Metabolic profiles and aprE expression in anaerobic cultures of Bacillus subtilis using nitrate as terminal electron acceptor

2001 ◽  
Vol 57 (3) ◽  
pp. 379-384 ◽  
Author(s):  
Espinosa-de-los-Monteros J. ◽  
Martinez A. ◽  
Valle F.
Keyword(s):  
Bacillus Subtilis ◽  
Electron Acceptor ◽  
Metabolic Profiles ◽  
Terminal Electron Acceptor

scholarly journals Biosurfactant production maintains viability in anoxic conditions by depolarizing the membrane in Bacillus subtilis

2019 ◽  
Author(s):  
Heidi A. Arjes ◽  
Lam Vo ◽  
Caroline Marie Dunn ◽  
Lisa Willis ◽  
Christopher A. DeRosa ◽  
...  
Keyword(s):  
Cell Wall ◽  
Bacillus Subtilis ◽  
Membrane Potential ◽  
Stationary Phase ◽  
Optical Density ◽  
Electron Acceptor ◽  
Oxygen Depletion ◽  
Aerobic Growth ◽  
Terminal Electron Acceptor ◽  
Obligate Aerobe

SummaryThe presence or absence of oxygen in the environment is a strong effector of cellular metabolism and physiology. Like many eukaryotes and some bacteria, Bacillus subtilis is an obligate aerobe that primarily utilizes oxygen during respiration to generate ATP. Despite the importance of oxygen for B. subtilis survival, we know little about how oxygen is consumed during growth and how populations respond to shifts in oxygen availability. Here, we find that when oxygen was depleted from stationary phase cultures ∼90% of B. subtilis 3610 cells died and lysed due to autolysin activity; the remaining cells maintained colony-forming ability. Interestingly, the domesticated 168 strain maintained a higher optical density than 3610 during oxygen depletion due to the formation of cell-wall-less protoplasts, but the remaining, rod-shaped cells were >100-fold less viable than 3610. We discovered that the higher viability in 3610 was due to its ability to produce the antibacterial compound surfactin, as surfactin addition rescued 168 viability and also increased yield in aerobic growth. We further demonstrate that surfactin strongly depolarizes the B. subtilis membrane, and that other known membrane-potential disruptors restore viability to 168. These findings highlight the importance of surfactin for survival during oxygen-depleted conditions and demonstrate that antimicrobials normally considered harmful can instead benefit cells in stressful conditions when the terminal electron acceptor in respiration is limiting.

Effects of terminal electron acceptor strength on film morphology and ternary memory performance of triphenylamine donor based devices

2013 ◽  
Vol 1 (24) ◽  
pp. 3816 ◽  
Author(s):  
Hao Zhuang ◽  
Qijian Zhang ◽  
Yongxiang Zhu ◽  
Xufeng Xu ◽  
Haifeng Liu ◽  
...  
Keyword(s):  
Electron Acceptor ◽  
Memory Performance ◽  
Film Morphology ◽  
Terminal Electron Acceptor

Magnetovibrio blakemorei gen. nov., sp. nov., a magnetotactic bacterium ( Alphaproteobacteria : Rhodospirillaceae ) isolated from a salt marsh

2013 ◽  
Vol 63 (Pt_5) ◽  
pp. 1824-1833 ◽  
Author(s):  
Dennis A. Bazylinski ◽  
Timothy J. Williams ◽  
Christopher T. Lefèvre ◽  
Denis Trubitsyn ◽  
Jiasong Fang ◽  
...  
Keyword(s):  
Salt Marsh ◽  
Electron Acceptor ◽  
Anaerobic Growth ◽  
Rrna Gene ◽  
Magnetotactic Bacterium ◽  
Single Chain ◽  
Circular Chromosome ◽  
Terminal Electron Acceptor ◽  
Content Type ◽  
Link Type

A magnetotactic bacterium, designated strain MV-1T, was isolated from sulfide-rich sediments in a salt marsh near Boston, MA, USA. Cells of strain MV-1T were Gram-negative, and vibrioid to helicoid in morphology. Cells were motile by means of a single polar flagellum. The cells appeared to display a transitional state between axial and polar magnetotaxis: cells swam in both directions, but generally had longer excursions in one direction than the other. Cells possessed a single chain of magnetosomes containing truncated hexaoctahedral crystals of magnetite, positioned along the long axis of the cell. Strain MV-1T was a microaerophile that was also capable of anaerobic growth on some nitrogen oxides. Salinities greater than 10 % seawater were required for growth. Strain MV-1T exhibited chemolithoautotrophic growth on thiosulfate and sulfide with oxygen as the terminal electron acceptor (microaerobic growth) and on thiosulfate using nitrous oxide (N2O) as the terminal electron acceptor (anaerobic growth). Chemo-organoautotrophic and methylotrophic growth was supported by formate under microaerobic conditions. Autotrophic growth occurred via the Calvin–Benson–Bassham cycle. Chemo-organoheterotrophic growth was supported by various organic acids and amino acids, under microaerobic and anaerobic conditions. Optimal growth occurred at pH 7.0 and 26–28 °C. The genome of strain MV-1T consisted of a single, circular chromosome, about 3.7 Mb in size, with a G+C content of 52.9–53.5 mol%.Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain MV-1T belongs to the family Rhodospirillaceae within the Alphaproteobacteria , but is not closely related to the genus Magnetospirillum . The name Magnetovibrio blakemorei gen. nov., sp. nov. is proposed for strain MV-1T. The type strain of Magnetovibrio blakemorei is MV-1T ( = ATCC BAA-1436T  = DSM 18854T).

