Antigenic characterization of influenza and SARS-CoV-2 viruses

To evaluate the antigenic properties of Hepatitis E Virus (HEV) Open Reading Frame 2 and 3 (ORF2 and ORF3) codified proteins, we expressed different portions of ORF2 and the entire ORF3 in E. coli, a truncated ORF2, was also expressed in baculovirus. A panel of 37 monoclonal antibodies (MAbs) was raised against ORF2 (1–660 amino acids) and MAbs were mapped and characterized using the ORF2 expressed portions. Selected HEV positive and negative swine sera were used to evaluate ORF2 and ORF3 antigens’ immunogenicity. The MAbs were clustered in six groups identifying six antigenic regions along the ORF2. Only MAbs binding to the sixth ORF2 antigenic region (394–608 aa) were found to compete with HEV positive sera and efficiently catch the recombinant antigen expressed in baculovirus. The ORF2 portion from 394–608 aa demonstrated to include most immunogenic epitopes with 85% of HEV positive swine sera reacting against the region from 461–544 aa. Only 5% of the selected HEV sera reacted against the ORF3 antigen.

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Antigenic characterization of novel H1 influenza A viruses in swine

Scientific Reports ◽

10.1038/s41598-020-61315-5 ◽

2020 ◽

Vol 10 (1) ◽

Cited By ~ 2

Author(s):

Rodrigo Tapia ◽

Montserrat Torremorell ◽

Marie Culhane ◽

Rafael A. Medina ◽

Víctor Neira

Keyword(s):

Influenza A ◽

Influenza A Viruses ◽

Antigenic Characterization

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Antigenic Characterization of an Arkansas Isolate of Infectious Bursal Disease Virus

Avian Diseases ◽

10.2307/1592269 ◽

1996 ◽

Vol 40 (3) ◽

pp. 588 ◽

Cited By ~ 2

Author(s):

G. R. Bayyari ◽

J. D. Story ◽

J. N. Beasley ◽

J. K. Skeeles

Keyword(s):

Disease Virus ◽

Infectious Bursal Disease Virus ◽

Infectious Bursal Disease ◽

Antigenic Characterization ◽

Bursal Disease

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Cloning, expression, and antigenic characterization of recombinant protein ofMycoplasma gallisepticum expressed inEscherichia coli

Poultry Science ◽

10.3382/ps/pev012 ◽

2015 ◽

Vol 94 (4) ◽

pp. 621-627 ◽

Cited By ~ 1

Author(s):

T.S. Rocha ◽

C. Tramuta ◽

S. Catania ◽

A. Matucci ◽

M.G. Giuffrida ◽

...

Keyword(s):

Recombinant Protein ◽

Inescherichia Coli ◽

Antigenic Characterization

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Genetic and Antigenic Characterization of Avian Avulavirus Type 6 (AAvV-6) Circulating in Canadian Wild Birds (2005–2017)

Viruses ◽

10.3390/v13040543 ◽

2021 ◽

Vol 13 (4) ◽

pp. 543

Author(s):

Tamiko Hisanaga ◽

Catherine Soos ◽

Nicola Lewis ◽

Oliver Lung ◽

Matthew Suderman ◽

...

Keyword(s):

Clinical Symptoms ◽

Fusion Gene ◽

Full Genome ◽

Genome Sequences ◽

Viral Shedding ◽

Genetic Groups ◽

Antigenic Characterization ◽

Specific Pathogen ◽

First Time

We describe for the first time the genetic and antigenic characterization of 18 avian avulavirus type-6 viruses (AAvV-6) that were isolated from wild waterfowl in the Americas over the span of 12 years. Only one of the AAvV-6 viruses isolated failed to hemagglutinate chicken red blood cells. We were able to obtain full genome sequences of 16 and 2 fusion gene sequences from the remaining 2 isolates. This is more than double the number of full genome sequences available at the NCBI database. These AAvV-6 viruses phylogenetically grouped into the 2 existing AAvV-6 genotype subgroups indicating the existence of an intercontinental epidemiological link with other AAvV-6 viruses isolated from migratory waterfowl from different Eurasian countries. Antigenic maps made using HI assay data for these isolates showed that the two genetic groups were also antigenically distinct. An isolate representing each genotype was inoculated in specific pathogen free (SPF) chickens, however, no clinical symptoms were observed. A duplex fusion gene based real-time assay for the detection and genotyping of AAvV-6 to genotype 1 and 2 was developed. Using the developed assay, the viral shedding pattern in the infected chickens was examined. The chickens infected with both genotypes were able to shed the virus orally for about a week, however, no significant cloacal shedding was detected in chickens of both groups. Chickens in both groups developed detectable levels of anti-hemagglutinin antibodies 7 days after infection.

