ◽

Salmonella Enterica ◽

Transcriptional Control ◽

Salmonella Enterica Serovar Typhimurium ◽

Flagellar Gene ◽

Gene Products ◽

Serovar Typhimurium ◽

Class 1 ◽

Flagellar Regulon

ABSTRACT Flagellar operons are divided into three classes with respect to their transcriptional hierarchy in Salmonella enterica serovar Typhimurium. The class 1 gene products FlhD and FlhC act together in an FlhD2C2 heterotetramer, which binds upstream of the class 2 promoters to facilitate binding of RNA polymerase. Class 2 expression is known to be enhanced by a disruption mutation in a flagellar gene, fliT. In this study, we purified FliT protein in a His-tagged form and showed that the protein prevented binding of FlhD2C2 to the class 2 promoter and inhibited FlhD2C2-dependent transcription. Pull-down and far-Western blotting analyses revealed that the FliT protein was capable of binding to FlhD2C2 and FlhC and not to FlhD alone. We conclude that FliT acts as an anti-FlhD2C2 factor, which binds to FlhD2C2 through interaction with the FlhC subunit and inhibits its binding to the class 2 promoter.

Download Full-text