scholarly journals A biomimetically hierarchical polyetherketoneketone scaffold for osteoporotic bone repair

2020 ◽  
Vol 6 (50) ◽  
pp. eabc4704
Author(s):  
Bo Yuan ◽  
Linnan Wang ◽  
Rui Zhao ◽  
Xi Yang ◽  
Xiao Yang ◽  
...  
Keyword(s):  
Bone Repair ◽  
Osteoporotic Fractures ◽  
In Vivo Studies ◽  
Osteoporotic Bone ◽  
Worldwide Population ◽  
Repair Materials ◽  
Porous Architecture ◽  
Viable Approach

Osteoporotic fractures are prevalent in society, and their incidence appears to be increasing as the worldwide population ages. However, conventional bone repair materials hardly satisfy the requirements for the repair of pathological fractures. Here, we developed a biomimetic polyetherketoneketone scaffold with a functionalized strontium-doped nanohydroxyapatite coating for osteoporotic bone defect applications. The scaffold has a hierarchically porous architecture and mechanical strength similar to that of osteoporotic trabecular bone. In vitro and in vivo studies demonstrated that the scaffold could promote osteoporotic bone regeneration and delay adjacent bone loss via regulating both osteoblasts and osteoclasts. In addition, the correlations between multiple preimplantation and postimplantation parameters were evaluated to determine the potential predictors of in vivo performance of the material. The current work not only develops a promising candidate for osteoporotic bone repair but also provides a viable approach for designing other functional biomaterials and predicting their translational value.

In vitro and in vivo studies on devices of poly(l-co-d,l lactic acid)-co-TMC for bone repair

2018 ◽  
Vol 75 (10) ◽  
pp. 4515-4529 ◽  
Author(s):  
Adriana C. Motta ◽  
Vitor de Miranda Fedrizzi ◽  
Maria Lourdes Peri Barbo ◽  
Eliana A. R. Duek
Keyword(s):  
Lactic Acid ◽  
Bone Repair ◽  
In Vivo Studies

scholarly journals Fabrication of Injectable, Porous Hyaluronic Acid Hydrogel Based on an In-Situ Bubble-Forming Hydrogel Entrapment Process

Polymers ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1138
Author(s):  
Lixuan Wang ◽  
Shiyan Dong ◽  
Yutong Liu ◽  
Yifan Ma ◽  
Jingjing Zhang ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Regenerative Medicine ◽  
In Vivo Studies ◽  
Injectable Hydrogels ◽  
Cell Behaviors ◽  
Porous Architecture ◽  
In Situ Hydrogel

Injectable hydrogels have been widely applied in the field of regenerative medicine. However, current techniques for injectable hydrogels are facing a challenge when trying to generate a biomimetic, porous architecture that is well-acknowledged to facilitate cell behaviors. In this study, an injectable, interconnected, porous hyaluronic acid (HA) hydrogel based on an in-situ bubble self-generation and entrapment process was developed. Through an amide reaction between HA and cystamine dihydrochloride activated by EDC/NHS, CO2 bubbles were generated and were subsequently entrapped inside the substrate due to a rapid gelation-induced retention effect. HA hydrogels with different molecular weights and concentrations were prepared and the effects of the hydrogel precursor solution’s concentration and viscosity on the properties of hydrogels were investigated. The results showed that HA10-10 (10 wt.%, MW 100,000 Da) and HA20-2.5 (2.5 wt.%, MW 200,000 Da) exhibited desirable gelation and obvious porous structure. Moreover, HA10-10 represented a high elastic modulus (32 kPa). According to the further in vitro and in vivo studies, all the hydrogels prepared in this study show favorable biocompatibility for desirable cell behaviors and mild host response. Overall, such an in-situ hydrogel with a self-forming bubble and entrapment strategy is believed to provide a robust and versatile platform to engineer injectable hydrogels for a variety of applications in tissue engineering, regenerative medicine, and personalized therapeutics.

scholarly journals MSC-derived small extracellular vesicles overexpressing miR-20a promoted the osteointegration of porous titanium alloy by enhancing osteogenesis via targeting BAMBI

