scholarly journals Correlations Between Root Metabolomics and Bacterial Community Structures in the Phragmites australis Under Acid Mine Drainage-Polluted Wetland Ecosystem

2021 ◽  
Vol 79 (1) ◽  
Author(s):  
Chimdi M. Kalu ◽  
Henry J. O. Ogola ◽  
Ramganesh Selvarajan ◽  
Memory Tekere ◽  
Khayalethu Ntushelo
Keyword(s):  
Acid Mine Drainage ◽  
Bacterial Community ◽  
Plant Growth ◽  
Phragmites Australis ◽  
Mine Drainage ◽  
Critical Role ◽  
Adenosine Monophosphate ◽  
Metagenomic Sequencing ◽  
Symbiotic Associations ◽  
Acid Mine

AbstractDespite root microecology playing critical role in plant growth and fidelity, relatively few studies have focused on the link between the microbial communities and root metabolome in the aquatic macrophytes under heavy metal (HM) pollution. Using high-throughput metagenomic sequencing, targeted metabolomics and community-level physiological profile analyses, we investigated the symbiotic associations between Phragmites australis with rhizospheric bacterial communities under differing acid mine drainage (AMD) pollution. Results indicated that AMD pollution and root localization significantly affected root metabolome profiles. Higher accumulation of adenosine monophosphate, inosine, methionine, carnitine and dimethylglycine were observed in the rhizosphere under AMD than non-AMD habitat. Overall, the bacterial diversity and richness, and functional (metabolic) diversity were lower under high-AMD pollution. While non-AMD site was enriched with members of phylum Firmicutes, Proteobacteria were the most abundant taxa in the rhizosphere and endosphere under AMD-polluted sites. Further, plant growth promoting rhizobacteria (Rhizobium, Delftia, Bradyrhizobium, and Mesorhizobium) and metal-tolerant bacteria (Bacillus, Arthrobacter, Massilia and Methylocystis) were most abundant in AMD-polluted than non-AMD habitat. Finally, pH, TDS (total dissolved solids), Cu, Cr, Fe, and Zn content were the key environmental factors that strongly contributed to the spatial perturbation of rhizospheric metabolites, proteobacterial and acidobacterial taxa. Overall, the study linked the differential endospheric and rhizospheric bacterial community and metabolite profiles in P. australis under AMD environment and provided insights into HM adaptability and phytoremediation potential.

scholarly journals Estimating Arsenic Mobility and Phytotoxicity Using Two Different Phosphorous Fertilizer Release Rates in Soil

Agronomy ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 111 ◽  
Author(s):  
Min-Suk Kim ◽  
Hyun-Gi Min ◽  
Jeong-Gyu Kim ◽  
Sang-Ryong Lee
Keyword(s):  
Acid Mine Drainage ◽  
Plant Growth ◽  
Contaminated Soil ◽  
Mine Drainage ◽  
Water Soluble ◽  
Single Treatment ◽  
Bone Meal ◽  
Acid Mine ◽  
Phytotoxicity Test ◽  
P Fertilizer

Deficiencies in phosphorus (P), an essential factor for plant growth and aided phytostabilization, are commonly observed in soil, especially near mining areas. The objective of this study was to compare the effect of P-based fertilizer types on arsenic (As) extractability and phytotoxicity in As-contaminated soil after stabilizer treatment. Different treatments with respect to the P-releasing characteristics were applied to soil to determine As mobility and phytotoxicity in P-based fertilizers, with bone meal as a slow-releasing P fertilizer and fused superphosphate as a fast-releasing P fertilizer. In addition, P fertilizers were used to enhance plant growth, and two types of iron (Fe)-based stabilizers (steel slang and acid mine drainage sludge) were also used to reduce As mobility in As-contaminated soil under lab-scale conditions. A water-soluble extraction was conducted to determine As and P extractability. A phytotoxicity test using bok choy (Brassica campestris L. ssp. chinensis Jusl.) was performed to assess the elongation and accumulation of As and P. Within a single treatment, the As stabilization was higher in steel slag (84%) than in acid mine drainage sludge (27%), and the P supply effect was higher in fused superphosphate (24740%) than in bone meal (160%) compared to the control. However, a large dose of fused superphosphate (2%) increased not only the water-soluble P, but also the water-soluble As, and consequently, increased As uptake by bok choy roots, leading to phytotoxicity. In combined treatments, the tendency towards change was similar to that of the single treatment, but the degree of change was decreased compared to the single treatment, thereby decreasing the risk of phytotoxicity. In particular, the toxicity observed in the fused superphosphate treatments did not appear in the bone meal treatment, but rather the growth enhancement effect appeared. These results indicate that the simultaneous application of bone meal and stabilizers might be proposed and could effectively increase plant growth via the stabilization of As and supplementation with P over the long term.

