Characterization of a novel broad-spectrum endolysin PlyD4 encoded by a highly conserved prophage found in Aeromonas hydrophila ST251 strains

2022 ◽  
Author(s):  
Chaojie Wang ◽  
Shengchi Shi ◽  
Meiju Wei ◽  
Yi Luo
Keyword(s):  
Broad Spectrum ◽  
Aeromonas Hydrophila

scholarly journals Molecular and Biochemical Characterization of a Novel Class A β-Lactamase (HER-1) from Escherichia hermannii

2003 ◽  
Vol 47 (8) ◽  
pp. 2669-2673 ◽  
Author(s):  
Anne Beauchef-Havard ◽  
Guillaume Arlet ◽  
Valerie Gautier ◽  
Roger Labia ◽  
Patrick Grimont ◽  
...  
Keyword(s):  
Amino Acid ◽  
Broad Spectrum ◽  
Aeromonas Hydrophila ◽  
Biochemical Characterization ◽  
Amino Acid Identity ◽  
Acid Identity ◽  
Low Level ◽  
Class A ◽  
Moderate Susceptibility

ABSTRACT Escherichia hermannii showed a low level of resistance to amoxicillin and ticarcillin, reversed by clavulanate, and a moderate susceptibility to piperacillin but was susceptible to all cephalosporins. A bla gene was cloned and encoded a typical class A β-lactamase (HER-1, pI 7.5), which shares 45, 44, 41, and 40% amino acid identity with other β-lactamases, AER-1 from Aeromonas hydrophila, MAL-1/Cko-1 from Citrobacter koseri, and TEM-1 and LEN-1, respectively. No ampR gene was detected. Only penicillins were efficiently hydrolyzed, and no hydrolysis was observed for cefuroxime and broad-spectrum cephalosporins. Sequencing of the bla gene in 12 other strains showed 98 to 100% identity with bla HER-1.

Characterization of an SHV-5 related extended broad-spectrum beta-lactamase in enterobacteriaceae from western australia

Pathology ◽  
1993 ◽  
Vol 25 (1) ◽  
pp. 71-75 ◽  
Author(s):  
Leigh Mulgrave ◽  
Paul V. Attwood
Keyword(s):  
Western Australia ◽  
Broad Spectrum ◽  
Beta Lactamase

Molecular characterization of integron-gene cassettes in multi-drug resistant Aeromonas hydrophila from fish

2013 ◽  
Vol 20 (5) ◽  
pp. 1015-1022
Author(s):  
Shaowu LI ◽  
Di WANG ◽  
Hongbai LIU ◽  
Jiasheng YIN ◽  
Tongyan LU
Keyword(s):  
Molecular Characterization ◽  
Aeromonas Hydrophila ◽  
Drug Resistant ◽  
Gene Cassettes

Facile syringe filter-enabled bacteria separation, enrichment, and buffer exchange for clinical isolation-free digital detection and characterization of bacterial pathogens in urine

The Analyst ◽  
2021 ◽  
Author(s):  
Pengfei Zhang ◽  
Aniruddha Kaushik ◽  
Kathleen E Mach ◽  
Kuangwen Hsieh ◽  
Joseph C. Liao ◽  
...  
Keyword(s):  
Infectious Diseases ◽  
Antibiotic Therapy ◽  
Broad Spectrum ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Evidence Based ◽  
Pathogen Identification ◽  
Accelerated Methods ◽  
Syringe Filter

The development of accelerated methods for pathogen identification (ID) and antimicrobial susceptibility testing (AST) for infectious diseases is necessary to facilitate evidence-based antibiotic therapy and reduce clinical overreliance on broad-spectrum...

