Cell cycle-dependent subcellular distribution of ClC-3 in HeLa cells

2012 ◽  
Vol 137 (6) ◽  
pp. 763-776 ◽  
Author(s):  
Jianwen Mao ◽  
Xiaobo Li ◽  
Weiqiang Chen ◽  
Bin Xu ◽  
Haifeng Zhang ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Hela Cells ◽  
Subcellular Distribution ◽  
Cycle Dependent

scholarly journals Cyclin B2 undergoes cell cycle-dependent nuclear translocation and, when expressed as a non-destructible mutant, causes mitotic arrest in HeLa cells

1992 ◽  
Vol 117 (1) ◽  
pp. 213-224 ◽  
Author(s):  
P Gallant ◽  
EA Nigg
Keyword(s):  
Cell Cycle ◽  
Hela Cells ◽  
Subcellular Distribution ◽  
Nuclear Translocation ◽  
Mitotic Spindles ◽  
Cycle Arrest ◽  
Regulatory Machinery ◽  
Essential Components ◽  
Cycle Dependent ◽  
Monospecific Antibodies

Cyclin proteins form complexes with members of the p34cdc2 kinase family and they are essential components of the cell cycle regulatory machinery. They are thought to determine the timing of activation, the subcellular distribution, and/or the substrate specificity of cdc2-related kinases, but their precise mode of action remains to be elucidated. Here we report the cloning and sequencing of avian cyclin B2. Based on the use of monospecific antibodies raised against bacterially expressed protein, we also describe the subcellular distribution of cyclin B2 in chick embryo fibroblasts and in DU249 hepatoma cells. By indirect immunofluorescence microscopy we show that cyclin B2 is cytoplasmic during interphase of the cell cycle, but undergoes an abrupt translocation to the cell nucleus at the onset of mitotic prophase. Finally, we have examined the phenotypic consequences of expressing wild-type and mutated versions of avian cyclin B2 in HeLa cells. We found that expression of cyclin B2 carrying a mutation at arginine 32 (to serine) caused HeLa cells to arrest in a pseudomitotic state. Many of the arrested cells displayed multiple mitotic spindles, suggesting that the centrosome cycle had continued in spite of the cell cycle arrest.

Cell-cycle dependent subcellular distribution and mitogenic effects of PTOV1, a novel protein overexpressed in prostate cancer

2003 ◽  
Vol 2 (1) ◽  
pp. 161
Author(s):  
A. Santamaria ◽  
P. Fernandez ◽  
X. Farre ◽  
P. Benedit ◽  
Joan Morote ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cell Cycle ◽  
Subcellular Distribution ◽  
Cycle Dependent ◽  
Novel Protein

Periodic biosynthesis of the human M-phase promoting factor catalytic subunit p34 during the cell cycle

1990 ◽  
Vol 10 (7) ◽  
pp. 3847-3851
Author(s):  
C H McGowan ◽  
P Russell ◽  
S I Reed
Keyword(s):  
Cell Cycle ◽  
Protein Kinase ◽  
Hela Cells ◽  
Catalytic Subunit ◽  
Dependent Manner ◽  
Reversible Phosphorylation ◽  
M Phase ◽  
A Cell ◽  
Cycle Dependent

The product of the CDC2Hs gene is the protein kinase subunit of the M-phase promoting factor, which is required for entry into mitosis. The activity of this kinase is regulated in a cell cycle-dependent manner by reversible phosphorylation and through association with other proteins. We report here that in HeLa cells, the abundance of the CDC2Hs mRNA and the rate of synthesis of the encoded protein, p34, vary in a cell cycle-dependent manner.

Tubulin polyglutamylase: isozymic variants and regulation during the cell cycle in HeLa cells

1999 ◽  
Vol 112 (23) ◽  
pp. 4281-4289 ◽  
Author(s):  
C. Regnard ◽  
E. Desbruyeres ◽  
P. Denoulet ◽  
B. Edde
Keyword(s):  
Cell Cycle ◽  
Hela Cells ◽  
Molecular Motors ◽  
Dependent Manner ◽  
Proliferating Cells ◽  
Beta Tubulin ◽  
Microtubule Associated Proteins ◽  
Associated Proteins ◽  
A Cell ◽  
Cycle Dependent

