Upgrading bioluminescent bacterial bioreporter performance by splitting the lux operon

2010 ◽  
Vol 400 (4) ◽  
pp. 1071-1082 ◽  
Author(s):  
Sharon Yagur-Kroll ◽  
Shimshon Belkin
Keyword(s):  
Lux Operon

Sequence of the luxD gene encoding acyltransferase of the lux operon from Photobacterium leiognathi

Gene ◽  
1993 ◽  
Vol 126 (1) ◽  
pp. 155-156 ◽  
Author(s):  
Chao Yuh-Fen ◽  
Weng Shu-Fen ◽  
Lin Juey-Wen
Keyword(s):  
Gene Encoding ◽  
Photobacterium Leiognathi ◽  
Lux Operon

scholarly journals Diversity and evolution of bacterial bioluminescence genes in the global ocean

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Thomas Vannier ◽  
Pascal Hingamp ◽  
Floriane Turrel ◽  
Lisa Tanet ◽  
Magali Lescot ◽  
...  
Keyword(s):  
Light Emission ◽  
Global Ocean ◽  
Light Emitting ◽  
Planetary Scale ◽  
Bacterial Bioluminescence ◽  
Lux Operon ◽  
History Of ◽  
Operon Evolution ◽  
Operon Organization ◽  
Bacterial Genes

Abstract Although bioluminescent bacteria are the most abundant and widely distributed of all light-emitting organisms, the biological role and evolutionary history of bacterial luminescence are still shrouded in mystery. Bioluminescence has so far been observed in the genomes of three families of Gammaproteobacteria in the form of canonical lux operons that adopt the CDAB(F)E(G) gene order. LuxA and luxB encode the two subunits of bacterial luciferase responsible for light-emission. Our deep exploration of public marine environmental databases considerably expands this view by providing a catalog of new lux homolog sequences, including 401 previously unknown luciferase-related genes. It also reveals a broader diversity of the lux operon organization, which we observed in previously undescribed configurations such as CEDA, CAED and AxxCE. This expanded operon diversity provides clues for deciphering lux operon evolution and propagation within the bacterial domain. Leveraging quantitative tracking of marine bacterial genes afforded by planetary scale metagenomic sampling, our study also reveals that the novel lux genes and operons described herein are more abundant in the global ocean than the canonical CDAB(F)E(G) operon.

scholarly journals Colonization of Host Plants by the Fire Blight Pathogen Erwinia amylovora Marked with Genes for Bioluminescence and Fluorescence

1998 ◽  
Vol 88 (5) ◽  
pp. 416-421 ◽  
Author(s):  
Jochen Bogs ◽  
Iris Bruchmüller ◽  
Claudia Erbar ◽  
Klaus Geider
Keyword(s):  
Host Plants ◽  
Fire Blight ◽  
Erwinia Amylovora ◽  
Vibrio Fischeri ◽  
Bacterial Invasion ◽  
Leaf Hairs ◽  
Lux Operon ◽  
And Migration ◽  
Green Fluorescent ◽  
Lac Promoter

To follow the movement of Erwinia amylovora in plant tissue without dissection, this bacterium was marked with either the lux operon from Vibrio fischeri or the gfp gene from the jellyfish Aequorea victoria, both carried on multicopy plasmids and expressed under the control of the lac promoter from Escherichia coli. Movement of the pathogen was visualized in leaves, stems, and roots of apple seedlings, and migration of E. amylovora was traced from inoculation sites in the stem to as far as the roots. Green fluorescent E. amylovora cells were observed in the xylem and later appeared to break out of the vessels into the intercellular spaces of the adjacent parenchyma. Inoculation in the intercostal region of leaves caused a zone of slow necrosis that finally resulted in bacterial invasion of the xylem vessels. Labeled bacteria could also be seen in association with the anchor sites of leaf hairs. Distortion of the epidermis adjacent to leaf hairs created openings that were observed by scanning electron microscopy. As the intercostal region, the bases of leaf hairs provided E. amylovora access to intact xylem vessels, which allowed further distribution of the pathogen in the host plant.

