Expression of sarcotoxin IA gene via a root-specific tob promoter enhanced host resistance against parasitic weeds in tomato plants

2005 ◽  
Vol 25 (4) ◽  
pp. 297-303 ◽  
Author(s):  
Aly Radi ◽  
Plakhin Dina ◽  
Achdari Guy
Keyword(s):  
Host Resistance ◽  
Tomato Plants ◽  
Parasitic Weeds ◽  
Sarcotoxin Ia

scholarly journals Induced Expression of Sarcotoxin IA Enhanced Host Resistance Against Both Bacterial and Fungal Pathogens in Transgenic Tobacco

2000 ◽  
Vol 13 (8) ◽  
pp. 860-868 ◽  
Author(s):  
Ichiro Mitsuhara ◽  
Hiroki Matsufuru ◽  
Masahiro Ohshima ◽  
Hisatoshi Kaku ◽  
Yuki Nakajima ◽  
...  
Keyword(s):  
Fungal Infection ◽  
Transgenic Tobacco ◽  
Pseudomonas Syringae ◽  
Host Resistance ◽  
Fungal Pathogens ◽  
Erwinia Carotovora ◽  
Induced Expression ◽  
Sarcotoxin Ia ◽  
Resistance To Infection

We demonstrate here that induced expression of sarcotoxin IA, a bactericidal peptide from Sarcophaga peregrina, enhanced the resistance of transgenic tobacco plants to both bacterial and fungal pathogens. The peptide was produced with a modified PR1a promoter, which is further activated by salicylic acid treatment and necrotic lesion formation by pathogen infection. Host resistance to infection of bacteria Erwinia carotovora subsp. carotovora and Pseudomonas syringae pv. tabaci was shown to be dependent on the amounts of sarcotoxin IA expressed. Since we found antifungal activity of the peptide in vitro, transgenic seedlings were also inoculated with fungal pathogens Rhizoctonia solani and Pythium aphanidermatum. Transgenic plants expressing higher levels of sarcotoxin were able to withstand fungal infection and remained healthy even after 4 weeks, while control plants were dead by fungal infection after 2 weeks.

Engineering host resistance against parasitic weeds with RNA interference

2009 ◽  
Vol 65 (5) ◽  
pp. 460-466 ◽  
Author(s):  
John I Yoder ◽  
Pradeepa Gunathilake ◽  
Biao Wu ◽  
Natalya Tomilova ◽  
Alexey A Tomilov
Keyword(s):  
Rna Interference ◽  
Host Resistance ◽  
Parasitic Weeds

scholarly journals Direct conversion of carlactonoic acid to orobanchol by cytochrome P450 CYP722C in strigolactone biosynthesis

2019 ◽  
Vol 5 (12) ◽  
pp. eaax9067 ◽  
Author(s):  
Takatoshi Wakabayashi ◽  
Misaki Hamana ◽  
Ayami Mori ◽  
Ryota Akiyama ◽  
Kotomi Ueno ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Direct Conversion ◽  
Ring Closure ◽  
Wild Type ◽  
Tomato Plants ◽  
Parasitic Weeds ◽  
Key Enzyme ◽  
Resistance To Infection

Strigolactones (SLs) are carotenoid-derived phytohormones and rhizosphere signaling molecules for arbuscular mycorrhizal fungi and root parasitic weeds. Why and how plants produce diverse SLs are unknown. Here, cytochrome P450 CYP722C is identified as a key enzyme that catalyzes the reaction of BC-ring closure leading to orobanchol, the most prevalent canonical SL. The direct conversion of carlactonoic acid to orobanchol without passing through 4-deoxyorobanchol is catalyzed by the recombinant enzyme. By knocking out the gene in tomato plants, orobanchol was undetectable in the root exudates, whereas the architecture of the knockout and wild-type plants was comparable. These findings add to our understanding of the function of the diverse SLs in plants and suggest the potential of these compounds to generate crops with greater resistance to infection by noxious root parasitic weeds.

scholarly journals Rhizobacteria induces resistance against Fusarium wilt of tomato by increasing the activity of defense enzymes

Bragantia ◽  
2014 ◽  
Vol 73 (3) ◽  
pp. 274-283 ◽  
Author(s):  
Hélvio Gledson Maciel Ferraz ◽  
Renata Sousa Resende ◽  
Patrícia Ricardino Silveira ◽  
Camila Cristina Lage Andrade ◽  
Elisângela Aparecida Milagres ◽  
...  
Keyword(s):  
Jasmonic Acid ◽  
Fusarium Wilt ◽  
Host Resistance ◽  
Plasma Membranes ◽  
Integrated Management ◽  
Positive Control ◽  
Defense Enzymes ◽  
Tomato Plants ◽  
Tomato Yield ◽  
Progress Curve

Fusarium wilt, caused by Fusarium oxysporum f.sp. lycopersici (Fol), is one of the most important diseases that affect tomato yield worldwide. This study investigated the potential of three antagonists, Streptomyces setonii (UFV 618), Bacillus cereus (UFV 592) and Serratia marcescens (UFV 252), and as positive control the hormone jasmonic acid (JA), to reduce Fusarium wilt symptoms and to potentiate the defense enzymes in the stem tissues of tomato plants infected by Fol. The seeds were microbiolized with each antagonist, and the soil was also drenched with them. The plants were sprayed with JA 48 h before Fol inoculation. The area under the Fusarium wilt index progress curve was reduced by 54, 48, 47 and 45% for the UFV 618, JA, UFV 592 and UFV 252 treatments, respectively. The three antagonists, and even the JA spray, efficiently reduced the Fusarium wilt symptoms on the tomato plant stems, which can be explained by the lower malondialdehyde concentration (an indication of oxidative damage to lipids in the plasma membranes) and the greater activities of peroxidases, polyphenoloxidases, glucanases, chitinases, phenylalanine ammonia-lyases and lipoxygenases, which are commonly involved in host resistance against fungal diseases. These results present a novel alternative that can be used in the integrated management of Fusarium wilt on tomatoes.

