Response surface methodology to design a selective enrichment broth for rapid detection of Salmonella spp. by SYBR Green Ι real-time PCR

2013 ◽  
Vol 97 (9) ◽  
pp. 4149-4158 ◽  
Author(s):  
Qiaoyan Zhang ◽  
Tingting Chen ◽  
Shengli Yang ◽  
Xiaofu Wang ◽  
Hui Guo
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Sybr Green ◽  
Salmonella Spp ◽  
Enrichment Broth ◽  
Selective Enrichment ◽  
Selective Enrichment Broth

Rapid Detection for Salmonella spp. by Ultrafast Real-time PCR Assay

2018 ◽  
Vol 33 (1) ◽  
pp. 50-57
Author(s):  
Seok Hwan Kim ◽  
Yu-Si Lee ◽  
In-Sun Joo ◽  
Hyo Sun Kwak ◽  
Gyung Tae Chung ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Pcr Assay ◽  
Salmonella Spp

RAPID DETECTION OF MYCOPLASMA CONTAMINATION IN CELL CULTURES USING SYBR GREEN-BASED REAL-TIME PCR

2006 ◽  
Author(s):  
Yoko Ishikawa ◽  
Takaharu Kozakai ◽  
Hatsue Morita ◽  
Kaname Saida ◽  
Syuichi Oka ◽  
...  
Keyword(s):  
Real Time ◽  
Cell Cultures ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Sybr Green ◽  
Mycoplasma Contamination

SYBR Green I-based real-time PCR targeting the rpoX gene for sensitive and rapid detection of Vibrio alginolyticus

2011 ◽  
Vol 25 (2-3) ◽  
pp. 137-141 ◽  
Author(s):  
Zhao Jing-jing ◽  
Chen Chang ◽  
Luo Peng ◽  
Ren Chun-hua ◽  
Jiang Xiao ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Vibrio Alginolyticus ◽  
Sybr Green ◽  
Sybr Green I ◽  
Pcr Targeting

A SYBR Green based multiplex Real-Time PCR assay for rapid detection and differentiation of ocular bacterial pathogens

2020 ◽  
Vol 171 ◽  
pp. 105875 ◽  
Author(s):  
Deepthi KrishnanNair Geetha ◽  
Balaji Sivaraman ◽  
Ram Rammohan ◽  
Narendran Venkatapathy ◽  
Prabagaran Solai Ramatchandirane
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Bacterial Pathogens ◽  
Sybr Green ◽  
Pcr Assay

scholarly journals Evaluation of a Rapid Culture-Based Screening Test for Detection of Methicillin Resistant Staphylococcus aureus

2011 ◽  
Vol 60 (3) ◽  
pp. 265-268
Author(s):  
BOUSHRA FWITY ◽  
RALF LOBMANN ◽  
ANDREAS AMBROSCH
Keyword(s):  
Staphylococcus Aureus ◽  
Rapid Detection ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Detection Method ◽  
Latex Agglutination ◽  
Enrichment Broth ◽  
Methicillin Resistant ◽  
Selective Enrichment ◽  
Vitek 2 ◽  
Selective Enrichment Broth

The performance of a culture based assay, BacLite Rapid MRSA for the rapid detection (5 hours) of methicillin resistant Staphylococcus aureus (MRSA) from specimens (n = 377) obtained from nares, throat, wounds and perineum was investigated. Compared to culture based reference methods (chromogenic MRSA ID (bioMerieux)), selective enrichment broth, PBP2' latex agglutination (Oxoid) and VITEK 2 identification (bioMerieux), an overall sensitivity of 71% with a 82% specificity and a negative predictive value (NPV) of 95% was provided. The Baclite test is rapid and easy to use and has the advantage of a culture-based detection method for MRSA.

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