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Wei Liu ◽  
Jinghuan Huang ◽  
Feng Chen ◽  
Dong Xie ◽  
Longqing Wang ◽  
...  
Keyword(s):  
Titanium Alloy ◽  
Extracellular Vesicles ◽  
Porous Titanium ◽  
Fluorescent Labeling ◽  
In Vivo Studies ◽  
Osteoporotic Bone ◽  
Alizarin Red ◽  
Alkaline Phosphatase Staining

Abstract Background Patients with osteoporosis have a high risk of implant loosening due to poor osteointegration, possibly leading to implant failure, implant revision, and refracture. RNA interference therapy is an emerging epigenetic treatment, and we found that miR-20a could enhance osteogenesis. Moreover, small extracellular vesicles (sEVs) derived from bone marrow mesenchymal stem cells (hBM-MSCs) were utilized as nanoscale carriers for the protection and transportation of miR-20a (sEV-20a). In this study, we intended to determine whether sEVs overexpressing miR-20a could exert a superior effect on osteoporotic bone defects and the underlying mechanism. Methods For evaluating the effect of sEV-20a on osteogenesis, in vitro and in vivo studies were performed. In vitro, we first showed that miR-20a was upregulated in the osteogenic process and overexpressed sEVs with miR-20a by the transfection method. Then, the proliferation, migration, and osteogenic differentiation abilities of hBM-MSCs treated with sEV-20a were detected by CCK-8 assays, alkaline phosphatase staining and alizarin red staining, qRT-PCR, and western blot. In vivo, we established an osteoporotic bone defect model and evaluated the effect of sEV-20a on bone formation by micro-CT, sequential fluorescent labeling, and histological analysis. To further explore the mechanism, we applied a bioinformatics method to identify the potential target of miR-20a. Results In vitro, sEV-20a was successfully established and proved to promote the migration and osteogenesis of hBM-MSCs. In vivo, sEV-20a promoted osteointegration in an osteoporotic rat model. To further elucidate the related mechanism, we proved that miR-20a could enhance osteogenesis by targeting BAMBI. Conclusions Collectively, the in vitro and in vivo results confirmed that MSC-derived sEV-20a therapy effectively promoted osteoporotic porous titanium alloy osteointegration via pro-osteogenic effects by targeting BAMBI.

In Vitro and In Vivo Evaluation of Bioactive Glass/PVA Porous Hybrids for Application in Bone Reconstruction

2008 ◽  
Vol 396-398 ◽  
pp. 671-674
Author(s):  
Viviane Gomide ◽  
Natália Ocarino ◽  
Rogéria Serakides ◽  
Maria de Fatima Leite ◽  
Marivalda Pereira
Keyword(s):  
Bioactive Glass ◽  
Bone Repair ◽  
Sol Gel ◽  
Bone Matrix ◽  
In Vivo Studies ◽  
Adult Rats ◽  
Osteoblast Culture ◽  
Hybrid Foams

Bioactive glass/polymer hybrids are promising materials for biomedical applications because they combine the bioactivity of these bioceramics with the flexibility of polymers. In previous work hybrid foams with 50% bioactive glass and 50% polyvinyl alcohol (PVA) were prepared by the sol-gel process for application as scaffold for bone tissue engineering. In this work the hybrid samples were tested in osteoblast culture to evaluate adhesion and proliferation. Samples were also implanted subcutaneously in the dorsal region of adult rats. The hybrid 50% PVA/bioactive glass foam was chosen as the best scaffold in the composition range studied and it is a promising material for bone repair, providing a good environment for the adhesion and proliferation of osteoblasts in vitro. Concerning the in vivo studies we can assure that the “foreing body” reaction was moderate and that the presence of osteoid indicated bone matrix formation.

