Synchronisation of glycolytic activity in yeast cells

2021 ◽  
Author(s):  
Marcus J. B. Hauser
Keyword(s):  
Yeast Cells ◽  
Glycolytic Activity

Hyperpolarized [U-2H, U-13C]Glucose reports on glycolytic and pentose phosphate pathway activity in EL4 tumors and glycolytic activity in yeast cells

2014 ◽  
Vol 74 (6) ◽  
pp. 1543-1547 ◽  
Author(s):  
Kerstin N. Timm ◽  
Johannes Hartl ◽  
Markus A. Keller ◽  
De-En Hu ◽  
Mikko I. Kettunen ◽  
...  
Keyword(s):  
Pentose Phosphate Pathway ◽  
Pentose Phosphate ◽  
Yeast Cells ◽  
Pathway Activity ◽  
Glycolytic Activity

scholarly journals Novel Proteomic changes in Yeast Mitochondria provide insights into mitochondrial functioning upon over-expression of human p53

2019 ◽  
Author(s):  
Archana Kumari Redhu ◽  
Jayadeva Paike Bhat
Keyword(s):  
Carbon Source ◽  
Warburg Effect ◽  
Pentose Phosphate ◽  
Yeast Cells ◽  
Yeast Mitochondria ◽  
Over Expression ◽  
Cycle Arrest ◽  
Glycolytic Activity ◽  
Sucrose Fermentation ◽  
Induced Apoptosis

AbstractCancer cells display enhanced glycolytic activity and impaired oxidative phosphorylation even in the presence of adequate oxygen (Warburg effect). Mitochondrial physiology is a promising hit target for anti-cancer therapy because of its key role in Warburg effect and activating apoptosis in mammalian as well as yeast cells. Over-expression of human p53 in S.cerevisiae leads to cell cycle arrest and apotosis. In the present work we show that how S.cerevisiae escapes from p53 induced apoptosis in fermentable carbon source, whereas in case of non-fermentable carbon source this phenomenon is not observed. To shed the light on this aspect we performed a quantitative proteomic analysis of yeast mitochondria isolated from the cells grown on sucrose (fermentation) and glycerol (respiration) with and without p53 over-expression. Through this approach, we identified a total dataset of 1120 proteins with 1% FDR, of which 239(133+106) proteins are differentially experssed in both conditions. Interestingly, we observed that after over-expression of p53 in sucrose grown yeast cells, a complete set of pentose phosphate pathway (PPP) enzymes is up-regulated in the mitochondria that leads to enhanced mitochondrial NADPH production and ROS quenching. Increased association of a hexose transporter (HXT6) and a hexokinase (HXK2) with the mitochondria of fermenting yeast cells upon over-expression of p53, may direct glucose towards PPP inside the mitochondria. In conclusion, our results provide the evidence that up-regulated PPP inside the mitochondria is a key to evade apoptosis by S.cerevisiae upon p53 over-expression.

Selective enhancement of glycolytic activity by incubation of yeast cells in sugars and polyols

1968 ◽  
Vol 14 (11) ◽  
pp. 1245-1252 ◽  
Author(s):  
Edward Spoerl ◽  
R. J. Doyle
Keyword(s):  
Nicotinamide Adenine Dinucleotide ◽  
High Rate ◽  
Yeast Cells ◽  
Inorganic Salts ◽  
Cell Extracts ◽  
Glycolytic Activity ◽  
Lag Period ◽  
High Concentrations ◽  
Selective Enhancement ◽  
Purines And Pyrimidines

Incubation of yeast cells for 21 hours in solutions of certain sugars and polyols produces cells capable of forming CO2 from glucose at a high rate and without an initial lag as compared to cells starved by incubation in water. Other sugars and other compounds, including amino acids, purines and pyrimidines, and organic and inorganic salts, were ineffective or reduced CO2 production. Readily metabolized sugars were effective at concentrations of 0.001 M, poorly metabolized sugars required higher concentration, and non-metabolized sugars were ineffective. However, mannitol and sorbitol, non-metabolized polyols structurally related to utilized sugars, were effective. Alcohols and polyols added to the cells after incubation in water overcame the lag period. Sugars produced a persistent change, acted faster than polyols, and were more effective in the presence of non-effective compounds. Readily utilized sugars at high concentrations reduced cell viability and the decarboxylase- and CO2-producing capacities of cell extracts. The nicotinamide adenine dinucleotide contents of extracts did not indicate deficiencies, and differences in adenosine triphosphate contents appeared not to be significant. The data may be interpreted as evidence for an effect by the sugars and polyols primarily upon intracellular membranes.

