Prevalence of human papillomavirus (HPV) among college going girls using self collected urine samples from Tiruchirappalli, Tamilnadu

To test a new duplex nanoparticle-assisted polymerase chain reaction (PCR) on human papillomavirus (HPV) E6 oncoprotein detection of HPV16/18 positive samples from vaginal and urine self-sampling specimen. Six specimens from cervical biopsy ranging from CIN1 to CIN3, which have been testified by conventional PCR, were used for nanoPCR HPV E6 oncoprotein specificity test. Seven serially diluted templates by ten-fold (3.78?106?3.78?100 copies/μL) chosen from testified CIN3 biopsy were selected for the sensitivity test. Twenty self-sampling vaginal and urine samples each from 20 women in our hospital were tested by nanoPCR and conventional PCR. NanoPCR showed high specificity when detecting HPV16/18 infected CIN2 and CIN3 samples from cervical biopsy and ten-fold higher sensitivity than conventional PCR when detecting HPV16/18 infected CIN3 samples from cervical biopsy. DNA gel electrophoresis showed brighter bands on positive HPV E6 oncoprotein specimen from self-collected vaginal and urine samples compared to conventional PCR and higher clinical detection rate than those by conventional PCR on vaginal and urine self-sampling specimens. Duplex nanoPCR can be used as a better method for HPV E6 oncoprotein detection in the high-risk HPV infection group for its novelty of high specificity in low concentration target detection, as was showed in the specimen comes from vaginal and urine self-sampling, thus promoting self-sampling for better cervical cancer screening.

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Test Accuracy of Human Papillomavirus in Urine for Detection of Cervical Intraepithelial Neoplasia

Journal of Clinical Microbiology ◽

10.1128/jcm.01443-19 ◽

2020 ◽

Vol 58 (3) ◽

Cited By ~ 2

Author(s):

Eliane Rohner ◽

Lisa Rahangdale ◽

Busola Sanusi ◽

Andrea K. Knittel ◽

Laurence Vaughan ◽

...

Keyword(s):

Human Papillomavirus ◽

Cervical Intraepithelial Neoplasia ◽

Access To Health Care ◽

Intraepithelial Neoplasia ◽

Urine Samples ◽

Test Accuracy ◽

Sample Collection ◽

Cervical Intraepithelial Neoplasia Grade ◽

Normal Cytology ◽

Convenience Sample

ABSTRACT The objective was to assess the diagnostic test accuracy of high-risk human papillomavirus (hrHPV) testing of self-collected urine and cervicovaginal samples for the detection of cervical intraepithelial neoplasia grade 2 or higher (CIN2+). We recruited a convenience sample of women 25 to 65 years of age who were undergoing clinically indicated colposcopy at two medical centers in North Carolina between November 2016 and January 2019. Women with normal cytology results and positive hrHPV results were also recruited. Urine samples, self-collected cervicovaginal samples, provider-collected cervical samples, and cervical biopsy samples were obtained from all enrolled women. Samples were tested for hrHPV DNA using the Onclarity assay (Becton Dickinson, Sparks, MD). Biopsy samples were histologically graded as CIN2+ or <CIN2. We calculated the sensitivity and specificity for detection of CIN2+ and assessed agreement between sample collection methods. We included 307 women (median age, 36 years) with valid histology results and triple-matched urine, self-collected cervicovaginal, and provider-collected cervical hrHPV results; 83 women (27%) had CIN2+. Urine-based hrHPV testing correctly identified 80% of CIN2+ cases (95% confidence interval [CI], 71 to 88%) using the PCR cycle threshold (CT) established for provider-collected cervical samples, but sensitivity remained below the estimates for self-collected cervicovaginal and provider-collected cervical samples (both 94% [95% CI, 89 to 99%]). Using a higher CT cutoff value of ≤40, 90% sensitivity was achieved for urine-based hrHPV testing. Agreement between results for urine samples and self-collected cervicovaginal samples (kappa = 0.58) or provider-collected cervical samples (kappa = 0.54) was moderate. Urine-based hrHPV testing may be a promising approach to improve cervical cancer screening coverage, especially among women with limited access to health care.

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