Carbon nanomaterial properties help to enhance xylanase production from recombinant Kluyveromyces lactis through a cell immobilization method

2021 ◽  
Author(s):  
Shoriya Aruni Abdul Manaf ◽  
Siti Fatimah Zaharah Mohamad Fuzi ◽  
Kheng Oon Low ◽  
Gurumurthy Hegde ◽  
Nor Hasmaliana Abdul Manas ◽  
...  
Keyword(s):  
Kluyveromyces Lactis ◽  
Cell Immobilization ◽  
Carbon Nanomaterial ◽  
Xylanase Production ◽  
A Cell ◽  
Immobilization Method

scholarly journals Immobilization of fish chromatophores for use as a micro-biosensor for biological toxins

2003 ◽  
Vol 57 (12) ◽  
pp. 605-610 ◽  
Author(s):  
Ljiljana Mojovic ◽  
Goran Jovanovic
Keyword(s):  
Cell Attachment ◽  
Cell Immobilization ◽  
Betta Splendens ◽  
Microbial Pathogens ◽  
Fish Cell ◽  
First Order Kinetics ◽  
Microcarrier Bead ◽  
Color Response ◽  
A Cell ◽  
Selection Of

Chromatophores isolated from the Siamese fighting fish, Betta splendens represent a class of living cells that provide a vivid color response to microbial pathogens and environmental toxins. The selection of the most appropriate microcarrier and the development of the optimal technique for the chromatophore immobilization in order to enable directed transport of the sensor cells throughout microchannels of the biosensor, as well to preserve the cell survival and its functionality was studied. Microcarriers derived from glass, polystyrene and gelatin (collagen) were tested as substrates for chromatophore attachement. Gelatin microcarriers were found to be the most suitable, due to high attachment efficiency (95% of attached cells), preservation of the cell viability and enhanced cell sensitivity. The optimum conditions for fish cell immobilization on collagen microcarriers were determined based on the cell-to-microcarrier bead ratio and the pH of the solution. The rate of cell attachment to the gelatin microcarrier followed first-order kinetics. Pretreatment of the gelatin beads with fibronectin, known as a cell attachment-promoting agent, resulted in a 10% higher attachment rate constant (k).

Development and validation of a medium for recombinant endo-β-1,4-xylanase production by Kluyveromyces lactis using a statistical experimental design

2011 ◽  
Vol 62 (1) ◽  
pp. 283-292 ◽  
Author(s):  
Siti Fatimah Zaharah Mohamad Fuzi ◽  
Nor Muhammad Mahadi ◽  
Jamaliah Md. Jahim ◽  
Abdul Munir Abd. Murad ◽  
Farah Diba Abu Bakar ◽  
...  
Keyword(s):  
Experimental Design ◽  
Kluyveromyces Lactis ◽  
Statistical Experimental Design ◽  
Xylanase Production ◽  
Development And Validation

scholarly journals Development and Characterization of an Enzyme Membrane Reactor for Fructo-Oligosaccharide Production

Membranes ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 148 ◽  
Author(s):  
Burghardt ◽  
Coletta ◽  
van der Bolt ◽  
Ebrahimi ◽  
Gerlach ◽  
...  
Keyword(s):  
Membrane Reactor ◽  
Kluyveromyces Lactis ◽  
Calorific Value ◽  
Feed Additives ◽  
Efficient Production ◽  
Enzyme Membrane Reactor ◽  
Food And Feed ◽  
A Cell ◽  
Enzyme Molecules

