Trace metals in mussel shells and corresponding soft tissue samples: a validation experiment for the use of Perna perna shells in pollution monitoring

It is widely recognised that, unless special steps are taken, analytical results from a group of laboratories engaged in a monitoring programme are likely to be of poor comparability. This in turn can prejudice the conclusions drawn from the results of monitoring. On the basis of previous studies, the problem is known to be particularly acute for measurements of trace metals in saline waters. Recognising the difficulty, the Marine Pollution Monitoring Management Group (MPMMG) and the Water Research centre (WRc) have organised a programme of Analytical Quality Control (AQC). This has the objective of ensuring that analytical results for filterable cadmium and mercury in saline waters, obtained by water industry and other relevant laboratories, are of adequate accuracy and comparability for their intended uses. WRc is to coordinate a series of tests, some involving distributions of standards and samples, which the participating laboratories undertake; this series of tests, the background to the approach and some of the results obtained to date are described here.

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Canine Synovial Sarcoma of the Stifle Joint with Pulmonary and Liver Metastases

Tanzania Veterinary Journal ◽

10.4314/tvj.v38i1.2s ◽

2021 ◽

Vol 38 ◽

pp. 6-12

Author(s):

R.J. Samson ◽

F.H. Mpagike ◽

A.K. Felix ◽

A.B. Matondo ◽

M. Makungu

Keyword(s):

Soft Tissue ◽

Lung Metastases ◽

Synovial Cell ◽

Proximal Region ◽

Radiographic Examination ◽

Tissue Samples ◽

Stifle Joint ◽

Mongrel Dog ◽

Biopsy Examination ◽

The Right

A seven-year-old female mongrel dog was presented at the Sokoine University of Agriculture Teaching Animal Hospital for second opinion regarding a progressive swelling of the right hind limb of two months duration. Clinical examination revealed a loss of body condition, tachypnea and tachycardia, a painful immobile solid mass of 20 cm x 14 cm x 10 cm located on the right stifle joint, leucocytosis, and anaemia. Radiographic examination of the joint revealed marked soft tissue swelling with amorphous areas of mineralization and complete destruction of the proximal tibia. Smooth and solid periosteal reaction was seen around the tibia and fibula with a Codman’s triangle. Multiple nodules and a mass with soft tissue opacity were seen in the lung fields. Post-mortem examination revealed separation of tibia and fibula, softening of the proximal parts of the tibia, and complete integration of the proximal region of the two bones and articular tissues into the surrounding muscular tissues. Multifocal nodular lesions of variable sizes were mostly found in the lungs and partly in the liver. Histologically, predominantly oval and round cells with variable nuclear sizes and moderate mitoses were observed in tissue samples from the joint, lungs and the liver although some parts of the lung metastases showed both round and spindle shaped tumor cells. Clinical profile is suggestive of aggressive biphasic (spindle and epithelial) type of synovial cell sarcoma with lung and liver metastasis. Early radiographic and biopsy examination of persistent musculoskeletal nodules is recommended for early diagnosis and interventions.

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Distribution and Association of Trace Metals in Soft Tissue and Byssus of Mytilus edulis from the East Coast of Kyushu Island, Japan

Archives of Environmental Contamination and Toxicology ◽

10.1007/s002449900173 ◽

1997 ◽

Vol 32 (2) ◽

pp. 184-190 ◽

Cited By ~ 32

Author(s):

P. Szefer ◽

K. Ikuta ◽

S. Kushiyama ◽

K. Szefer ◽

K. Frelek ◽

...

Keyword(s):

Trace Metals ◽

Soft Tissue ◽

Mytilus Edulis ◽

East Coast ◽

Kyushu Island

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Kinetic Modelling of [68Ga]Ga-DOTA-Siglec-9 in Porcine Osteomyelitis and Soft Tissue Infections

Molecules ◽

10.3390/molecules24224094 ◽

2019 ◽

Vol 24 (22) ◽

pp. 4094 ◽

Cited By ~ 2

Author(s):

Lars Jødal ◽

Anne Roivainen ◽

Vesa Oikonen ◽

Sirpa Jalkanen ◽

Søren B. Hansen ◽

...

Keyword(s):

Soft Tissue ◽

Hind Limb ◽

Kinetic Modelling ◽

Compartment Model ◽

Cell Surface Expression ◽

Soft Tissue Infections ◽

Tissue Samples ◽

Tissue Compartment ◽

Arterial Blood ◽

Kinetics Of

Background: [68Ga]Ga-DOTA-Siglec-9 is a positron emission tomography (PET) radioligand for vascular adhesion protein 1 (VAP-1), a protein involved in leukocyte trafficking. The tracer facilitates the imaging of inflammation and infection. Here, we studied the pharmacokinetic modelling of [68Ga]Ga-DOTA-Siglec-9 in osteomyelitis and soft tissue infections in pigs. Methods: Eight pigs with osteomyelitis and soft tissue infections in the right hind limb were dynamically PET scanned for 60 min along with arterial blood sampling. The fraction of radioactivity in the blood accounted for by the parent tracer was evaluated with radio-high-performance liquid chromatography. One- and two-tissue compartment models were used for pharmacokinetic evaluation. Post-mortem soft tissue samples from one pig were analysed with anti-VAP-1 immunofluorescence. In each analysis, the animal’s non-infected left hind limb was used as a control. Results: Tracer uptake was elevated in soft tissue infections but remained low in osteomyelitis. The kinetics of [68Ga]Ga-DOTA-Siglec-9 followed a reversible 2-tissue compartment model. The tracer metabolized quickly; however, taking this into account, produced more ambiguous results. Infected soft tissue samples showed endothelial cell surface expression of the Siglec-9 receptor VAP-1. Conclusion: The kinetics of [68Ga]Ga-DOTA-Siglec-9 uptake in porcine soft tissue infections are best described by the 2-tissue compartment model.

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3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography

Journal of Visualized Experiments ◽

10.3791/60251 ◽

2019 ◽

Author(s):

Madleen Busse ◽

Mark Müller ◽

Melanie A. Kimm ◽

Simone Ferstl ◽

Sebastian Allner ◽

...

Keyword(s):

Computed Tomography ◽

Soft Tissue ◽

3D Imaging ◽

Staining Method ◽

Specific Staining ◽

Tissue Samples ◽

X Ray

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X-ray Fluorescence Analysis of Trace Metals in the Annual Growth Layers of Freshwater Mussel Shells

Advances in X-ray Analysis ◽

10.1154/s0376030800020140 ◽

1989 ◽

Vol 33 ◽

pp. 665-670

Author(s):

William E. Maddox ◽

Leon Duobinis-Gray ◽

David A. Owen ◽

James B. Sickel

Keyword(s):

Trace Metals ◽

Metal Pollution ◽

Freshwater Mussels ◽

Freshwater Mussel ◽

Fluorescence Analysis ◽

Annual Growth ◽

X Ray ◽

Filter Feeders ◽

Mussel Shells

Freshwater mussels (Mollusca: Unionidae) are filter feeders that are relatively immobile, widely distributed and are known to concentrate trace metals in their shells (1,2,3). These characteristics make them good candidates for monitoring metal pollution in lakes and rivers. Another characteristic of mussels that make them particularly attractive as pollution monitors is the fact the shell is deposited in distinctive, annual growth layers. The concentrations of metals in these shell layers may provide a history ol the metals present in the environment where the mussel was collected.

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