scholarly journals Diversity, enrichment, and genomic potential of anaerobic methane- and ammonium-oxidizing microorganisms from a brewery wastewater treatment plant

2020 ◽  
Vol 104 (16) ◽  
pp. 7201-7212 ◽  
Author(s):  
Karin Stultiens ◽  
Maartje A.H.J. van Kessel ◽  
Jeroen Frank ◽  
Peter Fischer ◽  
Chris Pelzer ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plant ◽  
Oxidation Rate ◽  
Enrichment Culture ◽  
Treatment Plant ◽  
Marker Genes ◽  
Anammox Bacteria ◽  
Ammonium Oxidation ◽  
Brewery Wastewater ◽  
Activity Measurements

Abstract Anaerobic wastewater treatment offers several advantages; however, the effluent of anaerobic digesters still contains high levels of ammonium and dissolved methane that need to be removed before these effluents can be discharged to surface waters. The simultaneous anaerobic removal of methane and ammonium by denitrifying (N-damo) methanotrophs in combination with anaerobic ammonium-oxidizing (anammox) bacteria could be a potential solution to this challenge. After a molecular survey of a wastewater plant treating brewery effluent, indicating the presence of both N-damo and anammox bacteria, we started an anaerobic bioreactor with a continuous supply of methane, ammonium, and nitrite to enrich these anaerobic microorganisms. After 14 months of operation, a stable enrichment culture containing two types of ‘Candidatus Methylomirabilis oxyfera’ bacteria and two strains of ‘Ca. Brocadia’-like anammox bacteria was achieved. In this community, anammox bacteria converted 80% of the nitrite with ammonium, while ‘Ca. Methylomirabilis’ contributed to 20% of the nitrite consumption. The analysis of metagenomic 16S rRNA reads and fluorescence in situ hybridization (FISH) correlated well and showed that, after 14 months, ‘Ca. Methylomirabilis’ and anammox bacteria constituted approximately 30 and 20% of the total microbial community. In addition, a substantial part (10%) of the community consisted of Phycisphaera-related planctomycetes. Assembly and binning of the metagenomic sequences resulted in high-quality draft genome of two ‘Ca. Methylomirabilis’ species containing the marker genes pmoCAB, xoxF, and nirS and putative NO dismutase genes. The anammox draft genomes most closely related to ‘Ca. Brocadia fulgida’ included the marker genes hzsABC, hao, and hdh. Whole-reactor and batch anaerobic activity measurements with methane, ammonium, nitrite, and nitrate revealed an average anaerobic methane oxidation rate of 0.12 mmol h−1 L−1 and ammonium oxidation rate of 0.5 mmol h−1 L−1. Together, this study describes the enrichment and draft genomes of anaerobic methanotrophs from a brewery wastewater treatment plant, where these organisms together with anammox bacteria can contribute significantly to the removal of methane and ammonium in a more sustainable way. Key points • An enrichment culture containing both N-damo and anammox bacteria was obtained. • Simultaneous consumption of ammonia, nitrite, and methane under anoxic conditions. • In-depth metagenomic biodiversity analysis of inoculum and enrichment culture.

scholarly journals Simultaneous Nitrite-Dependent Anaerobic Methane and Ammonium Oxidation Processes

2011 ◽  
Vol 77 (19) ◽  
pp. 6802-6807 ◽  
Author(s):  
Francisca A. Luesken ◽  
Jaime Sánchez ◽  
Theo A. van Alen ◽  
Janeth Sanabria ◽  
Huub J. M. Op den Camp ◽  
...  
Keyword(s):  
Enrichment Culture ◽  
Anammox Bacteria ◽  
Anaerobic Oxidation Of Methane ◽  
Ammonium Oxidation ◽  
Content Type ◽  
Oxidation Of Methane ◽  
Activity Measurements ◽  
Near Future ◽  
Damo Bacteria

ABSTRACTNitrite-dependent anaerobic oxidation of methane (n-damo) and ammonium (anammox) are two recently discovered processes in the nitrogen cycle that are catalyzed by n-damo bacteria, including “CandidatusMethylomirabilis oxyfera,” and anammox bacteria, respectively. The feasibility of coculturing anammox and n-damo bacteria is important for implementation in wastewater treatment systems that contain substantial amounts of both methane and ammonium. Here we tested this possible coexistence experimentally. To obtain such a coculture, ammonium was fed to a stable enrichment culture of n-damo bacteria that still contained some residual anammox bacteria. The ammonium supplied to the reactor was consumed rapidly and could be gradually increased from 1 to 20 mM/day. The enriched coculture was monitored by fluorescencein situhybridization and 16S rRNA andpmoAgene clone libraries and activity measurements. After 161 days, a coculture with about equal amounts of n-damo and anammox bacteria was established that converted nitrite at a rate of 0.1 kg-N/m3/day (17.2 mmol day−1). This indicated that the application of such a coculture for nitrogen removal may be feasible in the near future.

scholarly journals Metagenome Sequences of a Wastewater Treatment Plant Digester Sludge-Derived Enrichment Culture

2020 ◽  
Vol 9 (32) ◽  
Author(s):  
Heiko Nacke ◽  
Laura L. Kirck ◽  
Sophia Schwarz ◽  
Dominik Schneider ◽  
Anja Poehlein ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Microbial Community ◽  
Wastewater Treatment Plant ◽  
Enrichment Culture ◽  
Treatment Plant ◽  
Methanogenic Archaea ◽  
Metagenomic Analysis ◽  
Strictly Anaerobic ◽  
Anaerobic Conditions ◽  
Genes Encoding

ABSTRACT We sequenced the metagenome of a microbial community enriched under strictly anaerobic conditions from wastewater treatment plant-derived digester sludge. The metagenomic analysis of the enrichment revealed that Acetobacterium and methanogenic archaea belonged to the dominant prokaryotes, and genes encoding components of the Wood-Ljungdahl pathway were identified.

