Spatio-temporal characterization of double plasma mirror for ultrahigh contrast and stable laser pulse

2011 ◽  
Vol 104 (1) ◽  
pp. 81-86 ◽  
Author(s):  
IJ. Kim ◽  
I. W. Choi ◽  
S. K. Lee ◽  
K. A. Janulewicz ◽  
J. H. Sung ◽  
...  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Giulia Tedeschi ◽  
Lorenzo Scipioni ◽  
Maria Papanikolaou ◽  
Geoffrey W. Abbott ◽  
Michelle A. Digman

AbstractVoltage-gated potassium (Kv) channels are a family of membrane proteins that facilitate K+ ion diffusion across the plasma membrane, regulating both resting and action potentials. Kv channels comprise four pore-forming α subunits, each with a voltage sensing domain, and they are regulated by interaction with β subunits such as those belonging to the KCNE family. Here we conducted a comprehensive biophysical characterization of stoichiometry and protein diffusion across the plasma membrane of the epithelial KCNQ1-KCNE2 complex, combining total internal reflection fluorescence (TIRF) microscopy and a series of complementary Fluorescence Fluctuation Spectroscopy (FFS) techniques. Using this approach, we found that KCNQ1-KCNE2 has a predominant 4:4 stoichiometry, while non-bound KCNE2 subunits are mostly present as dimers in the plasma membrane. At the same time, we identified unique spatio-temporal diffusion modalities and nano-environment organization for each channel subunit. These findings improve our understanding of KCNQ1-KCNE2 channel function and suggest strategies for elucidating the subunit stoichiometry and forces directing localization and diffusion of ion channel complexes in general.


2021 ◽  
pp. 116927
Author(s):  
Bruna de Ramos ◽  
Melanie Vianna Alencar ◽  
Fábio Lameiro Rodrigues ◽  
Ana Luzia de Figueiredo Lacerda ◽  
Maíra Carneiro Proietti

2021 ◽  
Vol 11 (12) ◽  
pp. 5424
Author(s):  
Itamar Cohen ◽  
Yonatan Gershuni ◽  
Michal Elkind ◽  
Guy Azouz ◽  
Assaf Levanon ◽  
...  

The versatility of laser accelerators in generating particle beams of various types is often promoted as a key applicative advantage. These multiple types of particles, however, are generated on vastly different irradiation setups, so that switching from one type to another involves substantial mechanical changes. In this letter, we report on a laser-based accelerator that generates beams of either multi-MeV electrons or ions from the same thin-foil irradiation setup. Switching from generation of ions to electrons is achieved by introducing an auxiliary laser pulse, which pre-explodes the foil tens of ns before irradiation by the main pulse. We present an experimental characterization of the emitted beams in terms of energy, charge, divergence, and repeatability, and conclude with several examples of prospective applications for industry and research.


2016 ◽  
Vol 87 (12) ◽  
pp. 123105 ◽  
Author(s):  
J. Zhang ◽  
X. H. Shi ◽  
X. Y. Zeng ◽  
X. L. Lü ◽  
K. Deng ◽  
...  

2021 ◽  
Vol 58 (2) ◽  
pp. 281-299
Author(s):  
Somnath Bar ◽  
Bikash Ranjan Parida ◽  
Gareth Roberts ◽  
Arvind Chandra Pandey ◽  
Prasenjit Acharya ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document