Differential Proteome Between Patient-Related and Non-related Environmental Isolates of Legionella pneumophila

2017 ◽  
Vol 74 (3) ◽  
pp. 344-355
Author(s):  
Sara Quero ◽  
Marian García-Núñez ◽  
Noemí Párraga-Niño ◽  
M. Luisa Pedro-Botet ◽  
Lourdes Mateu ◽  
...  
Keyword(s):  
Legionella Pneumophila ◽  
Environmental Isolates

scholarly journals Lessons From an Outbreak of Legionnaires’ Disease on a Hematology-Oncology Unit

2016 ◽  
Vol 38 (3) ◽  
pp. 306-313 ◽  
Author(s):  
Louise K. Francois Watkins ◽  
Karrie-Ann E. Toews ◽  
Aaron M. Harris ◽  
Sherri Davidson ◽  
Stephanie Ayers-Millsap ◽  
...  
Keyword(s):  
Legionella Pneumophila ◽  
Potable Water ◽  
Single Case ◽  
Healthcare Providers ◽  
Water System ◽  
Positive Sequence ◽  
Environmental Isolates ◽  
Legionnaires Disease ◽  
Oncology Unit ◽  
Healthcare Associated

OBJECTIVESTo define the scope of an outbreak of Legionnaires’ disease (LD), to identify the source, and to stop transmission.DESIGN AND SETTINGEpidemiologic investigation of an LD outbreak among patients and a visitor exposed to a newly constructed hematology-oncology unit.METHODSAn LD case was defined as radiographically confirmed pneumonia in a person with positive urinary antigen testing and/or respiratory culture forLegionellaand exposure to the hematology-oncology unit after February 20, 2014. Cases were classified as definitely or probably healthcare-associated based on whether they were exposed to the unit for all or part of the incubation period (2–10 days). We conducted an environmental assessment and collected water samples for culture. Clinical and environmental isolates were compared by monoclonal antibody (MAb) and sequence-based typing.RESULTSOver a 12-week period, 10 cases were identified, including 6 definite and 4 probable cases. Environmental sampling revealedLegionella pneumophilaserogroup 1 (Lp1) in the potable water at 9 of 10 unit sites (90%), including all patient rooms tested. The 3 clinical isolates were identical to environmental isolates from the unit (MAb2-positive, sequence type ST36). No cases occurred with exposure after the implementation of water restrictions followed by point-of-use filters.CONCLUSIONSContamination of the unit’s potable water system with Lp1 strain ST36 was the likely source of this outbreak. Healthcare providers should routinely test patients who develop pneumonia at least 2 days after hospital admission for LD. A single case of LD that is definitely healthcare associated should prompt a full investigation.Infect Control Hosp Epidemiol2017;38:306–313

Genetic variability in environmental isolates of Legionella pneumophila from Comunidad Valenciana (Spain)

2006 ◽  
Vol 8 (6) ◽  
pp. 1056-1063 ◽  
Author(s):  
Mireia Coscolla ◽  
Maria Jose Gosalbes ◽  
Vicente Catalan ◽  
Fernando Gonzalez-Candelas
Keyword(s):  
Genetic Variability ◽  
Legionella Pneumophila ◽  
Environmental Isolates

scholarly journals Complete Genome Sequences of Six Legionella pneumophila Isolates from Two Collocated Outbreaks of Legionnaires’ Disease in 2005 and 2008 in Sarpsborg/Fredrikstad, Norway

2016 ◽  
Vol 4 (6) ◽  
Author(s):  
Marius Dybwad ◽  
Tone Aarskaug ◽  
Else-Marie Fykse ◽  
Elisabeth Henie Madslien ◽  
Janet Martha Blatny
Keyword(s):  
Comparative Genomics ◽  
Genetic Relationship ◽  
Legionella Pneumophila ◽  
Complete Genome ◽  
Sequence Similarity ◽  
Secretion Systems ◽  
Environmental Isolates ◽  
Genome Sequences ◽  
High Sequence Similarity ◽  
Legionnaires Disease

Here, we report the complete genome sequences of Legionella pneumophila isolates from two collocated outbreaks of Legionnaires’ disease in 2005 and 2008 in Sarpsborg/Fredrikstad, Norway. One clinical and two environmental isolates were sequenced from each outbreak. The genome of all six isolates consisted of a 3.36 Mb-chromosome, while the 2005 genomes featured an additional 68 kb-episome sharing high sequence similarity with the L. pneumophila Lens plasmid. All six genomes contained multiple mobile genetic elements including novel combinations of type-IVA secretion systems. A comparative genomics study will be launched to resolve the genetic relationship between the L. pneumophila isolates.

scholarly journals Molecular Epidemiology ofLegionella pneumophilaInfection at a Canadian Tertiary Care Institution

1994 ◽  
Vol 5 (4) ◽  
pp. 157-162 ◽  
Author(s):  
LE Nicolle ◽  
H Bialkowska-Hobrzanska ◽  
B Dyck ◽  
L Sekla ◽  
S Parker
Keyword(s):  
Monoclonal Antibody ◽  
Molecular Epidemiology ◽  
Legionella Pneumophila ◽  
Tertiary Care ◽  
Environmental Isolates ◽  
Tertiary Care Centre ◽  
Clonal Type ◽  
Serotype 1 ◽  
Single Clone ◽  
Hospital Acquired

