scholarly journals Anaerobic Ammonium Oxidation: From Laboratory to Full-Scale Application

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Shou-Qing Ni ◽  
Jian Zhang
Keyword(s):  
Wastewater Treatment ◽  
Culture Medium ◽  
Enrichment Culture ◽  
Full Scale ◽  
The Other ◽  
Anammox Bacteria ◽  
Ammonium Oxidation ◽  
Start Up ◽  
Fast Start ◽  
Full Operation

From discovery in the early 1990s to completion of full-scale anammox reactor, it took almost two decades to uncover the secret veil of anammox bacteria. There were three milestones during the commercialization of anammox: the development of the first enrichment culture medium, the completion of the first commercial anammox reactor, and the fast start-up of full-scale anammox plant. Till now, the culture of anammox bacteria experienced a big progress through two general strategies: (a) to start up a reactor from scratch and (b) to seed the reactor with enriched anammox sludge. The first full-scale anammox reactor took 3.5 years to realize full operation using the first approach due to several reasons besides the lack of anammox sludge. On the other hand, the first Asian anammox reactor started up in two months, thanks to the availability of anammox seed. Along with the implementation of anammox plants, anammox eventually becomes the priority choice for ammonium wastewater treatment.

scholarly journals Diversity, enrichment, and genomic potential of anaerobic methane- and ammonium-oxidizing microorganisms from a brewery wastewater treatment plant

2020 ◽  
Vol 104 (16) ◽  
pp. 7201-7212 ◽  
Author(s):  
Karin Stultiens ◽  
Maartje A.H.J. van Kessel ◽  
Jeroen Frank ◽  
Peter Fischer ◽  
Chris Pelzer ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plant ◽  
Oxidation Rate ◽  
Enrichment Culture ◽  
Treatment Plant ◽  
Marker Genes ◽  
Anammox Bacteria ◽  
Ammonium Oxidation ◽  
Brewery Wastewater ◽  
Activity Measurements

Abstract Anaerobic wastewater treatment offers several advantages; however, the effluent of anaerobic digesters still contains high levels of ammonium and dissolved methane that need to be removed before these effluents can be discharged to surface waters. The simultaneous anaerobic removal of methane and ammonium by denitrifying (N-damo) methanotrophs in combination with anaerobic ammonium-oxidizing (anammox) bacteria could be a potential solution to this challenge. After a molecular survey of a wastewater plant treating brewery effluent, indicating the presence of both N-damo and anammox bacteria, we started an anaerobic bioreactor with a continuous supply of methane, ammonium, and nitrite to enrich these anaerobic microorganisms. After 14 months of operation, a stable enrichment culture containing two types of ‘Candidatus Methylomirabilis oxyfera’ bacteria and two strains of ‘Ca. Brocadia’-like anammox bacteria was achieved. In this community, anammox bacteria converted 80% of the nitrite with ammonium, while ‘Ca. Methylomirabilis’ contributed to 20% of the nitrite consumption. The analysis of metagenomic 16S rRNA reads and fluorescence in situ hybridization (FISH) correlated well and showed that, after 14 months, ‘Ca. Methylomirabilis’ and anammox bacteria constituted approximately 30 and 20% of the total microbial community. In addition, a substantial part (10%) of the community consisted of Phycisphaera-related planctomycetes. Assembly and binning of the metagenomic sequences resulted in high-quality draft genome of two ‘Ca. Methylomirabilis’ species containing the marker genes pmoCAB, xoxF, and nirS and putative NO dismutase genes. The anammox draft genomes most closely related to ‘Ca. Brocadia fulgida’ included the marker genes hzsABC, hao, and hdh. Whole-reactor and batch anaerobic activity measurements with methane, ammonium, nitrite, and nitrate revealed an average anaerobic methane oxidation rate of 0.12 mmol h−1 L−1 and ammonium oxidation rate of 0.5 mmol h−1 L−1. Together, this study describes the enrichment and draft genomes of anaerobic methanotrophs from a brewery wastewater treatment plant, where these organisms together with anammox bacteria can contribute significantly to the removal of methane and ammonium in a more sustainable way. Key points • An enrichment culture containing both N-damo and anammox bacteria was obtained. • Simultaneous consumption of ammonia, nitrite, and methane under anoxic conditions. • In-depth metagenomic biodiversity analysis of inoculum and enrichment culture.

