Histochemical, ultrastructural, and three-dimensional observation of smooth muscle cells in human gastric mucosa

2004 ◽  
Vol 121 (3) ◽  
pp. 229-237 ◽  
Author(s):  
Tsutomu Katsuyama ◽  
Kenichi Arai ◽  
Hiroyoshi Ota ◽  
Eiko Hidaka ◽  
Masayoshi Hayama ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Gastric Mucosa ◽  
Smooth Muscle Cells ◽  
Three Dimensional ◽  
Muscle Cells ◽  
Human Gastric Mucosa

scholarly journals ULTRASTRUCTURAL INTERRELATIONSHIPS OF NERVE PROCESSES AND SMOOTH MUSCLE CELLS IN THREE DIMENSIONS

1966 ◽  
Vol 28 (1) ◽  
pp. 37-49 ◽  
Author(s):  
J. C. Thaemert
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Osmium Tetroxide ◽  
Three Dimensional ◽  
Muscle Cells ◽  
Uranyl Acetate ◽  
Intestinal Wall ◽  
Three Dimensions ◽  
Serial Sections ◽  
Muscularis Externa

The muscularis externa of the intestinal wall of frogs was fixed in osmium tetroxide, embedded in Vestopal-W, serially sectioned for electron microscopy, and stained with uranyl acetate. A method to obtain individually mounted and properly positioned serial sections is described. The three-dimensional techniques used during the course of this investigation demonstrate that it is possible to examine carefully relatively large areas of tissue on individual serial sections with the electron microscope and subsequently to construct montages of electron micrographs of pertinent areas from each section. Several carefully rendered interrelationships of nerve processes and smooth muscle cells in three dimensions are exhibited and described. Recent studies of other neuro-effector relationships are discussed in relation to the present status of the nature and organization of the autonomic nervous system in visceral organs.

Mechanical Stress-Induced Orientation and Ultrastructural Change of Smooth Muscle Cells Cultured in Three-Dimensional Collagen Lattices

1994 ◽  
Vol 3 (6) ◽  
pp. 481-492 ◽  
Author(s):  
Keiichi Kanda ◽  
Takehisa Matsuda
Keyword(s):  
Smooth Muscle ◽  
Tensile Stress ◽  
Smooth Muscle Cells ◽  
Type I Collagen ◽  
Dynamic Stress ◽  
Three Dimensional ◽  
Muscle Cells ◽  
The Other ◽  
Type I ◽  
Oriented Parallel

The effect of tensile stress on the orientation and phenotype of arterial smooth muscle cells (SMCs) cultured in three-dimensional (3D) type I collagen gels was morphologically investigated. Ring-shaped hybrid tissues were prepared by thermal gelation of a cold mixed solution of type I collagen and SMCs derived from bovine aorta. The tissues were subjected to three different modes of tensile stress. They were floated (isotonic control), stretched isometrically (static stress) and periodically stretched and recoiled by 5% above and below the resting tissue length at 60 RPM frequency (dynamic stress). After incubation for up to four wk, the tissues were investigated under a light microscope (LM) and a transmission electron microscope (TEM). Hematoxylin and eosinstained LM samples revealed that, irrespective of static or dynamic stress loading, SMCs in stress-loaded tissues exhibited elongated bipolar spindle shape and were regularly oriented parallel to the direction of the strain, whereas those in isotonic control tissues were polygonal or spherical and had no preferential orientation. In Azan-stained samples, collagen fiber bundles in isotonic control tissues were somewhat retracted around the polygonal SMCs to form a random network. On the other hand, those in statically and dynamically stressed tissues were accumulated and prominently oriented parallel to the stretch direction. Ultrastructural investigation using a TEM showed that SMCs in control and statically stressed tissues were almost totally filled with synthetic organelles such as rough endoplasmic reticulums, free ribosomes, Golgi complexes and mitochondria, indicating that the cells remained in the synthetic phenotype. On the other hand, SMCs in dynamically stressed tissues had increased fractions of contractile apparatus, such as myofilaments, dense bodies and extracellular filamentous materials equivalent to basement membranes, that progressed with incubation time. These results indicate that periodic stretch, in concert with 3-D extracellular collagen matrices, play a significant role in the phenotypic modulation of SMCs from the synthetic to the contractile state, as well as cellular and biomolecular orientation.

Differentiating human pluripotent stem cells into vascular smooth muscle cells in three dimensional thermoreversible hydrogels

2019 ◽  
Vol 7 (1) ◽  
pp. 347-361 ◽  
Author(s):  
Haishuang Lin ◽  
Qian Du ◽  
Qiang Li ◽  
Ou Wang ◽  
Zhanqi Wang ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Pluripotent Stem Cells ◽  
Pluripotent Stem Cell ◽  
Three Dimensional ◽  
Muscle Cells ◽  
Human Pluripotent Stem Cell ◽  
Scalable Production

3D thermoreversible PNIPAAm-PEG hydrogels are used for scalable production of human pluripotent stem cell-derived vascular smooth muscle cells.

Fabrication of in vitro three-dimensional multilayered blood vessel model using human endothelial and smooth muscle cells and high-strength PEG hydrogel

2013 ◽  
Vol 116 (2) ◽  
pp. 231-234 ◽  
Author(s):  
Sho Shinohara ◽  
Takanori Kihara ◽  
Shinji Sakai ◽  
Michiya Matsusaki ◽  
Mitsuru Akashi ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Blood Vessel ◽  
Smooth Muscle Cells ◽  
Three Dimensional ◽  
High Strength ◽  
Muscle Cells

Three-Dimensional Microchannels in Biodegradable Polymeric Films for Control Orientation and Phenotype of Vascular Smooth Muscle Cells

2006 ◽  
Vol 0 (0) ◽  
pp. 060913044658036
Author(s):  
Jin Ye Shen ◽  
Mary B. Chan-Park ◽  
B. He ◽  
Ai Ping Zhu ◽  
Xiao Zhu ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Three Dimensional ◽  
Muscle Cells ◽  
Polymeric Films ◽  
Control Orientation

Morphology of vascular smooth muscle cells and pericytes as revealed by transmission and scanning EM

1978 ◽  
Vol 36 (2) ◽  
pp. 464-465
Author(s):  
Y. Uehara ◽  
T. Komuro ◽  
J. Desaki
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cell ◽  
Vascular Function ◽  
Three Dimensional ◽  
Muscle Cells ◽  
Structural Level ◽  
Scanning Em

Although subcellular organization of vascular smooth muscle cells and pericytes have been described by a number of authors, the precise overall structure of these cells has not been revealed at the fine structural level.In order to extend our knowledge in understanding of vascular function, the present paper deals the three dimensional organization of these cells as studied with a transmission EM using serial sections and with a scanning EM using materials from which connective tissue elements are removed.For the reconstruction study, arterioles ranging in diameter from l0um to 25um are selected from the hamster skeletal muscle and the rat pancreas.Fig. 1. demonstrates an example of the reconstructed model of the arteriole smooth muscle cell. Here the muscle cell takes a spiral form which coils nearly twice around the endothelial column. The shape and disposition of the muscle cells gradually become to be irregular towards the capillary bed.

Three-dimensional organization of smooth muscle cells in blood vessels of laboratory rodents

1982 ◽  
Vol 227 (2) ◽  
pp. 429-437 ◽  
Author(s):  
Terumasa Komuro ◽  
Junzo Desaki ◽  
Yasuo Uehara
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Blood Vessels ◽  
Three Dimensional ◽  
Muscle Cells ◽  
Laboratory Rodents
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