Two experiments were conducted on Acer rubrum L. to determine the influence of root severance on sap flow, stomatal conductance, leaf water potential (ψ), and stem xylem embolism. Experiment 1 utilized 3-year-old trees, and experiment 2 utilized 2-year-old trees. Sixteenmm sap flow gauges were installed on both groups. Trees for experiment 1 were harvested on 31 May 1996 with a root ball diameter of 30.5 cm. Sap flow was reduced within one day after plants were harvested and was still lower 1 week after harvest. On 7 June 1996, harvested trees had lower stomatal conductance measurements, compared to not-harvested trees, but ψ were similar. A second experiment was initiated on 20 Aug. 1996, using the same protocol as in experiment 1. Sap flow was reduced within 2 h after harvest for harvested trees compared to not-harvested trees. Leaf stomatal conductances were reduced within 4 h of harvest. Leaf water potentials were not influenced on the day that the trees were harvested. Embolism levels were increased by harvest within 24 h. These results indicate that transplant stress begins shortly after harvest and not at the actual time of transplant.