Large-scale production of biologically active human keratinocyte growth factor-2

2009 ◽  
Vol 82 (3) ◽  
pp. 439-444 ◽  
Author(s):  
Xiaoping Wu ◽  
Haishan Tian ◽  
Yadong Huang ◽  
Sixian Wu ◽  
Xiaoju Liu ◽  
...  
Keyword(s):  
Growth Factor ◽  
Large Scale ◽  
Keratinocyte Growth Factor ◽  
Biologically Active ◽  
Scale Production ◽  
Human Keratinocyte ◽  
Large Scale Production

scholarly journals Plant expression systems for production of hemagglutinin as a vaccine against influenza virus.

2014 ◽  
Vol 61 (3) ◽  
Author(s):  
Patrycja Redkiewicz ◽  
Agnieszka Sirko ◽  
Katarzyna Anna Kamel ◽  
Anna Góra-Sochacka
Keyword(s):  
Influenza Virus ◽  
Large Scale ◽  
Immunological Response ◽  
Biologically Active ◽  
Scale Production ◽  
Expression Systems ◽  
Lethal Challenge ◽  
Subunit Vaccines ◽  
Large Scale Production ◽  
Major Surface

Many examples of a successful application of plant-based expression systems for production of biologically active recombinant proteins exist in the literature. These systems can function as inexpensive platforms for the large scale production of recombinant pharmaceuticals or subunit vaccines. Hemagglutinin (HA) is a major surface antigen of the influenza virus, thus it is in the centre of interests of various subunit vaccine engineering programs. Large scale production of recombinant HA in traditional expression systems, such as mammalian or insect cells, besides other limitations, is expensive and time-consuming. These difficulties stimulate an ever-increasing interest in plant-based production of this recombinant protein. Over the last few years many successful cases of HA production in plants, using both transient and stable expression systems have been reported. Various forms of recombinant HA, including monomers, trimers, virus like particles (VLPs) or chimeric proteins containing its fusion with other polypeptides were obtained and shown to maintain a proper antigenicity. Immunizations of animals (mice, ferrets, rabbits or chickens) with some of these plant-derived hemagglutinin variants were performed, and their effectiveness in induction of immunological response and protection against lethal challenge with influenza virus demonstrated. Plant-produced recombinant subunit vaccines and plant-made VLPs were successfully tested in clinical trials (Phase I and II) that confirmed their tolerance and immunogenicity.

Dimroth´s Rearrangement as a Synthetic Strategy Towards New Heterocyclic Compounds

2020 ◽  
Vol 24 (17) ◽  
pp. 1999-2018
Author(s):  
Vitor F. Ferreira ◽  
Thais de B. da Silva ◽  
Fernanda P. Pauli ◽  
Patricia G. Ferreira ◽  
Luana da S. M. Forezi ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Heterocyclic Compounds ◽  
Large Scale ◽  
Molecular Diversity ◽  
Biologically Active ◽  
Scale Production ◽  
Dimroth Rearrangement ◽  
Synthetic Strategy ◽  
Large Scale Production ◽  
Synthetic Approaches

Molecular rearrangements are important tools to increase the molecular diversity of new bioactive compounds, especially in the class of heterocycles. This review deals specifically with a very famous and widely applicable rearrangement known as the Dimroth Rearrangement. Although it has originally been observed for 1,2,3-triazoles, its amplitude was greatly expanded to other heterocycles, as well as from laboratory to large scale production of drugs and intermediates. The reactions that were discussed in this review were selected with the aim of demonstrating the windows that may be open by the Dimroth's rearrangement, especially in what regards the development of new synthetic approaches toward biologically active compounds.

Cloning, large-scale production, and purification of active dimeric rat vascular endothelial growth factor (rrVEGF-164)

2010 ◽  
Vol 69 (1) ◽  
pp. 76-82 ◽  
Author(s):  
Paul J. Geutjes ◽  
Suzan T.M. Nillesen ◽  
Gerwen Lammers ◽  
Willeke F. Daamen ◽  
Toin H. van Kuppevelt
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Large Scale ◽  
Scale Production ◽  
Vascular Endothelial ◽  
Large Scale Production ◽  
Endothelial Growth Factor

Development of dairy herd of transgenic goats as biofactory for large-scale production of biologically active recombinant human lactoferrin

2019 ◽  
Vol 28 (5-6) ◽  
pp. 465-478 ◽  
Author(s):  
I. Semak ◽  
A. Budzevich ◽  
E. Maliushkova ◽  
V. Kuzniatsova ◽  
N. Popkov ◽  
...  
Keyword(s):  
Large Scale ◽  
Dairy Herd ◽  
Biologically Active ◽  
Human Lactoferrin ◽  
Scale Production ◽  
Recombinant Human Lactoferrin ◽  
Large Scale Production ◽  
Transgenic Goats

Secretion of the Antibacterial Recombinant Protein Enbocin

2008 ◽  
Vol 63 (3-4) ◽  
pp. 284-288 ◽  
Author(s):  
Tae Won Goo ◽  
Eun Young Yun ◽  
Sung Wan Kim ◽  
Kwang Ho Choi ◽  
Seok Woo Kang ◽  
...  
Keyword(s):  
Recombinant Proteins ◽  
Gene Fusion ◽  
Large Scale ◽  
Biologically Active ◽  
Vector System ◽  
Fusion Partner ◽  
Scale Production ◽  
Baculovirus Expression ◽  
Large Scale Production ◽  
Baculovirus Expression Vector

The insect baculovirus expression vector system (BEVS) is useful for the production of biologically active recombinant proteins. However, the overexpression of foreign proteins in this system often results in misfolded proteins and the formation of protein aggregates. To overcome this limitation, we have developed a versatile baculovirus expression and secretion system using the Bombyx mori protein disulfide isomerase (bPDI) as a fusion partner. bPDI gene fusion improved the secretion and antibacterial activity of recombinant enbocin proteins. Thus, bPDI gene fusion is a useful addition to the BEVS for the large-scale production of bioactive recombinant proteins

Applications, and Efficient Large-Scale Production, of Recombinant Human Epidermal Growth Factor

2001 ◽  
Vol 18 (1) ◽  
pp. 51-71 ◽  
Author(s):  
Wan-Keung R. Wong ◽  
Edward Lam R.C. Huang ◽  
Raymond S.C. Wong ◽  
Christina Morris ◽  
Jim Hackett
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Large Scale ◽  
Scale Production ◽  
Human Epidermal Growth Factor ◽  
Large Scale Production ◽  
Epidermal Growth
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