Multi-stage controllable degradation of strontium-doped calcium sulfate hemihydrate-Tricalcium phosphate microsphere composite as a substitute for osteoporotic bone defect repairing: Degradation behavior and bone response

2021 ◽  
Author(s):  
Qiuju Miao ◽  
Nan Jiang ◽  
Qinmeng Yang ◽  
Ismail mohamed Hussein ◽  
Zhen Luo ◽  
...  
Keyword(s):  
Tricalcium Phosphate ◽  
Calcium Sulfate ◽  
Bone Repair ◽  
Degradation Rate ◽  
Bone Defects ◽  
Osteoporotic Bone ◽  
Repair Materials ◽  
Bone Repair Materials ◽  
In Vivo Degradation

Abstract Various requirements for the repair of complex bone defects have motivated to development of scaffolds with adjustable degradation rates and biological functions. Tricalcium phosphate and calcium sulfate are the most commonly used bone repair materials in the clinic, how to better combine tricalcium phosphate and calcium sulfate and play their greatest advantages in the repair of osteoporotic bone defect is the focus of our research. In this study, a series of scaffolds with multistage-controlled degradation properties composed of strontium-doped calcium sulfate (SrCSH) and strontium-doped tricalcium phosphate microspheres (Sr-TCP) scaffolds were prepared, and their osteogenic activity, in vivo degradation and bone regeneration ability in tibia of osteoporotic rats were evaluated. In vitro studies revealed that different components of SrCSH/Sr-TCP scaffolds significantly promoted the proliferation and differentiation of MC3T3-E1 cells, which showed a good osteogenic induction activity. In vivo degradation results showed that the degradation time of composite scaffolds could be controlled in a large range (6-12 months) by controlling the porosity and phase composition of Sr-TCP microspheres. The results of osteoporotic femoral defect repair showed that when the degradation rate of scaffold matched with the growth rate of new bone, the parameters such as BMD, BV/TV, Tb.Th, angiogenesis marker CD31 and new bone formation marker OCN expression were higher, which promoted the rapid repair of osteoporotic bone defects. On the contrary, the slow degradation rate of scaffolds hindered the growth of new bone to a certain extent. This study elucidates the importance of the degradation rate of scaffolds for the repair of osteoporotic bone defects, and the design considerations can be extended to other bone repair materials, which is expected to provide new ideas for the development of tissue engineering materials in the future.

Electrospun Nanofibrous P(DLLA–CL) Balloons as Calcium Phosphate Cement Filled Containers for Bone Repair: in Vitro and in Vivo Studies

2015 ◽  
Vol 7 (33) ◽  
pp. 18540-18552 ◽  
Author(s):  
Xunwei Liu ◽  
Daixu Wei ◽  
Jian Zhong ◽  
Mengjia Ma ◽  
Juan Zhou ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Calcium Phosphate Cement ◽  
Bone Repair ◽  
In Vivo Studies

scholarly journals Synthesis of a bone like composite material derived from waste pearl oyster shells for potential bone tissue bioengineering applications

2017 ◽  
Vol 5 (6) ◽  
pp. 2454 ◽  
Author(s):  
Ravi Krishna Brundavanam ◽  
Derek Fawcett ◽  
Gérrard Eddy Jai Poinern
Keyword(s):  
Composite Materials ◽  
Bone Tissue ◽  
Bone Repair ◽  
Oyster Shell ◽  
Surface Characteristics ◽  
Pearl Oyster ◽  
In Vivo Studies ◽  
Pinctada Maxima

Background: Hydroxyapatite is generally considered a viable substitute for bone in a number of medical procedures such as bone repair, bone augmentation and coating metal implants. Unfortunately, hydroxyapatite has poor mechanical properties that make it unsuitable for many load bearing applications.Methods: In the present work various grades of finely crushed Pinctada maxima (pearl oyster shell) were combined with a nanometer scale hydroxyapatite powder to form novel composite materials. A comparative study was made between the various powder based composites synthesized. The crystalline structure and morphology of the various powder based composites were investigated using X-ray diffraction and field emission scanning electron microscopy. The composite materials were also evaluated and characterized.Results: Manufactured hydroxyapatite powders were composed of crystalline spherical/granular particles with a mean size of 30 nm. Also produced were hydroxyapatite and finely crushed calcium carbonate from Pinctada maxima (pearl oyster shell) powder mixtures. Hydroxyapatite coatings produced on Pinctada maxima nacre substrates were investigated and their surface characteristics reported.Conclusions: Pinctada maxima nacre pre-treated with sodium hypo chlorate before hydroxyapatite deposition produced a superior coating and could be used for bone tissue engineering. But further in vitro and in vivo studies are needed to validate the biocompatibility and long term stability of this composite coating.

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