scholarly journals Efficient Low-pH Iron Removal by a Microbial Iron Oxide Mound Ecosystem at Scalp Level Run

2017 ◽  
Vol 83 (7) ◽  
Author(s):  
Christen L. Grettenberger ◽  
Alexandra R. Pearce ◽  
Kyle J. Bibby ◽  
Daniel S. Jones ◽  
William D. Burgos ◽  
...  
Keyword(s):  
Acid Mine Drainage ◽  
Bacterial Community ◽  
Microbial Communities ◽  
Mine Drainage ◽  
Iron Oxidation ◽  
Cost Effective ◽  
Low Ph ◽  
Content Type ◽  
Oxidation Rates ◽  
Acid Mine

ABSTRACT Acid mine drainage (AMD) is a major environmental problem affecting tens of thousands of kilometers of waterways worldwide. Passive bioremediation of AMD relies on microbial communities to oxidize and remove iron from the system; however, iron oxidation rates in AMD environments are highly variable among sites. At Scalp Level Run (Cambria County, PA), first-order iron oxidation rates are 10 times greater than at other coal-associated iron mounds in the Appalachians. We examined the bacterial community at Scalp Level Run to determine whether a unique community is responsible for the rapid iron oxidation rate. Despite strong geochemical gradients, including a >10-fold change in the concentration of ferrous iron from 57.3 mg/liter at the emergence to 2.5 mg/liter at the base of the coal tailings pile, the bacterial community composition was nearly constant with distance from the spring outflow. Scalp Level Run contains many of the same taxa present in other AMD sites, but the community is dominated by two strains of Ferrovum myxofaciens, a species that is associated with high rates of Fe(II) oxidation in laboratory studies. IMPORTANCE Acid mine drainage pollutes more than 19,300 km of rivers and streams and 72,000 ha of lakes worldwide. Remediation is frequently ineffective and costly, upwards of $100 billion globally and nearly $5 billion in Pennsylvania alone. Microbial Fe(II) oxidation is more efficient than abiotic Fe(II) oxidation at low pH (P. C. Singer and W. Stumm, Science 167:1121–1123, 1970, https://doi.org/10.1126/science.167.3921.1121 ). Therefore, AMD bioremediation could harness microbial Fe(II) oxidation to fuel more-cost-effective treatments. Advances will require a deeper understanding of the ecology of Fe(II)-oxidizing microbial communities and the factors that control their distribution and rates of Fe(II) oxidation. We investigated bacterial communities that inhabit an AMD site with rapid Fe(II) oxidation and found that they were dominated by two operational taxonomic units (OTUs) of Ferrovum myxofaciens, a taxon associated with high laboratory rates of iron oxidation. This research represents a step forward in identifying taxa that can be used to enhance cost-effective AMD bioremediation.

scholarly journals Microbial Community Diversity Dynamics in Acid Mine Drainage and Acid Mine Drainage-Polluted Soils: Implication on Mining Water Irrigation Agricultural Sustainability

2021 ◽  
Vol 5 ◽  
Author(s):  
Rabelani Munyai ◽  
Henry Joseph Oduor Ogola ◽  
David Mxolisi Modise
Keyword(s):  
Acid Mine Drainage ◽  
Plant Growth ◽  
Microbial Diversity ◽  
Agricultural Production ◽  
Mine Drainage ◽  
Agricultural Soils ◽  
Soil Health ◽  
Ecological Niches ◽  
Acid Mine ◽  
The Impact

