scholarly journals Molecular Epidemiology ofLegionella pneumophilaInfection at a Canadian Tertiary Care Institution

1994 ◽  
Vol 5 (4) ◽  
pp. 157-162 ◽  
Author(s):  
LE Nicolle ◽  
H Bialkowska-Hobrzanska ◽  
B Dyck ◽  
L Sekla ◽  
S Parker
Keyword(s):  
Monoclonal Antibody ◽  
Molecular Epidemiology ◽  
Legionella Pneumophila ◽  
Tertiary Care ◽  
Environmental Isolates ◽  
Tertiary Care Centre ◽  
Clonal Type ◽  
Serotype 1 ◽  
Single Clone ◽  
Hospital Acquired

Objective: To characterize the molecular epidemiology ofLegionellaspecies infection at one Canadian tertiary care centre.Design: Twenty-eight clinical isolates and 12 environmental isolates obtained over a six-year period were analyzed by restriction fragment length polymorphism (rflp) of chromosomaldna. Isolates included 15 from 12 patients with hospital acquired illness and 13 from nine patients with community acquired infection.Results: One nosocomial strain wasLegionella micdadeiand one community strain wasLegionella pneumophilaserotype 6; all others wereL pneumophilaserotype 1.rflptyping revealed one clone for all cases of a 1985 single-ward outbreak and five of six nonoutbreakL pneumophilanosocomial cases. Anrflppattern identical or highly related to that of the nosocomial clonal type was noted among nine of 12L pneumophilaserotype 1 community isolates. The remaining three isolates had two relatedrflppatterns distinct from the institutional strain. The nosocomial and community strains were isolated from multiple institutional water samples in the institution. For the environmental isolates, monoclonal antibody typing was more discriminating thanrflptyping: seven monoclonal antibody subtypes were distinguished among 12 environmental isolates comprising three distinctrflppatterns.Conclusions: Despite multipleL pneumophilaserotype 1 strains isolated in the authors’ institutional water, a single clone ofL pneumophilaproduced most disease. Community acquired disease was caused by a wider variety of strains.

Biclonal Multiple Myeloma- A Case Series

2021 ◽  
Author(s):  
Somnath Roy ◽  
Satvik Khaddar ◽  
Amit Agrawal ◽  
Geeta Rathnakumar ◽  
Lingaraj Nayak ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Plasma Cell ◽  
Tertiary Care ◽  
Case Series ◽  
Standard Chemotherapy ◽  
Tertiary Care Centre ◽  
Single Clone ◽  
Immunofixation Electrophoresis ◽  
Plasma Cell Dyscrasias ◽  
Biclonal Gammopathy

Abstract Multiple myeloma is a prototype of plasma cell dyscrasias characterized by monoclonal abnormal proliferation of immunoglobulin secreting plasma cell in the bone marrow ; resulting in production of monoclonal (M) protein (IgG,IgA,IgM,IgD) and or light chain concentrations (kappa or lamda) identified by protein electrophoresis and or immunofixation of serum or urine. The term biclonal multiple myeloma are defined by coexistence of two different M components, which could be either from a single clone or two separate clones producing two distinct bands in electrophoresis and or immunofixation of serum or urine. Biclonal gammopathy is a rare entity with upto 1% of newly diagnosed case of multiple myeloma have two M component in serum immunofixation electrophoresis. Here we share our experience of four cases of biclonal myeloma successfully diagnosed and treated with standard chemotherapy with satisfactory clinical outcome from a single tertiary care centre.

scholarly journals It Is Not All about Single Nucleotide Polymorphisms: Comparison of Mobile Genetic Elements and Deletions in Listeria monocytogenes Genomes Links Cases of Hospital-Acquired Listeriosis to the Environmental Source

2015 ◽  
Vol 53 (11) ◽  
pp. 3492-3500 ◽  
Author(s):  
Qinning Wang ◽  
Nadine Holmes ◽  
Elena Martinez ◽  
Peter Howard ◽  
Grant Hill-Cawthorne ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Genomic Analysis ◽  
Comparative Genomic ◽  
Snp Analysis ◽  
Nucleotide Polymorphisms ◽  
Environmental Isolates ◽  
Single Nucleotide ◽  
Content Type ◽  
Serotype 1 ◽  
Hospital Acquired

The control of food-borne outbreaks caused byListeria monocytogenesin humans relies on the timely identification of food or environmental sources and the differentiation of outbreak-related isolates from unrelated ones. This study illustrates the utility of whole-genome sequencing for examining the link between clinical and environmental isolates ofL. monocytogenesassociated with an outbreak of hospital-acquired listeriosis in Sydney, Australia. Comparative genomic analysis confirmed an epidemiological link between the three clinical and two environmental isolates. Single nucleotide polymorphism (SNP) analysis showed that only two SNPs separated the three human outbreak isolates, which differed by 19 to 20 SNPs from the environmental isolates and 71 to >10,000 SNPs from sporadicL. monocytogenesisolates. The chromosomes of all human outbreak isolates and the two suspected environmental isolates were syntenic. In contrast to the genomes of background sporadic isolates, all epidemiologically linked isolates contained two novel prophages and a previously unreported clustered regularly interspaced short palindromic repeat (CRISPR)-associated (Cas) locus subtype sequence. The mobile genetic element (MGE) profile of these isolates was distinct from that of the other serotype 1/2b reference strains and sporadic isolates. The identification of SNPs and clonally distinctive MGEs strengthened evidence to distinguish outbreak-related isolates ofL. monocytogenesfrom cocirculating endemic strains.

