In vitro and in vivo studies on devices of poly(l-co-d,l lactic acid)-co-TMC for bone repair

2018 ◽  
Vol 75 (10) ◽  
pp. 4515-4529 ◽  
Author(s):  
Adriana C. Motta ◽  
Vitor de Miranda Fedrizzi ◽  
Maria Lourdes Peri Barbo ◽  
Eliana A. R. Duek
Keyword(s):  
Lactic Acid ◽  
Bone Repair ◽  
In Vivo Studies

scholarly journals A biomimetically hierarchical polyetherketoneketone scaffold for osteoporotic bone repair

2020 ◽  
Vol 6 (50) ◽  
pp. eabc4704
Author(s):  
Bo Yuan ◽  
Linnan Wang ◽  
Rui Zhao ◽  
Xi Yang ◽  
Xiao Yang ◽  
...  
Keyword(s):  
Bone Repair ◽  
Osteoporotic Fractures ◽  
In Vivo Studies ◽  
Osteoporotic Bone ◽  
Worldwide Population ◽  
Repair Materials ◽  
Porous Architecture ◽  
Viable Approach

Osteoporotic fractures are prevalent in society, and their incidence appears to be increasing as the worldwide population ages. However, conventional bone repair materials hardly satisfy the requirements for the repair of pathological fractures. Here, we developed a biomimetic polyetherketoneketone scaffold with a functionalized strontium-doped nanohydroxyapatite coating for osteoporotic bone defect applications. The scaffold has a hierarchically porous architecture and mechanical strength similar to that of osteoporotic trabecular bone. In vitro and in vivo studies demonstrated that the scaffold could promote osteoporotic bone regeneration and delay adjacent bone loss via regulating both osteoblasts and osteoclasts. In addition, the correlations between multiple preimplantation and postimplantation parameters were evaluated to determine the potential predictors of in vivo performance of the material. The current work not only develops a promising candidate for osteoporotic bone repair but also provides a viable approach for designing other functional biomaterials and predicting their translational value.

Evaluation of Peanut Flour Fermented with Lactic Acid Bacteria as a Probiotic Food

2007 ◽  
Vol 13 (6) ◽  
pp. 469-475 ◽  
Author(s):  
N.-F. Wang ◽  
Y.-H. Shi ◽  
J. Sun ◽  
G.-W. Le
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Marked Change ◽  
Lactobacillus Delbrueckii ◽  
In Vivo Studies ◽  
Peanut Flour ◽  
Control Group ◽  
Probiotic Food

The aim of this study was to evaluate the probiotic value of peanut flour fermented with lactic acid bacteria in vitro and in vivo. Four strains including Lactobacillus delbrueckii LD09, Lactobacillus casei LC35, Lactobacillus acidophilus LA51, and Lactobacillus plantarum P9 were screened for their growth and survival in peanut flour. Among all the strains, L. plantarum P9 grew to the highest cell population (9.48 log cfu/g) in peanut flour after 72 h fermentation at 37°C. After 28 days storage at 4°C, no marked change in the viable count of this strain was observed. Peanut flour fermented with L. plantarum P9 could also increase the content of crude protein and the degree of protein hydrolysis. In an in vitro system, the addition of protein from the fermented peanut flour greatly enhanced the survival of L. plantarum P9 in simulated gastric and bile juices. In vivo studies, supplementation with the fermented peanut flour in the diet of mice increased significantly the number of lactobacilli in the fecal samples compared to the control group. At the same time, the number of enterobacteria decreased significantly. These results indicated that peanut flour fermented with L. plantarum P9 strain could be a novel type of probiotic food.

scholarly journals CHARACTERISTICS AND MECHANISM OF POTENTIAL PROBIOTICS WITH SPECIAL REFERENCE TO LACTIC ACID BACTERIA FROM TRADITIONAL FERMENTED FISH PRODUCTS: A REVIEW

2021 ◽  
Vol 9 (3) ◽  
pp. 263-275
Author(s):  
Soibam Ngasotter ◽  
◽  
David Waikhom ◽  
Sanjeev Sharma ◽  
Maibam Malemngamba Meitei ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Food Industry ◽  
In Vivo Studies ◽  
Probiotic Properties ◽  
Positive Health ◽  
Fish Products ◽  
Fermented Fish

Lactic acid bacteria (LAB) are widely used in the food industry due to their probiotic properties and fermentation activities. Traditional fermented fish products are dominated by a diverse variety of lactic acid bacteria with significant probiotic characteristics. Several in vitro and in vivo studies on lactic acid bacteria from fermented fish products have confirmed LAB strains to possess characteristics to be considered as probiotics that contribute to positive health benefits to the host and are generally regarded as safe (GRAS). This paper presents a review of the characteristics of the LAB strain that is considered as a probiotic. It also presents an overview of the probiotics mechanism of action and specifically highlights the LAB species with potential probiotic characteristics isolated from traditional fermented fish products.