Environmental degradation related to mining-generated acid mine drainage (AMD) is a major global concern, contaminating surface and groundwater sources, including agricultural land. In the last two decades, many developing countries are expanding agricultural productivity in mine-impacted soils to meet food demand for their rapidly growing population. Further, the practice of AMD water (treated or untreated) irrigated agriculture is on the increase, particularly in water-stressed nations around the world. For sustainable agricultural production systems, optimal microbial diversity, and functioning is critical for soil health and plant productivity. Thus, this review presents up-to-date knowledge on the microbial structure and functional dynamics of AMD habitats and AMD-impacted agricultural soils. The long-term effects of AMD water such as soil acidification, heavy metals (HM), iron and sulfate pollution, greatly reduces microbial biomass, richness, and diversity, impairing soil health plant growth and productivity, and impacts food safety negatively. Despite these drawbacks, AMD-impacted habitats are unique ecological niches for novel acidophilic, HM, and sulfate-adapted microbial phylotypes that might be beneficial to optimal plant growth and productivity and bioremediation of polluted agricultural soils. This review has also highlighted the impact active and passive treatment technologies on AMD microbial diversity, further extending the discussion on the interrelated microbial diversity, and beneficial functions such as metal bioremediation, acidity neutralization, symbiotic rhizomicrobiome assembly, and plant growth promotion, sulfates/iron reduction, and biogeochemical N and C recycling under AMD-impacted environment. The significance of sulfur-reducing bacteria (SRB), iron-oxidizing bacteria (FeOB), and plant growth promoting rhizobacteria (PGPRs) as key players in many passive and active systems dedicated to bioremediation and microbe-assisted phytoremediation is also elucidated and discussed. Finally, new perspectives on the need for future studies, integrating meta-omics and process engineering on AMD-impacted microbiomes, key to designing and optimizing of robust active and passive bioremediation of AMD-water before application to agricultural production is proposed.

Roles of Oligotrophic Acidophiles (Alicyclobacillus) in Chalcopyrite Bioleaching System: Shake Flask Evaluation

2015 ◽  
Vol 1130 ◽  
pp. 410-413
Author(s):  
Xing Yu Liu ◽  
Ming Jiang Zhang ◽  
Wen Yan Liu ◽  
Bo Wei Chen ◽  
Chun Yu Meng ◽  
...  
Keyword(s):  
Acid Mine Drainage ◽  
Bacterial Community ◽  
Mine Drainage ◽  
Community Change ◽  
Rrna Gene ◽  
Acid Mine ◽  
Copper Leaching ◽  
Culture Independent ◽  
Different Temperatures ◽  
Two Temperatures

An oligotrophic culture of acidophiles (Alicyclobacillus) isolated from Dexin acid mine drainage was evaluated for its synergistic effect in chalcopyrite bioleaching. Bioleaching of chalcopyrite with and without theAlicyclobacillusculture was investigated at different temperatures (33°C, 45 °C and 65 °C) and a culture-independent approach based on 16S rRNA gene clone library was used to analyze changes in the microbial community change during the bioleaching process. For 33oC leaching tests, only the bacterial community was analyzed, but for the other two temperatures, both the bacterial community and archaea communities were analyzed. Results showed that at high leaching temperature (65°C),Alicyclobacillusculture could increase copper leaching recovery from 57.83% to 60.7%. While at relative low temperature (45°C and 33°C), addingAlicyclobacillusculture inhibited copper bioleaching, copper leaching recovery decreased from 36.10% to 31.52% and from 34.02% to 21.97% respectively at 45°C and 33°C. Clone libraries analysis showed thatAlicyclobacillushelps the growth of genusSulfobacillusat 45 °C while inhibiting the growth of genusLeptospillumat both 33°C and 45 °C. Furthermore, when addingAlicyclobacillusgrowth ofFerroplasmawas limited andAcidoplasmawas facilitated at 45°C. At 60°C, addingAlicyclobacillusculture facilitated the growth of genusMetallosphaerawhile limiting the growth ofLeptospillumandFerroplasma. The results showed potential application ofAlicyclobacillusin high temperature chalcopyrite bioleaching and bioremediation of acid mine drainage.

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