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Molecular identification and antigenic characterization of Babesia divergens Erythrocyte Binding Protein (BdEBP) as a potential vaccine candidate

Parasitology International ◽

10.1016/j.parint.2017.07.004 ◽

2017 ◽

Vol 66 (6) ◽

pp. 721-726 ◽

Cited By ~ 3

Author(s):

Shimaa Abd El-Salam El-Sayed ◽

Mohamed Abdo Rizk ◽

Mohamad Alaa Terkawi ◽

Naoaki Yokoyama ◽

Ikuo Igarashi

Keyword(s):

Molecular Identification ◽

Vaccine Candidate ◽

Binding Protein ◽

Potential Vaccine Candidate ◽

Antigenic Characterization ◽

Babesia Divergens ◽

Erythrocyte Binding ◽

Potential Vaccine

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Antigenic characterization of a formalin-inactivated poliovirus vaccine derived from live-attenuated Sabin strains

Vaccine ◽

10.1016/j.vaccine.2007.07.060 ◽

2007 ◽

Vol 25 (41) ◽

pp. 7041-7046 ◽

Cited By ~ 35

Author(s):

Yoshio Tano ◽

Hiroyuki Shimizu ◽

Javier Martin ◽

Yorihiro Nishimura ◽

Bunsiti Simizu ◽

...

Keyword(s):

Antigenic Characterization

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Identification and Characterization of P0 Protein as a Vaccine Candidate Against Babesia divergens, Blood Parasite of Veterinary and Zoonotic Importance

Frontiers in Veterinary Science ◽

10.3389/fvets.2021.795906 ◽

2022 ◽

Vol 8 ◽

Author(s):

Shimaa Abd El-Salam El-Sayed ◽

Mohamed Abdo Rizk ◽

Haitham Eldoumani ◽

Shimaa Sobhy Sorour ◽

Mohamad Alaa Terkawi ◽

...

Keyword(s):

Vaccine Candidate ◽

Fluorescent Antibody ◽

Specific Interaction ◽

Blood Parasite ◽

Antigenic Characterization ◽

Babesia Divergens ◽

Field Samples ◽

P0 Protein

The molecular identification and antigenic characterization of P0 protein in Babesia divergens, a blood parasite of veterinary and zoonotic importance, were carried out in this study for use in developing subunit vaccines against B. divergens infection. Recombinant protein encoding P0 (BdP0) was developed in Escherichia coli, and its antiserum was generated in mice for further molecular characterization. Anti-rBdP0 serum had a specific interaction with the corresponding legitimate B. divergens protein, as confirmed by Western blotting and indirect fluorescent antibody tests. ELISA was used to assess the immunogenicity of BdP0 in a group of 68 bovine field samples, and significant immunological reactivity was found in 19 and 20 positive samples of rBdp0 and B. divergens lysate, respectively. The in vitro growth of B. divergens cultures treated with anti-rBdP0 serum was significantly inhibited (p < 0.05). Furthermore, after 6 h of incubation with 2 mg/ml anti-rBdP0 serum, the ability of pre-incubated free merozoites to invade bovine erythrocytes was reduced by 59.88%. The obtained data suggest the possible use of rBdP0 as diagnostic antigen and may serve as a vaccine candidate against babesiosis caused by B. divergens either in animal or human.

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Glycan remodeled erythrocytes facilitate antigenic characterization of recent A/H3N2 influenza viruses

10.1101/2020.12.18.423398 ◽

2020 ◽

Author(s):

Frederik Broszeit ◽

Rosanne J. van Beek ◽

Luca Unione ◽

Theo M. Bestebroer ◽

Digantkumar Chapla ◽

...

Keyword(s):

Influenza Viruses ◽

Side Chain ◽

Computational Studies ◽

Receptor Binding Site ◽

Adaptive Mutations ◽

Antibody Recognition ◽

Antigenic Characterization ◽

H3n2 Influenza ◽

Human Receptor

AbstractDuring circulation in humans and natural selection to escape antibody recognition for decades, A/H3N2 influenza viruses emerged with altered receptor specificities. These viruses lost the ability to agglutinate erythrocytes critical for antigenic characterization and give low yields and acquire adaptive mutations when cultured in eggs and cells, contributing to recent vaccine challenges. We examined receptor specificities of A/H3N2 viruses, revealing that recent viruses compensated for decreased binding of the prototypic human receptor by recognizing 2,6-sialosides on extended LacNAc moieties. Erythrocyte glycomics showed an absence of extended glycans, providing a rationale for lack of agglutination by recent A/H3N2 viruses. A glycan remodeling approach installed functional receptors on erythrocytes, allowing antigenic characterization of recent A/H3N2 viruses and confirming the cocirculation of several antigenically different viruses in humans. Computational studies of HAs in complex with a sialoside having an extended LacNAc moiety revealed that mutations distal to the RBD reoriented the Y159 side chain, resulting in an extended receptor binding site.

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