Tobacco Mosaic Virus-Infected Tissue

1985 ◽  
Vol 43 ◽  
pp. 510-511
Author(s):  
Egbert W. Henry
Keyword(s):  
Nicotiana Tabacum ◽  
Tobacco Mosaic Virus ◽  
Chlorogenic Acid ◽  
Mosaic Virus ◽  
Host Resistance ◽  
Oxidative Metabolism ◽  
Leaf Tissue ◽  
Vein Clearing ◽  
Basal Septum ◽  
Concentration Levels

Tobacco mosaic virus (TMV) infection has been studied in several investigations of Nicotiana tabacum leaf tissue. Earlier studies have suggested that TMV infection does not have precise infective selectivity vs. specific types of tissues. Also, such tissue conditions as vein banding, vein clearing, liquification and suberization may result from causes other than direct TMV infection. At the present time, it is thought that the plasmodesmata, ectodesmata and perhaps the plasmodesmata of the basal septum may represent the actual or more precise sites of TMV infection.TMV infection has been implicated in elevated levels of oxidative metabolism; also, TMV infection may have a major role in host resistance vs. concentration levels of phenolic-type enzymes. Therefore, enzymes such as polyphenol oxidase, peroxidase and phenylalamine ammonia-lyase may show an increase in activity in response to TMV infection. It has been reported that TMV infection may cause a decrease in o-dihydric phenols (chlorogenic acid) in some tissues.

Hypersensitive lignification response as the mechanism of non-host resistance of wheat against oat crown rust

1990 ◽  
Vol 78 (4) ◽  
pp. 609-615 ◽  
Author(s):  
Bruno M. Moerschbacher ◽  
Ulrike Noll ◽  
Carlos A. Ocampo ◽  
Berenike E. Flott ◽  
Uwe Gotthardt ◽  
...  
Keyword(s):  
Host Resistance ◽  
Crown Rust ◽  
Oat Crown Rust

Bed behavioral assessment of tomato varieties in Cote d Ivoire

2015 ◽  
Vol 5 (1) ◽  
pp. 513-522
Author(s):  
Jean-Claude N'ZI ◽  
Lassina FONDIO ◽  
Mako Francois De Paul N’GBESSO ◽  
Andé Hortense DJIDJI ◽  
Christophe KOUAME
Keyword(s):  
Behavioral Assessment ◽  
Development Stage ◽  
Research Centre ◽  
Vegetative Development ◽  
Potential Yield ◽  
Tomato Plants ◽  
Bacterial Diseases ◽  
Damage Rate ◽  
Fruit Damage ◽  
Tomato Breeding

Thirty accessions of tomato including twenty eight introduced accessions from The World Vegetable Center-AVRDC and as controls, two commercial varieties Mongal and Calinago, were assessed for agronomic performances at the Experimentation and Production Station of Angud dou of the National Agronomic Research Centre (CNRA) located in the South of Cote d Ivoire. The trial was arranged in a randomized block with three replications. The following parameters were determined at vegetative development stage: plant height at flowering stage, susceptibility of accessions to diseases, day to 50% flowering and day of first harvest, production duration, fruit length, fruit diameter, total number of fruits, number of fruits per plant, potential yield, net yield and fruit damage rate. Results showed that the commercial variety Mongal, with a potential yield of 15.9 and a net yield of 13.1 t ha-1, was the most productive. All the introduced accessions from AVRDC recorded the lowest potential yields from 2.2 to 9.7 t ha-1, and net yields from 1.7 to 8.6 t ha-1. In addition, accessions WVCT8, FMTT847 and WVCT13 were severely infested by bacterial wilt. The reduction of the net yield of tomato accessions resulted in the high fruit damage rates. For the future tomato breeding work, it would be appropriate to introduce into the trials bacterial diseases tolerant varieties. Moreover, some studies could be undertaken to determine the nature of the bacteria involved in the plant wilting and to find out the causal agent of the tomato plants burning at the fructification stage reducing the harvest duration.

Evaluation of tomato hybrids with different level of ripe rate under hydroponic conditions (fitopyramide)

2020 ◽  
Author(s):  
M.N. AL-Rukabi ◽  
◽  
V.I. Leunov
Keyword(s):  
Mineral Nutrient ◽  
Tomato Plants ◽  
Agricultural Method ◽  
Seedling Germination ◽  
Greenhouse Tomatoes ◽  
Hydroponic Conditions ◽  
Nutrient Solutions ◽  
Variety Testing

Greenhouse tomatoes are divided into early, medium and late-maturing. The days from seedling germination to the first harvest are taken into account. Tomato has a huge potential for heterosis in terms of precocity, overall yield, signs of resistance and uniformity. The preferred agricultural method is hydroponics, which allows you to grow plants without using soil, only using mineral nutrient solutions in water. The cultivation of tomato plants on the " Fitopyramida " will allow to sell their products in the periods with the highest realized prices. An experiment on variety testing of 11 tomato hybrids of different product groups that differ in precocity allowed us to select the most adapted to the conditions of the " Fitopyramida " technology, including the indeterminate beef Ruddy ball F1, cherry hybrids Elf F1 and orange-fruited cherry Magic harp F1. the determinant hybrid Captain F1 showed Good results.

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