Quantification of active selenols in cells: a selenol-specific recognition europium-switched signal-amplification ICP-MS approach

2021 ◽  
Author(s):  
Jiaxuan Zhang ◽  
Yang Zhou ◽  
Dongliang Zuo ◽  
Limin Yang ◽  
Xiaowen Yan ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Specific Recognition ◽  
Icp Ms ◽  
In Cells

A Novel Inductive Coupled Plasma Mass Spectrometry Gene Detection Method Based on AuNPs and Bio-Barcode Signal Amplification

2019 ◽  
Vol 11 (5) ◽  
pp. 638-644 ◽  
Author(s):  
Pengfei Jiang ◽  
Chomba Haji ◽  
Xiufang Ye ◽  
Menglei Chang ◽  
Wenjun Li ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Signal Amplification ◽  
Limit Of Detection ◽  
High Sensitivity ◽  
Mismatched Dna ◽  
Icp Ms ◽  
Target Dna ◽  
Cancer Related Gene ◽  
Plasma Mass Spectrometry ◽  
Double Base

An inductive coupled plasma mass spectrometry (ICP-MS) method based on gold nanoparticles (AuNPs) and bio-barcode signal amplification was presented for the sensitive detection of gastric cancer related gene. The target DNA, magnetic nanoprobes and SiO2/AuNPs barcode probes were hybridized to form a "sandwich" structure, then a large amount of AuNPs which were amplified by bio-barcode technology were detected by ICP-MS. This method presented a limit of detection as low as 1 fM. The ratio of the background-subtracted 197 Au signals for totally complementary DNA, single-base mismatched DNA, double-base mismatched DNA and totally mismatched DNA was 115:30:16:1, respectively. It's suggested that the complementary and mismatched DNA can be distinguished clearly. This novel ICP-MS biosensor is prospective in DNA detection with high sensitivity and specificity.

Chip-based monolithic microextraction combined with ICP-MS for the determination of bismuth in HepG2 cells

2016 ◽  
Vol 31 (7) ◽  
pp. 1391-1399 ◽  
Author(s):  
Jing Zhang ◽  
Beibei Chen ◽  
Han Wang ◽  
Xi Huang ◽  
Man He ◽  
...  
Keyword(s):  
Trace Metals ◽  
Hepg2 Cells ◽  
Icp Ms ◽  
New Strategy ◽  
In Cells

Chip-based monolithic microextraction combined with ICP-MS provides a new strategy for the analysis of trace metals in cells.

Advances in ICP-MS-based techniques for trace elements and their species analysis in cells

2017 ◽  
Vol 32 (9) ◽  
pp. 1650-1659 ◽  
Author(s):  
Han Wang ◽  
Man He ◽  
Beibei Chen ◽  
Bin Hu
Keyword(s):  
Trace Elements ◽  
State Of The Art ◽  
The State ◽  
Icp Ms ◽  
Species Analysis ◽  
In Cells

The state-of-the-art ICP-MS based methods for trace elements and their species analysis in cells are reviewed.

Speciation of selenium in cells by HPLC-ICP-MS after (on-chip) magnetic solid phase extraction

2013 ◽  
Vol 28 (3) ◽  
pp. 334 ◽  
Author(s):  
Beibei Chen ◽  
Bin Hu ◽  
Man He ◽  
Qian Huang ◽  
Yuan Zhang ◽  
...  
Keyword(s):  
Solid Phase Extraction ◽  
Solid Phase ◽  
Magnetic Solid Phase Extraction ◽  
Phase Extraction ◽  
Icp Ms ◽  
Magnetic Solid ◽  
On Chip ◽  
In Cells

scholarly journals Oligo(dA-dT)-dependent signal amplification for the detection of proteins in cells

BioTechniques ◽  
2004 ◽  
Vol 36 (5) ◽  
pp. 856-863 ◽  
Author(s):  
Ken-ichi Hanaki ◽  
Seii Ohka ◽  
Kenji Yamamoto ◽  
Akio Nomoto ◽  
Hiroshi Yoshikura
Keyword(s):  
Signal Amplification ◽  
Dependent Signal ◽  
In Cells
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