scholarly journals Evaluation of a Rapid Culture-Based Screening Test for Detection of Methicillin Resistant Staphylococcus aureus

2011 ◽  
Vol 60 (3) ◽  
pp. 265-268
Author(s):  
BOUSHRA FWITY ◽  
RALF LOBMANN ◽  
ANDREAS AMBROSCH
Keyword(s):  
Staphylococcus Aureus ◽  
Rapid Detection ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Detection Method ◽  
Latex Agglutination ◽  
Enrichment Broth ◽  
Methicillin Resistant ◽  
Selective Enrichment ◽  
Vitek 2 ◽  
Selective Enrichment Broth

The performance of a culture based assay, BacLite Rapid MRSA for the rapid detection (5 hours) of methicillin resistant Staphylococcus aureus (MRSA) from specimens (n = 377) obtained from nares, throat, wounds and perineum was investigated. Compared to culture based reference methods (chromogenic MRSA ID (bioMerieux)), selective enrichment broth, PBP2' latex agglutination (Oxoid) and VITEK 2 identification (bioMerieux), an overall sensitivity of 71% with a 82% specificity and a negative predictive value (NPV) of 95% was provided. The Baclite test is rapid and easy to use and has the advantage of a culture-based detection method for MRSA.

A pragmatic eLCR for an ultrasensitive detection of methicillin-resistant Staphylococcus aureus in joint synovial fluid: superior to qPCR

The Analyst ◽  
2021 ◽  
Author(s):  
Jinyuan Chen ◽  
Hongxiang Wei ◽  
Xinyu Fang ◽  
Yuanqing Cai ◽  
Zhenzhen Zhang ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Synovial Fluid ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Detection Method ◽  
Low Cost ◽  
Ultrasensitive Detection ◽  
Gene Detection ◽  
Methicillin Resistant ◽  
Cost Identification

A pragmatic electrochemical mecA gene detection method for a rapid, accurate and low-cost identification of MRSA in the joint synovial fluid of PJI patients.

scholarly journals Detection of mecA gene by latex agglutination and its molecular analysis by PCR in methicillin resistant staphylococcus aureus.

2020 ◽  
Vol 27 (07) ◽  
pp. 1363-1370
Author(s):  
Aneela Khawaja ◽  
Iffat Javed ◽  
Sohaila Mushtaq ◽  
Saeed Anwar ◽  
Faiqa Arshad ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
Meca Gene ◽  
Latex Agglutination ◽  
Medical Institute ◽  
Cross Sectional ◽  
Methicillin Resistant ◽  
Agglutination Method ◽  
Resistant Strains

Antimicrobial resistance (AMR) is a devastating question that is threatening the health globally. The extensive and indiscriminative use of antibiotics has evolved a notorious resistance in Staphylococcus aureus.  This resistance developed through possession of mecA gene, which codes for modified penicillin binding protein (PBP2a) and the emergent strain being labeled “methicillin resistant Staphylococcus aureus”. Conventional phenotypic techniques for detection of MRSA rely on standardization of cultural characteristics. The drawbacks of diagnostic error to report MRSA include: poor prognosis, expensive treatment, dissemination of multi-drug resistant strains and even treatment failure. Latex agglutination method can be adopted as a more accurate and quick strategy for rapid detection of methicillin resistance. Objectives: To compare detection of mecA gene in methicillin resistant isolates of Staphylococcus aureus by latex agglutination and PCR; by assessing the sensitivity and specificity of both methods. Study Design: Descriptive Cross-Sectional study. Setting: Pathology Department, Post Graduate Medical Institute, Lahore. Period: From January 2015 to December 2015; according to standard operating procedures at Microbiology laboratory. Material & Methods: A total 713 consecutive, non-duplicate isolates of Staphylococcus aureus were processed. Methicillin resistance was determined using cefoxitin (30mg) by Kirby-Bauer method using CLSI guideline (2016), latex agglutination method; and PCR for mecA gene. Results: The results showed that out of 713 Staphylococcus aureus isolates, 92 (12.90%) isolates were resistant to cefoxitin and were labelled as MRSA. majority MRSA isolates recovered from pus (44.57%) and wound swab (20.65%), followed by blood (13.04%), fluid (8.70%), CSF (4.35%), CVP (3.26%), HVS (3.26%) and tracheal secretion (2.17%). By latex agglutination method, 87 (94.50%) were positive for PBP2a; while on PCR mecA gene was detected only in 82 (89.10%) MRSA isolates. When assessed with PCR (gold standard) the sensitivity and diagnostic accuracy of latex agglutination was 100% and 94.57%, respectively. Conclusion: Latex agglutination test can be employed as rapid and reliable diagnostic technique in MRSA isolates for mecA gene detection, where resources for molecular methods are inadequate. This can effectually lessen the misdiagnosis of resistant strains, and over/ ill-use of antibiotics.

