The present paper reports on three sets of experiments exploring the persistence of seeds of oilseed rape (Brassica napus). The first, where known numbers of seeds were buried in September 1991 in two field experiments, demonstrated substantial initial losses of seeds, such that only 0·2 and 3·8% of seeds were still present after 4 months. In these experiments, which were not disturbed by mechanical cultivation, there was little evidence of further decline over the following 13 months. In the second of the two experiments, seeds were then left undisturbed for a further 136 months. A mean of 1·8% of seeds were still present after this period, providing further confirmation of the lack of decline in seed numbers in these undisturbed conditions. In the second pair of experiments, known numbers of seeds of three rape cultivars were broadcast onto plots and then either ploughed into the soil immediately after the start of the experiments, or were exposed to weekly shallow tine cultivation followed by ploughing after 4 weeks. The former created a larger seedbank than the latter. The experiments were then ploughed, annually (Expt 1) or at less frequent intervals (Expt 2); appreciable numbers of seeds survived for 65 months in both. Calculations based on exponential decline curves indicated that 95% seed loss would take 15–39 months, depending on the site, cultivar and initial post-harvest stubble treatment. The third part of the paper is based on more detailed studies of persistence of seeds of six cultivars in Petri dishes and buried in 25 cm pots. This work confirmed that cultivars differed in their persistence, as Apex was confirmed as highly persistent, whereas Rebel was short-lived. There were inconsistencies in the response of cultivar Synergy between the Petri-dish and pot experiment, which need further study. This experiment also reinforced the conclusion of the initial field experiments that little seed loss occurs in the absence of cultivations. Appreciable numbers of rape seeds will persist up to 4 years, in normal cropping conditions and in the absence of cultivation one experiment has confirmed persistence for over 11 years.
Functional Analysis of Organic Acids on Different Oilseed Rape Species in Phytoremediation of Cadmium Pollution