Objective: To characterize the molecular epidemiology ofLegionellaspecies infection at one Canadian tertiary care centre.Design: Twenty-eight clinical isolates and 12 environmental isolates obtained over a six-year period were analyzed by restriction fragment length polymorphism (rflp) of chromosomaldna. Isolates included 15 from 12 patients with hospital acquired illness and 13 from nine patients with community acquired infection.Results: One nosocomial strain wasLegionella micdadeiand one community strain wasLegionella pneumophilaserotype 6; all others wereL pneumophilaserotype 1.rflptyping revealed one clone for all cases of a 1985 single-ward outbreak and five of six nonoutbreakL pneumophilanosocomial cases. Anrflppattern identical or highly related to that of the nosocomial clonal type was noted among nine of 12L pneumophilaserotype 1 community isolates. The remaining three isolates had two relatedrflppatterns distinct from the institutional strain. The nosocomial and community strains were isolated from multiple institutional water samples in the institution. For the environmental isolates, monoclonal antibody typing was more discriminating thanrflptyping: seven monoclonal antibody subtypes were distinguished among 12 environmental isolates comprising three distinctrflppatterns.Conclusions: Despite multipleL pneumophilaserotype 1 strains isolated in the authors’ institutional water, a single clone ofL pneumophilaproduced most disease. Community acquired disease was caused by a wider variety of strains.

scholarly journals Nosocomial legionella pneumonia: demonstration of potable water as the source of infection

1988 ◽  
Vol 101 (3) ◽  
pp. 647-654 ◽  
Author(s):  
B. Ruf ◽  
D. Schürmann ◽  
I. Horbrach ◽  
K. Seodel ◽  
H. D. Pohle
Keyword(s):  
Monoclonal Antibodies ◽  
Water Supply ◽  
Legionella Pneumophila ◽  
Water Samples ◽  
Potable Water ◽  
Supply System ◽  
University Hospital ◽  
Environmental Isolates ◽  
Legionella Pneumonia ◽  
Source Of Infection

SUMMARYFrom January 1983 until December 1985, 35 cases of sporadic nosocomial legionella pneumonia, all caused byLegionella pneumophila, were diagnosed in a university hospital.L. pneumophilaserogroup (SG) 1 was cultured from 12 of the 35 cases and compared to correspondingL. pneumophilaSG 1 isolates from water outlets in the patients' immediate environment by subtyping with monoclonal antibodies. The corresponding environmental isolates were identical to 9 out of 12 (75%) of those from the cases. However, even in the remaining three cases identical subtypes were found distributed throughout the hospital water supply. From the hospital water supply four different subtypes ofL. pneumophilaSG 1 were isolated, three of which were implicated in legionella pneumonia. Of 453 water samples taken during the study 298 (65.8%) were positive for legionellae. Species ofLegionellaother thanL. pneumophilahave not been isolated. This may explain the exclusiveness ofL.pneumophilaas the legionella pneumonia-causing agent. Our results suggest that the water supply system was the source of infection.

scholarly journals Legionella pneumophilaand OtherLegionellaSpecies Isolated from Legionellosis Patients in Japan between 2008 and 2016

2018 ◽  
Vol 84 (18) ◽  
Author(s):  
Junko Amemura-Maekawa ◽  
Fumiaki Kura ◽  
Kyoko Chida ◽  
Hitomi Ohya ◽  
Jun-ichi Kanatani ◽  
...  
Keyword(s):  
Legionella Pneumophila ◽  
Minimum Spanning Tree ◽  
Clinical Isolates ◽  
Small Scale ◽  
Environmental Isolates ◽  
Content Type ◽  
Suspected Infection ◽  
Source Of Infection ◽  
Infection Source ◽  
Bath Water

ABSTRACTTheLegionellaReference Center in Japan collected 427Legionellaclinical isolates between 2008 and 2016, including 7 representative isolates from corresponding outbreaks. The collection included 419Legionella pneumophilaisolates, of which 372 belonged to serogroup 1 (SG1) (87%) and the others belonged to SG2 to SG15 except for SG7 and SG11, and 8 isolates of otherLegionellaspecies (Legionella bozemanae,Legionella dumoffii,Legionella feeleii,Legionella longbeachae,Legionella londiniensis, andLegionella rubrilucens).L. pneumophilaisolates were genotyped by sequence-based typing (SBT) and represented 187 sequence types (STs), of which 126 occurred in a single isolate (index of discrimination of 0.984). These STs were analyzed using minimum spanning tree analysis, resulting in the formation of 18 groups. The pattern of overall ST distribution amongL. pneumophilaisolates was diverse. In particular, some STs were frequently isolated and were suggested to be related to the infection sources. The major STs were ST23 (35 isolates), ST120 (20 isolates), and ST138 (16 isolates). ST23 was the most prevalent and most causative ST for outbreaks in Japan and Europe. ST138 has been observed only in Japan, where it has caused small-scale outbreaks; 81% of those strains (13 isolates) were suspected or confirmed to infect humans through bath water sources. On the other hand, 11 ST23 strains (31%) and 5 ST120 strains (25%) were suspected or confirmed to infect humans through bath water. These findings suggest that some ST strains frequently cause legionellosis in Japan and are found under different environmental conditions.IMPORTANCELegionella pneumophilaserogroup 1 (SG1) is the most frequent cause of legionellosis. Our previous genetic analysis indicated that SG1 environmental isolates represented 8 major clonal complexes, consisting of 3 B groups, 2 C groups, and 3 S groups, which included major environmental isolates derived from bath water, cooling towers, and soil and puddles, respectively. Here, we surveyed clinical isolates collected from patients with legionellosis in Japan between 2008 and 2016. Most strains belonging to the B group were isolated from patients for whom bath water was the suspected or confirmed source of infection. Among the isolates derived from patients whose suspected infection source was soil or dust, most belonged to the S1 group and none belonged to the B or C groups. Additionally, the U group was discovered as a new group, which mainly included clinical isolates with unknown infection sources.

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