Lipidome profiling of Saccharomyces cerevisiae reveals pitching rate-dependent fermentative performance

2010 ◽  
Vol 87 (4) ◽  
pp. 1507-1516 ◽  
Author(s):  
Hong-Chi Tian ◽  
Jian Zhou ◽  
Bin Qiao ◽  
Ying Liu ◽  
Jin-Mei Xia ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Rate Dependent ◽  
Pitching Rate

scholarly journals Transcription factor control of growth rate dependent genes in Saccharomyces cerevisiae: A three factor design

BMC Genomics ◽  
2008 ◽  
Vol 9 (1) ◽  
pp. 341 ◽  
Author(s):  
Alessandro Fazio ◽  
Michael C Jewett ◽  
Pascale Daran-Lapujade ◽  
Roberta Mustacchi ◽  
Renata Usaite ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Growth Rate ◽  
Transcription Factor ◽  
Rate Dependent ◽  
Factor Design ◽  
Three Factor

scholarly journals Physiological and Transcriptional Responses of Saccharomyces cerevisiae to Zinc Limitation in Chemostat Cultures

2007 ◽  
Vol 73 (23) ◽  
pp. 7680-7692 ◽  
Author(s):  
Raffaele De Nicola ◽  
Lucie A. Hazelwood ◽  
Erik A. F. De Hulster ◽  
Michael C. Walsh ◽  
Theo A. Knijnenburg ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Growth Rate ◽  
Transcriptional Response ◽  
Context Dependency ◽  
Transcriptional Responses ◽  
Combinatorial Regulation ◽  
Rate Dependent ◽  
Responsive Element ◽  
And Function ◽  
Zn Availability

ABSTRACT Transcriptional responses of the yeast Saccharomyces cerevisiae to Zn availability were investigated at a fixed specific growth rate under limiting and abundant Zn concentrations in chemostat culture. To investigate the context dependency of this transcriptional response and eliminate growth rate-dependent variations in transcription, yeast was grown under several chemostat regimens, resulting in various carbon (glucose), nitrogen (ammonium), zinc, and oxygen supplies. A robust set of genes that responded consistently to Zn limitation was identified, and the set enabled the definition of the Zn-specific Zap1p regulon, comprised of 26 genes and characterized by a broader zinc-responsive element consensus (MHHAACCBYNMRGGT) than so far described. Most surprising was the Zn-dependent regulation of genes involved in storage carbohydrate metabolism. Their concerted down-regulation was physiologically relevant as revealed by a substantial decrease in glycogen and trehalose cellular content under Zn limitation. An unexpectedly large number of genes were synergistically or antagonistically regulated by oxygen and Zn availability. This combinatorial regulation suggested a more prominent involvement of Zn in mitochondrial biogenesis and function than hitherto identified.

Evaluation of Freezing Methods by Low Temperature X-Ray Diffraction

1977 ◽  
Vol 35 ◽  
pp. 330-333
Author(s):  
T. Gulik-Krzywicki ◽  
M.J. Costello
Keyword(s):  
Biological Materials ◽  
Molecular Organization ◽  
X Ray Diffraction ◽  
Glass Capillary ◽  
X Ray ◽  
Rate Dependent ◽  
Before And After ◽  
Freeze Etching ◽  
Diffraction Patterns ◽  
Set Up

Freeze-etching electron microscopy is currently one of the best methods for studying molecular organization of biological materials. Its application, however, is still limited by our imprecise knowledge about the perturbations of the original organization which may occur during quenching and fracturing of the samples and during the replication of fractured surfaces. Although it is well known that the preservation of the molecular organization of biological materials is critically dependent on the rate of freezing of the samples, little information is presently available concerning the nature and the extent of freezing-rate dependent perturbations of the original organizations. In order to obtain this information, we have developed a method based on the comparison of x-ray diffraction patterns of samples before and after freezing, prior to fracturing and replication.Our experimental set-up is shown in Fig. 1. The sample to be quenched is placed on its holder which is then mounted on a small metal holder (O) fixed on a glass capillary (p), whose position is controlled by a micromanipulator.

Anti-Saccharomyces cerevisiae Antibodies in Inflammatory Bowel Disease: a Family Study

2001 ◽  
Vol 36 (2) ◽  
pp. 196-201 ◽  
Author(s):  
F. Seibold ◽  
O. Stich ◽  
R. Hufnagl ◽  
S. Kamil ◽  
M. Scheurlen
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Inflammatory Bowel Disease ◽  
Bowel Disease ◽  
Family Study ◽  
Inflammatory Bowel

Rate-Dependent Speech Processing Can be Domain Specific

2012 ◽  
Author(s):  
Christine M. Szostak ◽  
Mark A. Pitt ◽  
Laura C. Dilley
Keyword(s):  
Speech Processing ◽  
Domain Specific ◽  
Rate Dependent
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