scholarly journals Prevalence of Staphylococcus aureus and Methicillin-resistant S. aureus in Adult Hospitalized Patients in “Mother Theresa” Hospital Center in Albania

2020 ◽  
Vol 8 (A) ◽  
pp. 297-302
Author(s):  
Blerta Kika ◽  
Erjona Abazaj ◽  
Oltiana Petri ◽  
Andi Koraqi
Keyword(s):  
Staphylococcus Aureus ◽  
Rapid Detection ◽  
Latex Agglutination ◽  
Hospitalized Patients ◽  
Hospital Environment ◽  
Drug Resistant ◽  
Methicillin Resistant ◽  
Clinical Specimens ◽  
Microbial Identification ◽  
Hospital Center

AIM: The aim of this study was to evaluate the prevalence of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) in clinical specimens hospitalized to “Mother Theresa” Hospital Center for 2 years. METHODS: We isolated and identified S. aureus on 356 clinical specimens using standard tests. Furthermore, for further accurate microbial identification, we have to use the VITEK® 2 system. The samples were tested to detect the presence of MRSA by a slide latex agglutination kit for the rapid detection of PBP2. RESULTS: The overall prevalence of S. aureus in patients was 34.2%. The prevalence of MRSA was 20.5% of cases. Of the MRSA isolates identified in this study, 28% were susceptible to antibiotics, 24% demonstrated intermediate resistance, and 48% were multi-drug resistant with resistance to nineteen antibiotics involved in the examination. In addition, seven of the 25 MRSA cases showed 100% resistance to norfloxacin, imipenem, meropenem, levofloxacin, etc. CONCLUSIONS: The rate of S. aureus in hospitalized patients on this study was 34.2% and the MRSA 20.5%. These results indicated that this type of infection is a significant concern for health services and patients included. A screening of all hospitalized cases can lead to reduce the incidence of this infection in the hospital environment.

COMPARATIVE STUDY OF E-STRIP AND VITEK 2 FOR VANCOMYCIN MIC FOR STAPHYLOCOCCUS AUREUS AT TERTIARY CARE CENTER

2021 ◽  
pp. 8-10
Author(s):  
Gauri Thakare ◽  
Sonal Chavan ◽  
Sharmila Raut ◽  
Rajani Tore ◽  
Ravindra Khadse
Keyword(s):  
Staphylococcus Aureus ◽  
Comparative Study ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Care Center ◽  
Tertiary Care ◽  
Test Material ◽  
Methicillin Resistant ◽  
Study Results ◽  
Vitek 2 ◽  
Strip Test

PURPOSE: Comparative Study done for Vancomycin susceptibility in Methicillin Resistant Staphylococcus aureus (MRSA) in two methods Vitek-2 and E-strip test. MATERIAL AND METHOD: Vancomycin susceptibility testing was performed on these Methicillin Resistant Staphylococcus aureus (MRSA) isolates by two methods viz. Vitek 2 & E- strip Test. RESULT: A total of 10680 various specimens were received and processed in laboratory. 210 samples were S. aureus amongst which 76 were MRSAs. Pus was the predominant sample followed by endotracheal secretions, blood, and sputum. All the strains of MRSA found susceptible to vancomycin (MIC≤ 2µg/ ml) by both the methods. Maximum specimens by both the methods had MICs of 1.0 µg/ ml. CONCLUSION: In the present study results of Vitek and E-strip were almost comparable. In the advanced era of automation and computerized technology with manpower compromised labs, Vitek could be a better option for vancomycin MIC.

scholarly journals Thin Film Biosensor for Rapid Detection of mecA from Methicillin-resistant Staphylococcus aureus

2000 ◽  
Vol 46 (9) ◽  
pp. 1501-1504 ◽  
Author(s):  
Robert Jenison ◽  
Ayla Haeberli ◽  
Shao Yang ◽  
Barry Polisky ◽  
Rachel Ostroff
Keyword(s):  
Thin Film ◽  
Staphylococcus Aureus ◽  
Rapid Detection ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Methicillin Resistant
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