Plant regeneration via somatic embryogenesis, and transient gene expression in sweet potato protoplasts

1998 ◽  
Vol 17 (9) ◽  
pp. 665-669 ◽  
Author(s):  
S. K. Dhir ◽  
J. Oglesby ◽  
A. S. Bhagsari
Keyword(s):  
Gene Expression ◽  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Sweet Potato ◽  
Transient Gene Expression

Somatic embryogenesis and plant regeneration in tissue cultures of sweet potato (Ipomea batatas Poir.)

1984 ◽  
Vol 3 (3) ◽  
pp. 112-115 ◽  
Author(s):  
Jang R. Liu ◽  
Daniel J. Cantliffe
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Sweet Potato ◽  
Tissue Cultures ◽  
Ipomea Batatas

scholarly journals GUS Gene expression and plant regeneration via somatic embryogenesis in cucumber (Cucumis sativus L.)

2008 ◽  
Vol 35 (4) ◽  
pp. 275-280 ◽  
Author(s):  
Hyun-A Kim ◽  
Boo-Youn Lee ◽  
Jin-Jung Jeon ◽  
Dong-Woog Choi ◽  
Pil-Son Choi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Cucumis Sativus ◽  
Gus Gene ◽  
Cucumis Sativus L ◽  
Gus Gene Expression

Isozyme modifications and plant regeneration through somatic embryogenesis in sweet potato (Ipomoea batatas (L.) Lam.)

1994 ◽  
Vol 13 (8) ◽  
Author(s):  
J.M. Cavalcante Alves ◽  
D. Sihachakr ◽  
M. Allot ◽  
S. Tizroutine ◽  
I. Mussio ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Sweet Potato ◽  
Ipomoea Batatas

Optimization of transient gene expression in pollen of Norway spruce (Picea abies) by particle acceleration

1994 ◽  
Vol 92 (3) ◽  
pp. 412-416 ◽  
Author(s):  
Inger Martinussen ◽  
Olavi Junttila ◽  
David Twell
Keyword(s):  
Gene Expression ◽  
Particle Acceleration ◽  
Picea Abies ◽  
Norway Spruce ◽  
Transient Gene Expression

Somatic Embryogenesis and Plant Regeneration from Suspension Cultures of Red Fescue

Crop Science ◽  
1989 ◽  
Vol 29 (3) ◽  
pp. 815-817 ◽  
Author(s):  
O. M. F. Zaghmout ◽  
W. A. Torello
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Suspension Cultures ◽  
Red Fescue

scholarly journals Harnessing a Transient Gene Expression System in Nicotiana benthamiana to Explore Plant Agrochemical Transporters

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 524
Author(s):  
Bingqi Wu ◽  
Zhiting Chen ◽  
Xiaohui Xu ◽  
Ronghua Chen ◽  
Siwei Wang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transient Expression ◽  
Nicotiana Benthamiana ◽  
Heterologous Gene Expression ◽  
Expression System ◽  
Functional Characterization ◽  
Transient Gene Expression ◽  
High Mobility ◽  
Gene Expression System

Functional characterization of plant agrichemical transporters provided an opportunity to discover molecules that have a high mobility in plants and have the potential to increase the amount of pesticides reaching damage sites. Agrobacterium-mediated transient expression in tobacco is simple and fast, and its protein expression efficiency is high; this system is generally used to mediate heterologous gene expression. In this article, transient expression of tobacco nicotine uptake permease (NtNUP1) and rice polyamine uptake transporter 1 (OsPUT1) in Nicotiana benthamiana was performed to investigate whether this system is useful as a platform for studying the interactions between plant transporters and pesticides. The results showed that NtNUP1 increases nicotine uptake in N. benthamiana foliar discs and protoplasts, indicating that this transient gene expression system is feasible for studying gene function. Moreover, yeast expression of OsPUT1 apparently increases methomyl uptake. Overall, this method of constructing a transient gene expression system is useful for improving the efficiency of analyzing the functions of plant heterologous transporter-encoding genes and revealed that this system can be further used to study the functions of transporters and pesticides, especially their interactions.

scholarly journals Selection of reference genes for gene expression analysis in Liriodendron hybrids’ somatic embryogenesis and germinative tissues

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Tingting Li ◽  
Weigao Yuan ◽  
Shuai Qiu ◽  
Jisen Shi
Keyword(s):  
Gene Expression ◽  
Somatic Embryogenesis ◽  
Reference Genes ◽  
Gene Selection ◽  
Elongation Factor ◽  
Reverse Transcription Pcr ◽  
Expression Of Genes ◽  
Suitable Reference ◽  
Functional Analyses ◽  
Elongation Factor 1

AbstractThe differential expression of genes is crucial for plant somatic embryogenesis (SE), and the accurate quantification of gene expression levels relies on choosing appropriate reference genes. To select the most suitable reference genes for SE studies, 10 commonly used reference genes were examined in synchronized somatic embryogenic and subsequent germinative cultures of Liriodendron hybrids by using quantitative real-time reverse transcription PCR. Four popular normalization algorithms: geNorm, NormFinder, Bestkeeper and Delta-Ct were used to select and validate the suitable reference genes. The results showed that elongation factor 1-gamma, histone H1 linker protein, glyceraldehyde-3-phosphate dehydrogenase and α-tubulin were suitable for SE tissues, while elongation factor 1-gamma and actin were best for the germinative organ tissues. Our work will benefit future studies of gene expression and functional analyses of SE in Liriodendron hybrids. It is also serves as a guide of reference gene selection in early embryonic gene expression analyses for other woody plant species.

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