Phasic availability of terminal electron acceptor on oxygen reduction reaction in microbial fuel cell

2017 ◽  
Vol 242 ◽  
pp. 101-108 ◽  
Author(s):  
J. Shanthi Sravan ◽  
Sai Kishore Butti ◽  
Anil Verma ◽  
S. Venkata Mohan
Keyword(s):  
Fuel Cell ◽  
Oxygen Reduction Reaction ◽  
Oxygen Reduction ◽  
Microbial Fuel Cell ◽  
Electron Acceptor ◽  
Reduction Reaction ◽  
Terminal Electron Acceptor

Rates and potential mechanism of anaerobic nitrate-dependent microbial pyrite oxidation

2012 ◽  
Vol 40 (6) ◽  
pp. 1280-1283 ◽  
Author(s):  
Julian Bosch ◽  
Rainer U. Meckenstock
Keyword(s):  
Electron Acceptor ◽  
Pyrite Oxidation ◽  
Laboratory Data ◽  
Laboratory Studies ◽  
Mineral Phase ◽  
Terminal Electron Acceptor ◽  
Micro Organisms ◽  
Open Question ◽  
Sulfur Containing ◽  
Positive Results

Pyrite (FeS2) is a major iron- and sulfur-containing mineral phase in the environment. Oxidation of pyrite by aerobic micro-organisms has been well investigated. However, the reactivity of pyrite under anoxic conditions is still an open question. In the present paper, we summarize field and laboratory data on this chemolithotrophic respiration process with nitrate as terminal electron acceptor. Geochemical and stable isotope field data indicate that this process is occurring. Laboratory studies are more ambiguous, but recent positive results provide evidence that anaerobic microbial pyrite oxidation can, in fact, occur with nitrate as electron acceptor.

Effect of ascorbate on oxygen uptake and growth of Escherichia coli B

1988 ◽  
Vol 34 (6) ◽  
pp. 822-824 ◽  
Author(s):  
Holly E. Richter ◽  
Jacek Switala ◽  
Peter C. Loewen
Keyword(s):  
Escherichia Coli ◽  
Oxygen Uptake ◽  
Electron Acceptor ◽  
Minimal Medium ◽  
Anaerobic Growth ◽  
Rich Medium ◽  
Terminal Electron Acceptor ◽  
Subsequent Change ◽  
Escherichia Coli B

The addition of ascorbate to aerobically growing cultures of Escherichia coli B caused only a short pause in growth and no subsequent change in the rate or extent of growth. The effect of ascorbate on oxygen uptake varied from inhibition in minimal medium to stimulation in rich medium. Cyanide-resistant growth and oxygen uptake were stimulated by ascorbate. Both the rate and extent of anaerobic growth were stimulated in proportion to the amount of ascorbate added when fumarate was the terminal electron acceptor. Ascorbate had no effect on any aspect of anaerobic growth in the absence of a terminal electron acceptor or in the presence of nitrate.

scholarly journals Geobacter sulfurreducens Can Grow with Oxygen as a Terminal Electron Acceptor

2004 ◽  
Vol 70 (4) ◽  
pp. 2525-2528 ◽  
Author(s):  
W. C. Lin ◽  
M. V. Coppi ◽  
D. R. Lovley
Keyword(s):  
Electron Acceptor ◽  
Atmospheric Oxygen ◽  
Geobacter Sulfurreducens ◽  
Terminal Electron Acceptor ◽  
Anoxic Conditions ◽  
Strict Anaerobe ◽  
Subsurface Environments

ABSTRACT Geobacter sulfurreducens, previously classified as a strict anaerobe, tolerated exposure to atmospheric oxygen for at least 24 h and grew with oxygen as the sole electron acceptor at concentrations of 10% or less in the headspace. These results help explain how Geobacter species may survive in oxic subsurface environments, being poised to rapidly take advantage of the development of anoxic conditions.

Anaerobic Degradation of Toluene and Xylene—Evidence for Sulphate as the Terminal Electron Acceptor

1991 ◽  
pp. 463-471 ◽  
Author(s):  
E.A. Edwards ◽  
L.E. Wills ◽  
D. Grbić-Galić ◽  
M. Reinhard
Keyword(s):  
Electron Acceptor ◽  
Anaerobic Degradation ◽  
Terminal Electron Acceptor
Sign in / Sign up

Export Citation Format

Share Document