Heterogeneity of Size and Distribution of Intramembrane Particles in the Plasma Membrane of Yeast

1977 ◽  
Vol 35 ◽  
pp. 596-597
Author(s):  
E. Keyhani
Keyword(s):  
Plasma Membrane ◽  
Candida Utilis ◽  
Synthetic Medium ◽  
Freeze Fracture ◽  
Translational Diffusion ◽  
Yeast Cells ◽  
Distilled Water ◽  
Intramembrane Particles ◽  
The Matrix ◽  
Water Cell

The matrix of biological membranes consists of a lipid bilayer into which proteins or protein aggregates are intercalated. Freeze-fracture techni- ques permit these proteins, perhaps in association with lipids, to be visualized in the hydrophobic regions of the membrane. Thus, numerous intramembrane particles (IMP) have been found on the fracture faces of membranes from a wide variety of cells (1-3). A recognized property of IMP is their tendency to form aggregates in response to changes in experi- mental conditions (4,5), perhaps as a result of translational diffusion through the viscous plane of the membrane. The purpose of this communica- tion is to describe the distribution and size of IMP in the plasma membrane of yeast (Candida utilis).Yeast cells (ATCC 8205) were grown in synthetic medium (6), and then harvested after 16 hours of culture, and washed twice in distilled water. Cell pellets were suspended in growth medium supplemented with 30% glycerol and incubated for 30 minutes at 0°C, centrifuged, and prepared for freeze-fracture, as described earlier (2,3).

Ultrathin frozen sections of yeast without aldehyde prefixation

1978 ◽  
Vol 36 (2) ◽  
pp. 58-59
Author(s):  
K. J. Böhm ◽  
a. E. Unger
Keyword(s):  
Aqueous Solutions ◽  
Biological Material ◽  
Yeast Cells ◽  
Frozen Sections ◽  
Good Preservation ◽  
Ultrathin Frozen Sections

During the last years it was shown that also by means of cryo-ultra-microtomy a good preservation of substructural details of biological material was possible. However the specimen generally was prefixed in these cases with aldehydes.Preparing ultrathin frozen sections of chemically non-prefixed material commonly was linked up to considerable technical and manual expense and the results were not always satisfying. Furthermore, it seems to be impossible to carry out cytochemical investigations by means of treating sections of unfixed biological material with aqueous solutions.We therefore tried to overcome these difficulties by preparing yeast cells (S. cerevisiae) in the following manner:

Deformation of Yeast Plasma Membrane by Ethidium Bromide

1988 ◽  
Vol 46 ◽  
pp. 254-255
Author(s):  
E. Keyhani
Keyword(s):  
Plasma Membrane ◽  
Thin Section ◽  
Ethidium Bromide ◽  
Freeze Fracture ◽  
Mutagenic Effect ◽  
Yeast Cells ◽  
Hydrophobic Region ◽  
Thin Section Electron Microscopy ◽  
Section Electron ◽  
Deep Pits

The mutagenic effect of ethidium bromide on the mitochondrial DNA is well established. Using thin section electron microscopy, it was shown that when yeast cells were grown in the presence of ethidium bromide, besides alterations in the mitochondria, the plasma membrane also showed alterations consisting of 75 to 110 nm-deep pits. Furthermore, ethidium bromide induced an increase in the length and number of endoplasmic reticulum and in the number of intracytoplasmic vesicles.Freeze-fracture, by splitting the hydrophobic region of the membrane, allows the visualization of the surface view of the membrane, and consequently, any alteration induced by ethidium bromide on the membrane can be better examined by this method than by the thin section method.Yeast cells, Candida utilis. were grown in the presence of 35 μM ethidium bromide. Cells were harvested and freeze-fractured according to the procedure previously described.

Three-Dimensional Immunoelectron Microscopy of Yeast Cells by a New High-Resolution Replica Method

1985 ◽  
Vol 43 ◽  
pp. 562-563
Author(s):  
Hirano T. ◽  
M. Yamaguchi ◽  
M. Hayashi ◽  
Y. Sekiguchi ◽  
A. Tanaka
Keyword(s):  
High Resolution ◽  
Plasma Polymerization ◽  
Three Dimensional ◽  
Freeze Fracture ◽  
Replica Method ◽  
Yeast Cells ◽  
Dynamic Aspect ◽  
Replica Technique ◽  
Gaseous Hydrocarbon ◽  
Transmission Electron

A plasma polymerization film replica method is a new high resolution replica technique devised by Tanaka et al. in 1978. It has been developed for investigation of the three dimensional ultrastructure in biological or nonbiological specimens with the transmission electron microscope. This method is based on direct observation of the single-stage replica film, which was obtained by directly coating on the specimen surface. A plasma polymerization film was deposited by gaseous hydrocarbon monomer in a glow discharge.The present study further developed the freeze fracture method by means of a plasma polymerization film produces a three dimensional replica of chemically untreated cells and provides a clear evidence of fine structure of the yeast plasma membrane, especially the dynamic aspect of the structure of invagination (Figure 1).

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