Characterization of histopathological and ultrastructural changes in Myxocyprinus asiaticus acutely infected with virulent Aeromonas hydrophila

2021 ◽  
Author(s):  
Fang Li ◽  
Haoran Gu ◽  
Fang Ou ◽  
Hailong Ge ◽  
Xiaohong Liu ◽  
...  
Keyword(s):  
Aeromonas Hydrophila ◽  
Ultrastructural Changes ◽  
Myxocyprinus Asiaticus ◽  
Virulent Aeromonas Hydrophila

scholarly journals Molecular and Functional Characterization of a ToxR-Regulated Lipoprotein from a Clinical Isolate of Aeromonas hydrophila

2006 ◽  
Vol 74 (7) ◽  
pp. 3742-3755 ◽  
Author(s):  
Lakshmi Pillai ◽  
Jian Sha ◽  
Tatiana E. Erova ◽  
Amin A. Fadl ◽  
Bijay K. Khajanchi ◽  
...  
Keyword(s):  
Virulence Factor ◽  
Aeromonas Hydrophila ◽  
Functional Characterization ◽  
Affinity Column ◽  
Serum Resistance ◽  
Classical Pathway ◽  
Dependent Manner ◽  
T7 Promoter

ABSTRACT Human diseases caused by species of Aeromonas have been classified into two major groups: septicemia and gastroenteritis. In this study, we reported the molecular and functional characterization of a new virulence factor, ToxR-regulated lipoprotein, or TagA, from a diarrheal isolate, SSU, of Aeromonas hydrophila. The tagA gene of A. hydrophila exhibited 60% identity with that of a recently identified stcE gene from Escherichia coli O157:H7, which encoded a protein (StcE) that provided serum resistance to the bacterium and prevented erythrocyte lysis by controlling classical pathway of complement activation by cleaving the complement C1-esterase inhibitor (C1-INH). We purified A. hydrophila TagA as a histidine-tagged fusion protein (rTagA) from E. coli DE3 strain using a T7 promoter-based pET30 expression vector and nickel affinity column chromatography. rTagA cleaved C1-INH in a time-dependent manner. The tagA isogenic mutant of A. hydrophila, unlike its corresponding wild-type (WT) or the complemented strain, was unable to cleave C1-INH, which is required to potentiate the C1-INH-mediated lysis of host and bacterial cells. We indeed demonstrated colocalization of C1-INH and TagA on the bacterial surface by confocal fluorescence microscopy, which ultimately resulted in increased serum resistance of the WT bacterium. Likewise, we delineated the role of TagA in contributing to the enhanced ability of C1-INH to inhibit the classical complement-mediated lysis of erythrocytes. Importantly, we provided evidence that the tagA mutant was significantly less virulent in a mouse model of infection (60%) than the WT bacterium at two 50% lethal doses, which resulted in 100% mortality within 48 h. Taken together, our data provided new information on the role of TagA as a virulence factor in bacterial pathogenesis. This is the first report of TagA characterization from any species of Aeromonas.

Occurrence and characterization of Aeromonas hydrophila and Yersinia enterocolitica in minimally processed fresh vegetable salads

Food Control ◽  
2010 ◽  
Vol 21 (4) ◽  
pp. 393-398 ◽  
Author(s):  
V. Xanthopoulos ◽  
N. Tzanetakis ◽  
Evanthia Litopoulou-Tzanetaki
Keyword(s):  
Yersinia Enterocolitica ◽  
Aeromonas Hydrophila ◽  
Minimally Processed ◽  
Fresh Vegetable

scholarly journals Kinetic and spectroscopic characterization of native and metal-substituted β-lactamase from Aeromonas hydrophila AE036

FEBS Letters ◽  
2000 ◽  
Vol 467 (2-3) ◽  
pp. 221-225 ◽  
Author(s):  
Maria Hernandez Valladares ◽  
Martin Kiefer ◽  
Uwe Heinz ◽  
Raquel Paul Soto ◽  
Wolfram Meyer-Klaucke ◽  
...  
Keyword(s):  
Aeromonas Hydrophila ◽  
Spectroscopic Characterization

Characterization of the ceruloplasmin gene and its potential role as an indirect marker for selection to Aeromonas hydrophila resistance in rohu, Labeo rohita

2013 ◽  
Vol 34 (5) ◽  
pp. 1325-1334 ◽  
Author(s):  
P.K. Sahoo ◽  
Sweta Das ◽  
Kanta Das Mahapatra ◽  
Jatindra Nath Saha ◽  
Matthew Baranski ◽  
...  
Keyword(s):  
Aeromonas Hydrophila ◽  
Potential Role ◽  
Labeo Rohita
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