In Vitro and In Vivo Evaluation of Bioactive Glass/PVA Porous Hybrids for Application in Bone Reconstruction

2008 ◽  
Vol 396-398 ◽  
pp. 671-674
Author(s):  
Viviane Gomide ◽  
Natália Ocarino ◽  
Rogéria Serakides ◽  
Maria de Fatima Leite ◽  
Marivalda Pereira
Keyword(s):  
Bioactive Glass ◽  
Bone Repair ◽  
Sol Gel ◽  
Bone Matrix ◽  
In Vivo Studies ◽  
Adult Rats ◽  
Osteoblast Culture ◽  
Hybrid Foams

Bioactive glass/polymer hybrids are promising materials for biomedical applications because they combine the bioactivity of these bioceramics with the flexibility of polymers. In previous work hybrid foams with 50% bioactive glass and 50% polyvinyl alcohol (PVA) were prepared by the sol-gel process for application as scaffold for bone tissue engineering. In this work the hybrid samples were tested in osteoblast culture to evaluate adhesion and proliferation. Samples were also implanted subcutaneously in the dorsal region of adult rats. The hybrid 50% PVA/bioactive glass foam was chosen as the best scaffold in the composition range studied and it is a promising material for bone repair, providing a good environment for the adhesion and proliferation of osteoblasts in vitro. Concerning the in vivo studies we can assure that the “foreing body” reaction was moderate and that the presence of osteoid indicated bone matrix formation.

scholarly journals Screening of Bacteriocinogenic Lactic Acid Bacteria and Their Characterization as Potential Probiotics

2020 ◽  
Vol 8 (3) ◽  
pp. 393 ◽  
Author(s):  
Ana Pinto ◽  
Joana Barbosa ◽  
Helena Albano ◽  
Joana Isidro ◽  
Paula Teixeira
Keyword(s):  
Lactic Acid ◽  
Gastrointestinal Tract ◽  
Lactic Acid Bacteria ◽  
Foodborne Pathogens ◽  
Food Products ◽  
In Vivo Studies ◽  
Rrna Gene ◽  
Gene Sequence Analysis

Probiotics are living microorganisms used as nutritional additives that confer health benefits on the host. Their use in food products is very attractive, especially if they could also inhibit important foodborne pathogens. In this study, antimicrobial activity against several foodborne pathogens was screened for 280 lactic acid bacteria (LAB) isolated from different food products and the probiotic characteristics of bacteriocinogenic isolates were evaluated. Seven out of 280 LAB isolates were selected due to their bacteriocinogenic properties and identified by 16S rRNA gene sequence analysis as Pediococcus pentosaceus (n = 6) and Lactobacillus plantarum (n = 1). Virulence factors and antibiotic resistances were not detected for any of the isolates. Except for L. plantarum R23, all the isolates were able to survive through the simulated gastrointestinal tract conditions. Only P. pentosaceus CFF4 was able to adhere to Caco-2 cells after the simulated gastrointestinal tract passage. In conclusion, even though in vivo studies should be performed, P. pentosaceus CFF4, which was also able to inhibit the growth of foodborne pathogens in vitro, seems to be a potential probiotic to be used in the food industry.

In Vitro and in Vivo Studies of Novel Poly(d,l-lactic acid), Superhydrophilic Carbon Nanotubes, and Nanohydroxyapatite Scaffolds for Bone Regeneration

2015 ◽  
Vol 7 (18) ◽  
pp. 9385-9398 ◽  
Author(s):  
Idalia A. W. B. Siqueira ◽  
Marcus Alexandre F. Corat ◽  
Bruno das Neves Cavalcanti ◽  
Wilson Alves Ribeiro Neto ◽  
Airton Abrahao Martin ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Lactic Acid ◽  
Bone Regeneration ◽  
In Vivo Studies

Electrospun Nanofibrous P(DLLA–CL) Balloons as Calcium Phosphate Cement Filled Containers for Bone Repair: in Vitro and in Vivo Studies

2015 ◽  
Vol 7 (33) ◽  
pp. 18540-18552 ◽  
Author(s):  
Xunwei Liu ◽  
Daixu Wei ◽  
Jian Zhong ◽  
Mengjia Ma ◽  
Juan Zhou ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Calcium Phosphate Cement ◽  
Bone Repair ◽  
In Vivo Studies

scholarly journals Synthesis of a bone like composite material derived from waste pearl oyster shells for potential bone tissue bioengineering applications

2017 ◽  
Vol 5 (6) ◽  
pp. 2454 ◽  
Author(s):  
Ravi Krishna Brundavanam ◽  
Derek Fawcett ◽  
Gérrard Eddy Jai Poinern
Keyword(s):  
Composite Materials ◽  
Bone Tissue ◽  
Bone Repair ◽  
Oyster Shell ◽  
Surface Characteristics ◽  
Pearl Oyster ◽  
In Vivo Studies ◽  
Pinctada Maxima

Background: Hydroxyapatite is generally considered a viable substitute for bone in a number of medical procedures such as bone repair, bone augmentation and coating metal implants. Unfortunately, hydroxyapatite has poor mechanical properties that make it unsuitable for many load bearing applications.Methods: In the present work various grades of finely crushed Pinctada maxima (pearl oyster shell) were combined with a nanometer scale hydroxyapatite powder to form novel composite materials. A comparative study was made between the various powder based composites synthesized. The crystalline structure and morphology of the various powder based composites were investigated using X-ray diffraction and field emission scanning electron microscopy. The composite materials were also evaluated and characterized.Results: Manufactured hydroxyapatite powders were composed of crystalline spherical/granular particles with a mean size of 30 nm. Also produced were hydroxyapatite and finely crushed calcium carbonate from Pinctada maxima (pearl oyster shell) powder mixtures. Hydroxyapatite coatings produced on Pinctada maxima nacre substrates were investigated and their surface characteristics reported.Conclusions: Pinctada maxima nacre pre-treated with sodium hypo chlorate before hydroxyapatite deposition produced a superior coating and could be used for bone tissue engineering. But further in vitro and in vivo studies are needed to validate the biocompatibility and long term stability of this composite coating.

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