Activities and Metabolic Versatility of Distinct Anammox Bacteria in a Full-Scale Wastewater Treatment System

2021 ◽  
pp. 117763
Author(s):  
Yuchun Yang ◽  
Mohammad Azari ◽  
Craig W. Herbold ◽  
Meng Li ◽  
Huaihai Chen ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Full Scale ◽  
Treatment System ◽  
Anammox Bacteria ◽  
Wastewater Treatment System ◽  
Metabolic Versatility

Rapid start-up of one-stage deammonification MBBR without addition of external inoculum

2016 ◽  
Vol 74 (11) ◽  
pp. 2541-2550 ◽  
Author(s):  
Linda Kanders ◽  
Daniel Ling ◽  
Emma Nehrenheim
Keyword(s):  
Wastewater Treatment Plants ◽  
Full Scale ◽  
Anammox Bacteria ◽  
Operational Mode ◽  
Bacterial Populations ◽  
Reject Water ◽  
One Stage ◽  
Start Up ◽  
Laboratory Reactor ◽  
Set Up

In recent years, the anammox process has emerged as a useful method for robust and efficient nitrogen removal in wastewater treatment plants (WWTPs). This paper evaluates a one-stage deammonification (nitritation and anammox) start-up using carrier material without using anammox inoculum. A continuous laboratory-scale process was followed by full-scale operation with reject water from the digesters at Bekkelaget WWTP in Oslo, Norway. A third laboratory reactor was run in operational mode to verify the suitability of reject water from thermophilic digestion for the deammonification process. The two start-ups presented were run with indigenous bacterial populations, intermittent aeration and dilution, to favour growth of the anammox bacterial branches. Evaluation was done by chemical and fluorescence in situ hybridization analyses. The results demonstrate that anammox culture can be set up in a one-stage process only using indigenous anammox bacteria and that a full-scale start-up process can be completed in less than 120 days.

Fast start-up strategies of MBBR for mariculture wastewater treatment

2019 ◽  
Vol 248 ◽  
pp. 109267 ◽  
Author(s):  
Changwei Li ◽  
Jiawei Liang ◽  
Xiaochang Lin ◽  
Hong Xu ◽  
Musa Abubakar Tadda ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Start Up ◽  
Fast Start

scholarly journals Simultaneous Nitrite-Dependent Anaerobic Methane and Ammonium Oxidation Processes

2011 ◽  
Vol 77 (19) ◽  
pp. 6802-6807 ◽  
Author(s):  
Francisca A. Luesken ◽  
Jaime Sánchez ◽  
Theo A. van Alen ◽  
Janeth Sanabria ◽  
Huub J. M. Op den Camp ◽  
...  
Keyword(s):  
Enrichment Culture ◽  
Anammox Bacteria ◽  
Anaerobic Oxidation Of Methane ◽  
Ammonium Oxidation ◽  
Content Type ◽  
Oxidation Of Methane ◽  
Activity Measurements ◽  
Near Future ◽  
Damo Bacteria

ABSTRACTNitrite-dependent anaerobic oxidation of methane (n-damo) and ammonium (anammox) are two recently discovered processes in the nitrogen cycle that are catalyzed by n-damo bacteria, including “CandidatusMethylomirabilis oxyfera,” and anammox bacteria, respectively. The feasibility of coculturing anammox and n-damo bacteria is important for implementation in wastewater treatment systems that contain substantial amounts of both methane and ammonium. Here we tested this possible coexistence experimentally. To obtain such a coculture, ammonium was fed to a stable enrichment culture of n-damo bacteria that still contained some residual anammox bacteria. The ammonium supplied to the reactor was consumed rapidly and could be gradually increased from 1 to 20 mM/day. The enriched coculture was monitored by fluorescencein situhybridization and 16S rRNA andpmoAgene clone libraries and activity measurements. After 161 days, a coculture with about equal amounts of n-damo and anammox bacteria was established that converted nitrite at a rate of 0.1 kg-N/m3/day (17.2 mmol day−1). This indicated that the application of such a coculture for nitrogen removal may be feasible in the near future.

