A new method for the determination of peak distribution across a two-dimensional separation space for the identification of optimal column combinations

The spectrophotometric titration of three-step sequential dissociation equilibria is analyzed by means of two dimensional absorbance diagrams. The evaluation of pK1 and pK3 as well as of the absorbance coefficients of both ampholytes is made by the uniform segments at the beginning and end of the titration. New methods of evaluating the value of pK2 precisely and without difficulty even at small pK-differences are described. By means of several independent criteria the pK-values and the absorbance coefficients of the ampholytes can be examined. The titration of (1,2,4)-benzenetricarboxylic acid illustrates the new method.

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A new method for the determination of nitrates

Analytica Chimica Acta ◽

10.1016/s0003-2670(01)81308-8 ◽

1960 ◽

Vol 23 ◽

pp. 227-232 ◽

Cited By ~ 2

Author(s):

P WEST ◽

G LYLES

Keyword(s):

New Method

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A new method for non-invasive, manoeuvre-free determination of “static” pressure–volume curves during dynamic/therapeutic mechanical ventilation

Acta Anaesthesiologica Scandinavica ◽

10.1034/j.1399-6576.2000.00516.x ◽

2000 ◽

Vol 44 (5) ◽

pp. 578 ◽

Cited By ~ 4

Author(s):

S. Kárason

Keyword(s):

Mechanical Ventilation ◽

Static Pressure ◽

New Method ◽

Non Invasive

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A New Method for the Determination of Plasma Activators of Fibrinolysis

Thrombosis and Haemostasis ◽

10.1055/s-0038-1649221 ◽

1977 ◽

Vol 37 (02) ◽

pp. 210-215 ◽

Cited By ~ 1

Author(s):

R Margalit ◽

E Gidron ◽

Y Shalitin

Keyword(s):

New Method ◽

Effective Activator

SummaryThe term “effective activator” of plasminogen is proposed, to denote the resultant of activator-antiactivator interaction, and a method for the determination of the level of these activators is described. By adding axcess plasminogen to the euglobulin fraction of plasma the influence of the level of endogenous plasminogen and of the antiplasmin is eliminated. It is shown that the level of fibrinogen has very little bearing on the results. An effective activator unit is defined as equal to 1 CTA unit of urokinase activity on a fibrinogen-plasminogen substrate.

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The Plasmin Inhibitors of Plasma

Thrombosis and Haemostasis ◽

10.1055/s-0038-1655579 ◽

1964 ◽

Vol 12 (01) ◽

pp. 119-125 ◽

Cited By ~ 9

Author(s):

Y Shamash ◽

A Rimon

Keyword(s):

Human Plasma ◽

New Method ◽

Normal Amount ◽

Caseinolytic Activity ◽

Before And After ◽

Plasmin Inhibitors

SummaryA new method for the assay of plasmin inhibitors in human plasma is described. The method consists of determination of the caseinolytic activity of a standard plasmin solution before and after incubation with the inhibitor, with lysine added to the mixture as a stabilizer of plasmin. Using this method, it was found that plasma contains enough inhibitors to inactivate 30 caseinolytic units of plasmin, or 10 times the normal amount of plasminogen in human plasma.

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AGGLUTINATION INHIBITION REACTIONS FOR THE DETERMINATION OF GONADOTROPHINS

Acta Endocrinologica ◽

10.1530/acta.0.062s095 ◽

1969 ◽

Vol 62 (1_Suppl) ◽

pp. S95-S112 ◽

Cited By ~ 3

Author(s):

A. H. W. M. Schuurs

Keyword(s):

Immune System ◽

Quantitative Determination ◽

Haemagglutination Inhibition ◽

Latex Agglutination ◽

Literature Survey ◽

New Method ◽

Test Fluid ◽

Main Application ◽

Test Systems

ABSTRACT Various techniques for sensitising erythrocytes and latex particles with gonadotrophins, particularly with HCG, are described. The haemagglutination inhibition reactions are generally interpreted by means of »erythrocyte settling patterns«. By a new method of evaluating these patterns a relatively precise quantitative determination is possible. Latex agglutination inhibition reactions on slides are particularly suitable as rapid qualitative tests. In cases where the maximum attainable sensitivity of the agglutination inhibition tests is insufficient, e. g. for determining LH concentrations in urine, the hormone in the test fluid has to be concentrated or extracted. An alternative method is a modified haemagglutination inhibition test for large volumes which is applicable to unconcentrated urine. Due to non-specific inhibitions the above-mentioned tests cannot be applied to unprocessed serum. Agglutination inhibition tests with HCG are already well advanced, pregnancy diagnosis being their main application. Now that highly purified HCG is available, a satisfactory specificity for these tests can be attained. If the immune system for HCG is used for estimating LH, it has to meet additional specificity requirements. Furthermore, the measure of cross-reaction and the choice of standard merit special attention. Finally, a literature survey is given of test systems in which LH and FSH were used as antigens.

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