scholarly journals Emerging multidrug resistance isolates of hospital-acquired bacterial meningitis in a tertiary care centre in North India

2019 ◽  
Vol 68 (11) ◽  
pp. 1585-1590 ◽  
Author(s):  
M. S. Raza ◽  
B. K. Das ◽  
V. Goyal ◽  
R. Lodha ◽  
R. Chaudhry ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Bacterial Meningitis ◽  
Tertiary Care ◽  
North India ◽  
Care Centre ◽  
Tertiary Care Centre ◽  
Hospital Acquired

scholarly journals Clinical spectrum and risk factors for hospital-acquired septicemia in a tertiary care centre of North-East India

2020 ◽  
Vol 9 (8) ◽  
pp. 3949
Author(s):  
ShyamKishor Kumar ◽  
Ankita Kabi ◽  
Aroop Mohanty ◽  
Vanya Singh ◽  
MithileshKumar Jha ◽  
...  
Keyword(s):  
Risk Factors ◽  
Tertiary Care ◽  
Clinical Spectrum ◽  
Care Centre ◽  
Tertiary Care Centre ◽  
North East India ◽  
North East ◽  
Hospital Acquired

scholarly journals Legionella pneumophila: monoclonal antibody typing of clinical and environmental isolates

1987 ◽  
Vol 99 (2) ◽  
pp. 235-239 ◽  
Author(s):  
R. J. Brindle ◽  
P. J. Stannett ◽  
J. O'H. Tobin
Keyword(s):  
Monoclonal Antibody ◽  
Legionella Pneumophila ◽  
Clinical Isolates ◽  
Southern Europe ◽  
Environmental Isolates ◽  
Foreign Travel ◽  
Legionella Pneumonia ◽  
The United Kingdom ◽  
History Of ◽  
Sporadic Cases

SUMMARYForty-one clinical isolates ofLegionella pneumophilafrom sporadic cases of legionella pneumonia were collected from laboratories throughout the United Kingdom and were compared with 300 routine environmental isolates using two panels of monoclonal antibodies, covering serogroups 1–10. Eighty-five per cent of the clinical isolates belonged to the subgroup Pontiac of serogroup 1, whilst only 13% of the environmental isolates did. Approximately half of the clinical isolates tested came from patients with a recent history of foreign travel, mainly to southern Europe.

scholarly journals Molecular surveillance of carbapenemase-producing Pseudomonas aeruginosa at three medical centres in Cologne, Germany

2019 ◽  
Vol 8 (1) ◽  
Author(s):  
Elena Schäfer ◽  
Monika Malecki ◽  
Carlos J. Tellez-Castillo ◽  
Niels Pfennigwerth ◽  
Lennart Marlinghaus ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Intensive Care ◽  
Intensive Care Units ◽  
Tertiary Care ◽  
Antibiotic Resistance Genes ◽  
Carbapenem Resistance ◽  
Control Measures ◽  
Tertiary Care Centre ◽  
Molecular Surveillance ◽  
Hospital Acquired

Abstract Background Pseudomonas aeruginosa is a common pathogen causing hospital-acquired infections. Carbapenem resistance in P. aeruginosa is either mediated via a combination of efflux pumps, AmpC overexpression, and porin loss, or through an acquired carbapenemase. Carbapenemase-producing P. aeruginosa (CPPA) strains are known to cause outbreaks and harbour a reservoir of mobile antibiotic resistance genes, however, few molecular surveillance data is available. The aim of this study was to analyse the prevalence and epidemiology of CPPA in three German medical centres from 2015 to 2017. Methods Identification and susceptibility testing were performed with VITEK 2 system. P. aeruginosa non-susceptible to piperacillin, ceftazidime, cefepime, imipenem, meropenem and ciprofloxacin (4MRGN according to the German classification guideline) isolated from 2015 to 2017 were analysed. A two-step algorithm to detect carbapenemases was performed: phenotypic tests (EDTA- and cloxacillin-combined disk tests) followed by PCR, Sanger sequencing, and eventually whole genome sequencing. CPPA isolates were further genotyped by RAPD and PFGE. In-hospital transmission was investigated using conventional epidemiology. Results Sixty two P. aeruginosa isolates were available for further analysis, of which 21 were CPPA as follows: blaVIM-1 (n = 2), blaVIM-2 (n = 17), blaNDM-1/blaGES-5 (n = 1) and the newly described blaIMP-82 (n = 1). CPPA were mostly hospital-acquired (71.4%) and isolated on intensive care units (66.7%). All (except one) were from the tertiary care centre. PFGE typing revealed one large cluster of VIM-2-producing CPPA containing 13 isolates. However, using conventional epidemiology, we were only able to confirm three patient-to-patient transmissions, and one room-to-patient transmission, on several intensive care units. Conclusions These data give insight into the epidemiology of CPPA in three centres in Germany over a period of 3 years. Carbapenemases are a relevant resistance mechanism in 4MRGN-P. aeruginosa, illustrated by genetically related VIM-2-producing strains that seem to be endemic in this region. Our data suggest that infection control measures should especially focus on controlling transmission on the ICU and support the need for a local molecular surveillance system.

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