scholarly journals Enrichment of anaerobic ammonium oxidation (anammox) bacteria for short start-up of the anammox process: a review

2015 ◽  
Vol 57 (30) ◽  
pp. 13958-13978 ◽  
Author(s):  
Mumtazah Ibrahim ◽  
Norjan Yusof ◽  
Mohd Zulkhairi Mohd Yusoff ◽  
Mohd Ali Hassan
Keyword(s):  
Anaerobic Ammonium Oxidation ◽  
Anammox Bacteria ◽  
Ammonium Oxidation ◽  
Start Up ◽  
Anammox Process

scholarly journals Achieving fast start-up of anammox process by promoting the growth of anammox bacteria with FeS addition

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Chunzhen Zou ◽  
Beibei Guo ◽  
Xuming Zhuang ◽  
Liying Ren ◽  
Shou-Qing Ni ◽  
...  
Keyword(s):  
Microbial Community ◽  
Nitrogen Removal ◽  
Nitrite Reductase ◽  
Removal Rate ◽  
Anammox Bacteria ◽  
Gene Encoding ◽  
Start Up ◽  
Fast Start ◽  
The Difference ◽  
Anammox Process

Abstract The effects of FeS on nitrogen removal performance and microbial community of anammox process were studied. During the start-up period, the removal efficiencies of nitrite and total nitrogen were significantly improved by FeS. The addition of FeS increased the content of iron ions in the reactor and promoted the synthesis of heme c, which was involved in the formation of various enzymes. Compared with the control, the abundance of anammox bacteria in the FeS reactor was increased by 29%, and the expression level of the nirS gene (encoding cd1 type nitrite reductase containing heme) was nearly doubled. The content of nitrite reductase (ammonia-forming) in the community was increased by 26.4%. The difference in functional bacteria and enzyme contents in the microbial community resulted in a difference in nitrogen removal rate (NRR) between the two reactors. High-throughput results indicated that FeS increased the richness and diversity of microbial community and enhanced the metabolic function of the microbial community. The addition of FeS did not change the dominant position of Ca. Kuenenia in both reactors. But the relative abundance of heterotrophic denitrifying bacteria was reduced with FeS, which may be related to the inhibition effect of S2− produced by FeS.

New Technologies for Anaerobic Wastewater Treatment

1986 ◽  
Vol 18 (12) ◽  
pp. 41-53 ◽  
Author(s):  
Look Hulshoff Pol ◽  
Gatze Lettinga
Keyword(s):  
Wastewater Treatment ◽  
New Technologies ◽  
Anaerobic Treatment ◽  
Full Scale ◽  
Upflow Anaerobic Sludge Blanket ◽  
Anaerobic Sludge ◽  
Anaerobic Wastewater Treatment ◽  
Start Up ◽  
Uasb Reactors ◽  
Sludge Granulation

Presently anaerobic wastewater treatment is becoming an accepted simple technology for the treatment of a variety of wastewaters. Of the different treatment systems that have been developed the UASB process (Upflow Anaerobic Sludge Blanket) has found the widest application. Almost all of the more than 60 full scale UASB reactors in operation now, are running satisfactorily. The excellent sludge retention generally found in UASB-reactors is obtained by sludge granulation, which can be seen as a sludge immobilization process. The presently available insight into the sludge granulation process is briefly presented, together with the strategy to be applied for performing a proper first start-up and secondary start-up of UASB reactors, viz. using granular seed sludge. The effect of the presence of SS with regard to the loading potentials of anaerobic treatment systems will be discussed. The experiences obtained with some full scale applications of the UASB-process are presented.

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