Polyglutamylation is a posttranslational modification of tubulin that is very common in neurons and ciliated or flagellated cells. It was proposed to regulate the binding of microtubule associated proteins (MAPs) and molecular motors as a function of the length of the polyglutamyl side-chain. Though much less common, this modification of tubulin also occurs in proliferating cells like HeLa cells where it is associated with centrioles and with the mitotic spindle. Recently, we partially purified tubulin polyglutamylase from mouse brain and described its enzymatic properties. In this work, we focused on tubulin polyglutamylase activity from HeLa cells. Our results support the existence of a tubulin polyglutamylase family composed of several isozymic variants specific for alpha- or beta-tubulin subunits. In the latter case, the specificity probably also concerns the different beta-tubulin isotypes. Interestingly, we found that tubulin polyglutamylase activity is regulated in a cell cycle dependent manner and peaks in G(2)-phase while the level of glutamylated tubulin peaks in mitosis. Consistent results were obtained by treating the cells with hydroxyurea, nocodazole or taxotere. In particular, in mitotic cells, tubulin polyglutamylase activity was always low while glutamylation level was high. Finally, tubulin polyglutamylase activity and the level of glutamylated tubulin appeared to be inversely related. This paradox suggests a complex regulation of both tubulin polyglutamylase and the reverse deglutamylase activity.

scholarly journals Cell cycle regulation of the p34cdc2 inhibitory kinases.

1994 ◽  
Vol 5 (9) ◽  
pp. 989-1001 ◽  
Author(s):  
S Atherton-Fessler ◽  
F Liu ◽  
B Gabrielli ◽  
M S Lee ◽  
C Y Peng ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Hela Cells ◽  
Cell Cycle Regulation ◽  
Dependent Manner ◽  
Dual Specificity ◽  
Egg Extracts ◽  
A Cell ◽  
Cycle Dependent ◽  
Eukaryotic Organisms ◽  
Cycle Regulation

In cells of higher eukaryotic organisms the activity of the p34cdc2/cyclin B complex is inhibited by phosphorylation of p34cdc2 at two sites within its amino-terminus (threonine 14 and tyrosine 15). In this study, the cell cycle regulation of the kinases responsible for phosphorylating p34cdc2 on Thr14 and Tyr15 was examined in extracts prepared from both HeLa cells and Xenopus eggs. Both Thr14- and Tyr15- specific kinase activities were regulated in a cell cycle-dependent manner. The kinase activities were high throughout interphase and diminished coincident with entry of cells into mitosis. In HeLa cells delayed in G2 by the DNA-binding dye Hoechst 33342, Thr14- and Tyr15-specific kinase activities remained high, suggesting that a decrease in Thr14- and Tyr15- kinase activities may be required for entry of cells into mitosis. Similar cell cycle regulation was observed for the Thr14/Tyr15 kinase(s) in Xenopus egg extracts. These results indicate that activation of CDC2 and entry of cells into mitosis is not triggered solely by activation of the Cdc25 phosphatase but by the balance between Thr14/Tyr15 kinase and phosphatase activities. Finally, we have detected two activities capable of phosphorylating p34cdc2 on Thr14 and/or Tyr15 in interphase extracts prepared from Xenopus eggs. An activity capable of phosphorylating Tyr15 remained soluble after ultracentrifugation of interphase extracts whereas a second activity capable of phosphorylating both Thr14 and Tyr15 pelleted. The pelleted fraction contained activities that were detergent extractable and that phosphorylated p34cdc2 on both Thr14 and Tyr15. The Thr14- and Tyr15-specific kinase activities co-purified through three successive chromatographic steps indicating the presence of a dual-specificity protein kinase capable of acting on p34cdc2.

scholarly journals Periodic biosynthesis of the human M-phase promoting factor catalytic subunit p34 during the cell cycle.

1990 ◽  
Vol 10 (7) ◽  
pp. 3847-3851 ◽  
Author(s):  
C H McGowan ◽  
P Russell ◽  
S I Reed
Keyword(s):  
Cell Cycle ◽  
Protein Kinase ◽  
Hela Cells ◽  
Catalytic Subunit ◽  
Dependent Manner ◽  
Reversible Phosphorylation ◽  
M Phase ◽  
A Cell ◽  
Cycle Dependent

The product of the CDC2Hs gene is the protein kinase subunit of the M-phase promoting factor, which is required for entry into mitosis. The activity of this kinase is regulated in a cell cycle-dependent manner by reversible phosphorylation and through association with other proteins. We report here that in HeLa cells, the abundance of the CDC2Hs mRNA and the rate of synthesis of the encoded protein, p34, vary in a cell cycle-dependent manner.

Molecular Cloning, Chromosomal Localization, and Cell Cycle-Dependent Subcellular Distribution of the A-Kinase Anchoring Protein, AKAP95

1998 ◽  
Vol 238 (2) ◽  
pp. 305-316 ◽  
Author(s):  
Turid Eide ◽  
Vince Coghlan ◽  
Sigurd Ørstavik ◽  
Christian Holsve ◽  
Rigmor Solberg ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Molecular Cloning ◽  
Subcellular Distribution ◽  
Chromosomal Localization ◽  
Anchoring Protein ◽  
Cycle Dependent
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