Biosynthesis of 15N-labeled cylindrospermopsin and its application as internal standard in stable isotope dilution analysis

2014 ◽  
Vol 406 (24) ◽  
pp. 5765-5774 ◽  
Author(s):  
Katrin Kittler ◽  
Holger Hoffmann ◽  
Franziska Lindemann ◽  
Matthias Koch ◽  
Sascha Rohn ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Isotope Dilution ◽  
Internal Standard ◽  
Isotope Dilution Analysis ◽  
Stable Isotope Dilution Analysis ◽  
Stable Isotope Dilution

Preparation of 13C-labelled cis-zearalenone and its application as internal standard in stable isotope dilution analysis

2014 ◽  
Vol 7 (1) ◽  
pp. 45-52 ◽  
Author(s):  
S. Drzymala ◽  
J. Riedel ◽  
R. Köppen ◽  
L.-A. Garbe ◽  
M. Koch
Keyword(s):  
Stable Isotope ◽  
Isotope Dilution ◽  
Edible Oils ◽  
Uv Light ◽  
Internal Standard ◽  
Edible Oil ◽  
Isotope Dilution Analysis ◽  
Stable Isotope Dilution Analysis ◽  
Stable Isotope Dilution ◽  
Maize Germ

Pure U-[13C18]-labelled cis-zearalenone (cis-ZEA) has been prepared and characterised as internal standard (ISTD) for a reliable quantification of cis-ZEA in contaminated food and feed products. The cis-isomer of the naturally trans-configurated Fusarium mycotoxin zearalenone is often neglected. However, isomerisation easily occurs by exposure of ZEA to (UV-)light. Thus, the applicability of the new cis-ZEA ISTD was demonstrated in a long-term isomerisation study comparing naturally trans-ZEA-contaminated edible oil with spiked edible oil. To estimate the benefits of the newly prepared cis-ZEA ISTD, various approaches to quantify cis-ZEA by high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) were compared. As a result, a significant bias was revealed if no appropriate cis-ZEA standards are used. Furthermore, the new ISTD was applied to the analysis of 15 edible oils by stable isotope dilution analysis in combination with HPLC-electrospray ionisation-MS/MS. One of the maize germ oils showed the presence of cis-ZEA above LOD (≯0.3 μg/kg), whereas two out of 15 maize germ oils were found to be contaminated with trans-ZEA (range 17.0-31.0 μg/kg).

scholarly journals A Novel Method for the Quantification of Quinic Acid in Food Using Stable Isotope Dilution Analysis

2009 ◽  
Vol 92 (3) ◽  
pp. 730-733 ◽  
Author(s):  
Thomas Erk ◽  
Hannah Bergmann ◽  
Elke Richling
Keyword(s):  
Stable Isotope ◽  
Isotope Dilution ◽  
Quinic Acid ◽  
Isotope Dilution Analysis ◽  
Red Wines ◽  
Stable Isotope Dilution Analysis ◽  
Selective Method ◽  
Stable Isotope Dilution ◽  
Novel Method ◽  
First Time

Abstract Organic acids play an important role in the flavor and taste of plant-derived foods. Quinic acid (QA) is one of the major acids. In the past, several methods like HPLC/UV, GC, and capillary electrophoresis were used for identification and quantification of QA. For the first time, a novel, sensitive, and selective method for the quantification of QA in food using stable isotope dilution analysis with HPLC/MS/MS has been established. Uniformly labeled 13C-QA was used as a standard to reduce sample preparations and to overcome matrix and ionization effects. The method was used to determine the QA content of red wines, instant coffees, and cloudy apple juices. QA contents of instant coffees were 64.4 and 63.6 g/kg powder. The concentrations in red wines were 24.0 and 25.1 mg/L, and 1493.3 and 1705.2 mg/L in cloudy apple juices.

scholarly journals Quantitative Analysis of 2-Aminoacetophenone in Off-Flavored Wines by Stable Isotope Dilution Assay

1996 ◽  
Vol 79 (2) ◽  
pp. 583-586 ◽  
Author(s):  
Bettina Dollmann ◽  
Dominicus Wichmann ◽  
Alfred Schmitt ◽  
Hans Koehler ◽  
Peter Schreier
Keyword(s):  
Quantitative Analysis ◽  
Stable Isotope ◽  
Isotope Dilution ◽  
Internal Standard ◽  
Stable Isotope Dilution Assay ◽  
Isotope Dilution Analysis ◽  
Model Experiments ◽  
Stable Isotope Dilution ◽  
Isotope Dilution Assay ◽  
Dilution Assay

Abstract Isotope dilution analysis was used to quantitate 2- aminoacetophenone in wines exhibiting the so- called untypical aging off-flavor. d3-Aminoacetophe- none was synthesized and used as isotopomeric internal standard. The method of quantitation was verified by several model experiments. In the off-flavored wines studied, amounts of 2-aminoacetophe none ranging from 0.7 to 12.8 μg/L were determined.

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