Genetically encoded FRET fluorescent sensor designed for detecting MOF histone acetyltransferase activity in vitro and in living cells

2021 ◽  
Author(s):  
Qianqian Han ◽  
Feng Chen ◽  
Shushan Liu ◽  
Yushu Ge ◽  
Jiang Wu ◽  
...  
Keyword(s):  
Histone Acetyltransferase ◽  
Fluorescent Sensor ◽  
Living Cells ◽  
Acetyltransferase Activity ◽  
Histone Acetyltransferase Activity

In vitro analysis of histone acetyltransferase activity

Methods ◽  
2004 ◽  
Vol 33 (1) ◽  
pp. 45-52 ◽  
Author(s):  
Laura J Benson ◽  
Anthony T Annunziato
Keyword(s):  
Histone Acetyltransferase ◽  
Acetyltransferase Activity ◽  
Vitro Analysis ◽  
In Vitro Analysis ◽  
Histone Acetyltransferase Activity

scholarly journals Human TFIIIC Relieves Chromatin-Mediated Repression of RNA Polymerase III Transcription and Contains an Intrinsic Histone Acetyltransferase Activity

1999 ◽  
Vol 19 (2) ◽  
pp. 1605-1615 ◽  
Author(s):  
Tapas K. Kundu ◽  
Zhengxin Wang ◽  
Robert G. Roeder
Keyword(s):  
Rna Polymerase ◽  
Histone Acetyltransferase ◽  
Rna Polymerase Iii ◽  
Acetyltransferase Activity ◽  
Naked Dna ◽  
Polymerase Iii ◽  
Chromatin Template ◽  
Direct Recognition ◽  
Histone Acetyltransferase Activity

ABSTRACT Human TFIIIC is a multisubunit factor that is essential for transcription by RNA polymerase III on tRNA and virus-associated RNA genes and initiates preinitiation complex assembly by direct recognition of promoter elements. We show that highly purified TFIIIC, at concentrations above those sufficient for transcription of naked DNA templates, effectively relieves nucleosome-mediated repression on an in vitro-reconstituted chromatin template. Highly purified TFIIIC alone can bind to the A and B boxes of a tRNA gene within a chromatin template and, further, displays a histone acetyltransferase activity that is intrinsic to at least one (and probably three) of its subunits. The possibility of a direct link between TFIIIC-dependent chromatin transcription and acetyltransferase activities is suggested by the partial loss of these activities, but not DNA transcription activity, following pretreatment of TFIIIC withp-hydroxymercuribenzoic acid.

Assays of nucleosome assembly and the inhibition of histone acetyltransferase activity. (5) Preparation of core histones

2007 ◽  
Author(s):  
Takahito Yamasaki ◽  
Takehide Murata ◽  
Chunyuan Jin ◽  
Kohsuke Kato ◽  
Michiya Noguchi ◽  
...  
Keyword(s):  
Histone Acetyltransferase ◽  
Nucleosome Assembly ◽  
Acetyltransferase Activity ◽  
Core Histones ◽  
Histone Acetyltransferase Activity

Ethanol Extract of Capsella bursa-pastoris Improves Hepatic Steatosis Through Inhibition of Histone Acetyltransferase Activity

2017 ◽  
Vol 20 (3) ◽  
pp. 251-257 ◽  
Author(s):  
Hyo-Kyoung Choi ◽  
Eun Ju Shin ◽  
Su Jin Park ◽  
Haeng Jeon Hur ◽  
Jae Ho Park ◽  
...  
Keyword(s):  
Hepatic Steatosis ◽  
Histone Acetyltransferase ◽  
Ethanol Extract ◽  
Acetyltransferase Activity ◽  
Histone Acetyltransferase Activity
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