Residue analysis of glucocorticoids in bovine milk by liquid chromatography–tandem mass spectrometry

2010 ◽  
Vol 397 (6) ◽  
pp. 2477-2490 ◽  
Author(s):  
Fulvia Caretti ◽  
Alessandra Gentili ◽  
Annalisa Ambrosi ◽  
Lucia Mainero Rocca ◽  
Maurizio Delfini ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Residue Analysis ◽  
Bovine Milk ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

Application of Enzyme Digestion and Deconjugation Followed by Quick, Easy, Cheap, Effective, Rugged, Safe Extraction and Liquid Chromatography–Tandem Mass Spectrometry Methodology To Determine Ractopamine Residue in Pork

2018 ◽  
Vol 81 (8) ◽  
pp. 1258-1263 ◽  
Author(s):  
VANESSA GRESSLER ◽  
VIVIAN FEDDERN ◽  
JANE de OLIVEIRA PEIXOTO ◽  
MONICA CORREA LEDUR ◽  
OSMAR ANTONIO DALLA COSTA ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Sequential Analysis ◽  
Limit Of Detection ◽  
Residue Analysis ◽  
Tandem Mass ◽  
Method Performance ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

ABSTRACT A new methodology is proposed for ractopamine residue analysis in pork. It consists of enzyme-mediated digestion and deconjugation steps; modified quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction; and liquid chromatography–tandem mass spectrometry (LC-MS/MS). In brief, the samples were digested with protease and then deconjugated with β-glucuronidase enzyme; they were then subjected to extraction and cleanup by QuEChERS and underwent sequential analysis by LC-MS/MS. The method performance was evaluated in accordance to the validation guidelines regulated by the Brazilian Ministry of Agriculture and Food Supply. The limit of detection was 0.15 μg/kg and limit of quantification was 0.5 μg/kg. When the method was applied to real samples, ractopamine residue was found in concentrations (up to 7.86 μg/kg) below international recommendation limits up to 10 μg/kg. The method is sensitive, accurate, quick, simple, and suitable for routine analysis; therefore, it is a monitoring tool that may be adopted by laboratories to achieve compliance levels.

Sign in / Sign up

Export Citation Format

Share Document