Regional localization of the rat genes encoding the cAMP-specific phosphodiesterases 3 (Pde4d) and 4 (Pde4b) and the tyrosinase-related protein 1 (Tyrp1)

1996 ◽  
Vol 7 (3) ◽  
pp. 222-223 ◽  
Author(s):  
F. Tissir ◽  
B. Champagne ◽  
K. Klinga-Levan ◽  
G. Levan ◽  
J. Szpirer ◽  
...  
Keyword(s):  
Related Protein ◽  
Regional Localization ◽  
Genes Encoding ◽  
Tyrosinase Related Protein

scholarly journals BLOC-1 Interacts with BLOC-2 and the AP-3 Complex to Facilitate Protein Trafficking on Endosomes

2006 ◽  
Vol 17 (9) ◽  
pp. 4027-4038 ◽  
Author(s):  
Santiago M. Di Pietro ◽  
Juan M. Falcón-Pérez ◽  
Danièle Tenza ◽  
Subba R.G. Setty ◽  
Michael S. Marks ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Protein Trafficking ◽  
Adaptor Protein ◽  
Early Endosome ◽  
Related Protein ◽  
Genes Encoding ◽  
Cellular Machinery ◽  
Hermansky Pudlak Syndrome ◽  
Lysosome Related Organelles ◽  
Tyrosinase Related Protein

The adaptor protein (AP)-3 complex is a component of the cellular machinery that controls protein sorting from endosomes to lysosomes and specialized related organelles such as melanosomes. Mutations in an AP-3 subunit underlie a form of Hermansky-Pudlak syndrome (HPS), a disorder characterized by abnormalities in lysosome-related organelles. HPS in humans can also be caused by mutations in genes encoding subunits of three complexes of unclear function, named biogenesis of lysosome-related organelles complex (BLOC)-1, -2, and -3. Here, we report that BLOC-1 interacts physically and functionally with AP-3 to facilitate the trafficking of a known AP-3 cargo, CD63, and of tyrosinase-related protein 1 (Tyrp1), a melanosomal membrane protein previously thought to traffic only independently of AP-3. BLOC-1 also interacts with BLOC-2 to facilitate Tyrp1 trafficking by a mechanism apparently independent of AP-3 function. Both BLOC-1 and -2 localize mainly to early endosome-associated tubules as determined by immunoelectron microscopy. These findings support the idea that BLOC-1 and -2 represent hitherto unknown components of the endosomal protein trafficking machinery.

scholarly journals Effect of Phlorotannins Isolated From Eisenia bicyclis on Melanogenesis in Mouse B16 Melanoma Cells

2021 ◽  
Vol 16 (5) ◽  
pp. 1934578X2110192
Author(s):  
Yuki Ohno ◽  
Shiori Kondo ◽  
Kiho Tajima ◽  
Toshiyuki Shibata ◽  
Tomohiro Itoh
Keyword(s):  
Melanoma Cells ◽  
B16 Melanoma ◽  
Physiological Effects ◽  
Related Protein ◽  
Eisenia Bicyclis ◽  
Melanin Production ◽  
Melanocyte Stimulating Hormone ◽  
B16 Melanoma Cells ◽  
Anti Cancer ◽  
Tyrosinase Related Protein

Phlorotannins isolated from brown algae, such as Eisena bicyclis, have positive physiological effects, including anti-cancer, anti-inflammatory, and anti-Alzheimer’s disease. Although phlorotannins have been shown to inhibit tyrosinase, an enzyme essential for melanogenesis, their effect on melanogenesis remains unexplored. Thus, we isolated phlorotannins from E. bicyclis and examined their effects on α-melanocyte-stimulating hormone (α-MSH)-induced melanogenesis in murine B16 melanoma cells. Both fucofuroeckol-A (FF-A) and phlorofucofuroeckol-A (PFF-A) suppressed α-MSH-induced melanogenesis. Neither inhibited human tyrosinase (TYR) activity, but both inhibited tyrosinase-related protein-2 activity. FF-A downregulated the expression of microphthalmia-associated transcription factor and TYR, which subsequently suppressed melanin production. These results suggest that phlorotannins could be beneficial as melanin control drugs for hyperpigmentation disorders.

scholarly journals A Modified Tyrosinase-Related Protein 2 Epitope Generates High-Affinity Tumor-Specific T Cells but Does Not Mediate Therapeutic Efficacy in an Intradermal Tumor Model

2006 ◽  
Vol 177 (1) ◽  
pp. 155-161 ◽  
Author(s):  
Jennifer A. McWilliams ◽  
Sean M. McGurran ◽  
Steven W. Dow ◽  
Jill E. Slansky ◽  
Ross M. Kedl
Keyword(s):  
T Cells ◽  
Therapeutic Efficacy ◽  
Tumor Model ◽  
Related Protein ◽  
High Affinity ◽  
Tyrosinase Related Protein

Naturally processed and concealed HLA-A2.1 restricted epitopes from tumor associated antigen tyrosinase-related protein-2

2000 ◽  
Vol 191 (4) ◽  
pp. S71
Author(s):  
Christoph Noppen ◽  
Eugenia Remmel ◽  
Paul Zajac ◽  
Felix Harder ◽  
Giulio C Spagnoli ◽  
...  
Keyword(s):  
Related Protein ◽  
Tumor Associated Antigen ◽  
Hla A2.1 ◽  
Tyrosinase Related Protein

scholarly journals Exploring the potential effect and mechanisms of protocatechuic acid on human hair follicle melanocytes

2020 ◽  
Vol 70 (4) ◽  
pp. 539-549
Author(s):  
Bo Li ◽  
Jun Tan ◽  
Bosheng Zou ◽  
Xiaojia Liu ◽  
Yiling Yu
Keyword(s):  
Hair Follicle ◽  
Human Hair ◽  
Protocatechuic Acid ◽  
Treatment Time ◽  
Tyrosinase Activity ◽  
Dependent Manner ◽  
Related Protein ◽  
Human Hair Follicle ◽  
Dose Dependent ◽  
Tyrosinase Related Protein

AbstractThis study aims to evaluate the effect of protocatechuic acid (PCA) on human hair follicle melanocytes (HFM). Normal primary HFM were isolated and cultured till logarithmic period of second passage, then treated with different concentrations of PCA (0.1–200 μmol L−1) to study the cell proliferation, melanin contents, tyrosinase activity and protein and mRNA expression of melanogenic genes (tyrosinase-related protein 1 (TRP-1), tyrosinase-related protein 2 (TRP-2), and microphthalmia-associated transcription factor (MITF)) in the cultured HFM. In addition, we have also measured the contents of superoxide dismutase (SOD) and glutathione (GSH) in PCA treated HFM. Vitamin C was used as a positive control. The result showed that PCA can decrease the synthesis of melanin and the tyrosinase activity with IC50 = 8.9 μmol L−1 and IC50 = 6.4 μmol L−1, respectively, at the treatment time of 24 hours, without inducing any cytotoxicity in HFM cells. In addition, the mRNA transcription and protein expression levels of TRP-1, TRP-2 and MITF significantly decreased with a dose-dependent manner after 24-hour PCA treated in HFM cells. Furthermore, PCA has significantly increased the SOD and GSH activity in a dose-dependent manner for 24-hour PCA treatment. This study suggested that PCA has an inhibitory effect on the production of melanin through down-regulation of the expression of melanogenesis-related protein and the effect of anti-oxidation, which could be useful for the therapy of melanin overproduction or skin whitening.

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