Bio-inspired ZnO nanoparticles from Ocimum tenuiflorum and their in vitro antioxidant activity

Objective: In the existing study, Ocimum tenuiflorum was assessed for its antioxidant activity. This activity was assessed using the 2,2-diphenyl-1- picrylhydrazyl (DPPH) method. The drug was identified and standardized on the basis of organoleptic, negligible characters and its quantitative physicochemical standards.Methods: Preliminary phytochemical screening was also carried out to examine out the presence of various phytoconstituents. It revealed the presence of alkaloids, phytosterols, resin, flavonoids, tannins, diterpenes, and protein in the seed extract. DPPH method was done used for evaluating the antioxidant activity.Results: It has been observed that different concentrations (100, 200, 300, 400, and 500 μg/mL) used had shown 82.1%, 83.4%, 86.6%, 89.1%, and 93.2% of cell inhibition, respectively.Conclusion: Thus, the present study on pharmacognostic standardization, physicochemical evaluation and in vitro antioxidant activity of O. tenuiflorum seed would be useful to supplement information in regard to its in vivo evaluation for different pathological conditions.

Download Full-text

OPTIMIZATION AND IN VITRO EVALUATION OF CREAMS FORMULATION CONTAINING SPIRULINA (ARTHROSPIRA PLATENSIS) EXTRACT AND ZINC OXIDE NANOPARTICLES

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2021.v13s1.y0096 ◽

2021 ◽

pp. 34-37

Author(s):

ERINDYAH RETNO WIKANTYASNING ◽

KARTIKA FIDI ASTUTI ◽

ULIL FIKRI NURHAKIMAH ◽

RAMADHANI DWI SULA

Keyword(s):

Antioxidant Activity ◽

Propylene Glycol ◽

Zno Nanoparticles ◽

Arthrospira Platensis ◽

High Energy ◽

Energy Ball ◽

Ball Milling Technique ◽

Optimum Formula ◽

In Vitro Antioxidant Activity

Objective: The aims of this study are to formulate creams containing combination of spirulina (Arthrospira platensis) extract and zinc oxidenanoparticles using glycerol and propylene glycol as humectants and to evaluate their physical characteristics and in vitro antioxidant activity.Methods: Spirulina was extracted by maceration method using methanol as solvent. The high energy ball milling technique was used to obtain zincoxide nanoparticles. Creams were prepared in five formulas with the variations in the concentration of glycerol and propylene glycol. The physicalcharacteristics of creams were evaluated including pH, spreadability, adhesiveness, and viscosity. The optimization was conducted by Simplex LatticeDesign (SLD) method using Design-Expert software. In vitro antioxidant activity was evaluated using DPPH method.Results: The extraction resulted spirulina extract with a yield of 13.57%. The particle size of the ZnO nanoparticles obtained was 143.4±0.364 nm,with the polydispersity index of 0.177. Optimum formula of cream was obtained with the concentration of glycerol and propylene glycol was 12.9and 17.1%, respectively. The spreadability, adhesiveness, viscosity, and pH of the optimized cream were 12.73±0.16 cm2, 1.47±0.12 s, 126.67±5.77dPas, and 7.11±0.04, respectively. Cream containing spirulina extract and ZnO nanoparticles showed in vitro antioxidant activity with the IC50 valueof 59.47±6.34 ppm.Conclusion: Cream, containing spirulina and ZnO nanoparticles, was successfully prepared using glycerol and propylene glycol as humectant andshowed very strong antioxidant activity in vitro.

Download Full-text