The lumazine protein-encoding gene in Photobacterium leiognathi is linked to the lux operon

Gene ◽  
1993 ◽  
Vol 126 (1) ◽  
pp. 153-154 ◽  
Author(s):  
Juey-Wen Lin ◽  
Yuh-Fen Chao ◽  
Shu-Fen Weng
Keyword(s):  
Photobacterium Leiognathi ◽  
Lux Operon ◽  
Protein Encoding ◽  
Encoding Gene

scholarly journals A new Vibrio fischeri lux gene precedes a bidirectional termination site for the lux operon.

1990 ◽  
Vol 172 (12) ◽  
pp. 6797-6802 ◽  
Author(s):  
A Swartzman ◽  
S Kapoor ◽  
A F Graham ◽  
E A Meighen
Keyword(s):  
Vibrio Fischeri ◽  
Lux Operon

Involvement of host factors in the regulation of the Vibrio fischeri lux operon in Escherichia coli cells

Microbiology ◽  
2006 ◽  
Vol 75 (4) ◽  
pp. 452-458 ◽  
Author(s):  
I. V. Manukhov ◽  
V. Yu. Kotova ◽  
G. B. Zavil’gel’sky
Keyword(s):  
Escherichia Coli ◽  
Vibrio Fischeri ◽  
Host Factors ◽  
Escherichia Coli Cells ◽  
Lux Operon

Proximal and distal sites bind LuxR independently and activate expression of the Vibrio harveyi lux operon

1994 ◽  
Vol 14 (2) ◽  
pp. 255-262 ◽  
Author(s):  
Carol M. Miyamoto ◽  
Eric E. Smith ◽  
Eiana Swartzman ◽  
Jie‐Gang Cao ◽  
Angus F. Graham ◽  
...  
Keyword(s):  
Vibrio Harveyi ◽  
Lux Operon

scholarly journals Bioluminescence as a tool in molecular biology

2017 ◽  
Vol 71 (0) ◽  
pp. 0-0
Author(s):  
Katarzyna Pajor ◽  
Daniel Sypniewski ◽  
Ilona Bednarek
Keyword(s):  
Molecular Biology ◽  
Protein Interactions ◽  
Bioluminescence Imaging ◽  
Light Emission ◽  
Protein Protein Interactions ◽  
Photinus Pyralis ◽  
Bacterial Bioluminescence ◽  
Lux Operon

Bioluminescence has been studied for many years by scientists. There are numerous mechanisms of that phenomenon; among them bacterial bioluminescence is the most frequently found in nature. This type of bioluminescence is determined by the appearance of lux operon, which encodes all elements necessary to produce light emission and it does not require any additional substrates supply. Another commonly found example of bioluminescence mechanism is performed by Photinus pyralis. Luciferase of P. pyralis named FLuc requires D-luciferin as a substrate. Bioluminescence is also characteristic for many deep-sea organisms. Most of them are based on oxidation reaction of coelenterazine to coelenteramide mediated by RLuc or GLuc luciferases. Due to the variety of bioluminescence mechanisms in nature, it has become possible to apply them in many sensitive methods that can be used in molecular biology and medicine. The most significant application of bioluminescence is BLI (bioluminescence imaging). This method is cheap and nontoxic which allows both in vitro and in vivo imaging. BLI applications include, e.g. protein-protein interactions, stem cells labeling, tracking of viral, bacterial, fungal and parasitical infections, and carcinogenesis analyses. Bioluminescence has also been used in the creation of modified cell systems capable of light emission in response to certain analytes and thus very sensitive biosensors have been generated. Other important areas of bioluminescence application are immunoassays, ATP assays, and BART analysis (bioluminescent assay in Real-Time) – a very sensitive technique which allows scientists to estimate nucleic acids amplification.

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