Sinks and sources of anammox bacteria in a wastewater treatment plant – screening with qPCR

2018 ◽  
Vol 78 (2) ◽  
pp. 441-451
Author(s):  
Linda Kanders ◽  
Maike Beier ◽  
Regina Nogueira ◽  
Emma Nehrenheim
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plant ◽  
Quantitative Polymerase Chain Reaction ◽  
Treatment Plant ◽  
Anammox Bacteria ◽  
Reject Water ◽  
Start Up ◽  
Reliable Source ◽  
Polymerase Chain ◽  
Anammox Process

Abstract The deammonification process, which includes nitritation and anammox bacteria, is an energy-efficient nitrogen removal process. Starting up an anammox process in a wastewater treatment plant (WWTP) is still widely believed to require external seeding of anammox bacteria. To demonstrate the principle of a non-seeded anammox start-up, anammox bacteria in potential sources must be quantified. In this study, seven digesters, their substrates and reject water were sampled and quantitative polymerase chain reaction (qPCR) was used to quantify both total and viable anammox bacteria. The results show that mesophilic digesters fed with nitrifying sludge (with high sludge ages) can be classified as a reliable source of anammox bacteria. Sludge hygienization and dewatering of digestate reduce the amount of anammox bacteria by one to two orders of magnitude and can be considered as a sink. The sampled reject waters contained on average >4.0 × 104 copies mL−1 and the majority of these cells (>87%) were viable cells. Furthermore, plants with side-stream anammox treatment appear to have higher overall quantities of anammox bacteria than those without such treatment. The present study contributes to the development of sustainable strategies for both start-up of anammox reactors and the possibility of improving microbial management in WWTPs.

Increased nitrogen removal in existing volumes at Sundet wastewater treatment plant, Växjö

2014 ◽  
Vol 9 (2) ◽  
pp. 215-224 ◽  
Author(s):  
Anneli Andersson Chan ◽  
Niklas Johansson ◽  
Magnus Christensson
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Nitrogen Removal ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Full Scale ◽  
Biofilm Reactor ◽  
Main Stream ◽  
Ammonium Oxidation ◽  
N Removal

Many wastewater treatment plants need to improve their nitrogen removal due to stricter requirements and increasing loads. This often means larger bioreactor volumes, which can be very expensive and is sometimes impossible if space is limited. Therefore, there is a need for compact hybrid solutions that can increase capacity within existing volumes. Two full-scale demonstration projects using moving bed biofilm reactor (MBBR) technology has proven to be an efficient way to treat nitrogen in existing volumes at Sundet wastewater treatment plant in Växjö. Increased nitrification and denitrification capacity in parts of the main stream were demonstrated through the Hybas™ process, a combination of MBBR and activated sludge using the integrated fixed-film activated sludge technology. The ANITA™ Mox process, using autotrophic N-removal through anaerobic ammonium oxidation (anammox), provided high nitrogen removal for the sludge liquor. Data collected on-site for over a year are analyzed and compared with the performance of conventional treatment systems. These two full-scale demonstration projects have been a successful learning experience in identifying and correcting both process and operational issues, which may not have arisen at pilot scale. The set objectives in terms of nitrogen removal were met for both processes and design modifications have been identified that will improve future operation at Sundet WWTP.

scholarly journals Anaerobic Ammonium Oxidation: From Laboratory to Full-Scale Application

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Shou-Qing Ni ◽  
Jian Zhang
Keyword(s):  
Wastewater Treatment ◽  
Culture Medium ◽  
Enrichment Culture ◽  
Full Scale ◽  
The Other ◽  
Anammox Bacteria ◽  
Ammonium Oxidation ◽  
Start Up ◽  
Fast Start ◽  
Full Operation

From discovery in the early 1990s to completion of full-scale anammox reactor, it took almost two decades to uncover the secret veil of anammox bacteria. There were three milestones during the commercialization of anammox: the development of the first enrichment culture medium, the completion of the first commercial anammox reactor, and the fast start-up of full-scale anammox plant. Till now, the culture of anammox bacteria experienced a big progress through two general strategies: (a) to start up a reactor from scratch and (b) to seed the reactor with enriched anammox sludge. The first full-scale anammox reactor took 3.5 years to realize full operation using the first approach due to several reasons besides the lack of anammox sludge. On the other hand, the first Asian anammox reactor started up in two months, thanks to the availability of anammox seed. Along with the implementation of anammox plants, anammox eventually becomes the priority choice for ammonium wastewater treatment.

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