Fructo-oligosaccharides (FOS) are linear fructans comprising 2–5 fructose units linked to a terminal glucose residue. They are widely used as food and feed additives due to their sweetness, low calorific value, and prebiotic properties. Here we describe the synthesis of FOS catalyzed by a cell-free crude enzyme solution containing recombinant fructosyltransferase (1-FFT) produced in the yeast Kluyveromyces lactis. During the enzyme catalysis, glucose accumulates as a by-product and eventually inhibits FOS production. We therefore used an enzyme membrane reactor (EMR) to achieve the continuous removal of glucose and the simultaneous replenishment of sucrose. We observed a loss of flux during the reaction with the characteristics of complete pore blocking, probably caused by a combination of proteins (enzyme molecules) and polysaccharides (FOS). Such complex fouling mechanisms must be overcome to achieve the efficient production of FOS using EMR systems.

scholarly journals Vapor-based micro/nano-partitioning of fluoro-functional group immobilization for long-term stable cell patterning

RSC Advances ◽  
2016 ◽  
Vol 6 (98) ◽  
pp. 96306-96313 ◽  
Author(s):  
Shun-ichi Funano ◽  
Nobuyuki Tanaka ◽  
Yo Tanaka
Keyword(s):  
Cell Culture ◽  
Functional Group ◽  
Cell Patterning ◽  
Nano Scale ◽  
A Cell ◽  
Culture Surface ◽  
Immobilization Method

This study developed a simple vapor-based immobilization method using a compound with fluoro-functional-group on a cell culture surface with micro/nano scale patterns.

Development of a cell immobilization technique with polyvinyl alcohol for diesel remediation in seawater

2016 ◽  
Vol 113 ◽  
pp. 397-407 ◽  
Author(s):  
Pao-Wen Grace Liu ◽  
Dun-Sheng Yang ◽  
Jyun-Yu Tang ◽  
Han-Wei Hsu ◽  
Chih-Hung Chen ◽  
...  
Keyword(s):  
Polyvinyl Alcohol ◽  
Cell Immobilization ◽  
A Cell

Simplified feeding strategies for the fed-batch cultivation of Kluyveromyces lactis GG799 for enhanced recombinant xylanase production

2014 ◽  
Vol 37 (9) ◽  
pp. 1887-1898 ◽  
Author(s):  
Siti Fatimah Zaharah Mohamad Fuzi ◽  
Firdausi Razali ◽  
Jamaliah Md. Jahim ◽  
Roshanida A. Rahman ◽  
Rosli Md. Illias
Keyword(s):  
Kluyveromyces Lactis ◽  
Batch Cultivation ◽  
Feeding Strategies ◽  
Fed Batch ◽  
Xylanase Production ◽  
Fed Batch Cultivation

Effect of Niacin on Mitochondria of Allium Cepa Root Cells

1967 ◽  
Vol 25 ◽  
pp. 78-79
Author(s):  
M. Arif Hayat
Keyword(s):  
Nicotinamide Adenine Dinucleotide ◽  
Hydrogen Transfer ◽  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
Nicotinamide Adenine ◽  
Root Tips ◽  
Root Cells ◽  
Transfer Processes ◽  
Standard Procedures ◽  
A Cell ◽  
Critical Interest

Although it is recognized that niacin (pyridine-3-carboxylic acid), incorporated as the amide in nicotinamide adenine dinucleotide (NAD) or in nicotinamide adenine dinucleotide phosphate (NADP), is a cofactor in hydrogen transfer in numerous enzyme reactions in all organisms studied, virtually no information is available on the effect of this vitamin on a cell at the submicroscopic level. Since mitochondria act as sites for many hydrogen transfer processes, the possible response of mitochondria to niacin treatment is, therefore, of critical interest.Onion bulbs were placed on vials filled with double distilled water in the dark at 25°C. After two days the bulbs and newly developed root system were transferred to vials containing 0.1% niacin. Root tips were collected at ¼, ½, 1, 2, 4, and 8 hr. intervals after treatment. The tissues were fixed in glutaraldehyde-OsO4 as well as in 2% KMnO4 according to standard procedures. In both cases, the tissues were dehydrated in an acetone series and embedded in Reynolds' lead citrate for 3-10 minutes.

Sign in / Sign up

Export Citation Format

Share Document