Manipulation of DXP pathway for andrographolide production in callus cultures of Andrographis paniculata

Planta ◽  
2021 ◽  
Vol 254 (2) ◽  
Author(s):  
Debalina Das ◽  
Maumita Bandyopadhyay
Keyword(s):  
Callus Cultures ◽  
Andrographis Paniculata

Formation of three new flavones by differentiating callus cultures of andrographis paniculata

1979 ◽  
Vol 18 (1) ◽  
pp. 149-151 ◽  
Author(s):  
Mahbubul A.F. Jalal ◽  
Karl H. Overton ◽  
David S. Rycroft
Keyword(s):  
Callus Cultures ◽  
Andrographis Paniculata

SEM and fluorescence imaging of callose deposition in root hair tips and suspension cultures of Streptanthus tortuosus

1990 ◽  
Vol 48 (3) ◽  
pp. 698-699
Author(s):  
K.S. Walters ◽  
R.D. Sjolund ◽  
K.C. Moore
Keyword(s):  
Cell Wall ◽  
Root Hair ◽  
Cultured Cells ◽  
Root Hairs ◽  
Callus Cultures ◽  
Callose Deposition ◽  
Culture Cells ◽  
Wall Structures ◽  
Ultraviolet Illumination ◽  
Callose Formation

Callose, B-1,3-glucan, a component of cell walls, is associated with phloem sieve plates, plasmodesmata, and other cell wall structures that are formed in response to wounding or infection. Callose reacts with aniline blue to form a fluorescent complex that can be recognized in the light microscope with ultraviolet illumination. We have identified callose in cell wall protuberances that are formed spontaneously in suspension-cultured cells of S. tortuosus and in the tips of root hairs formed in sterile callus cultures of S. tortuosus. Callose deposits in root hairs are restricted to root hair tips which appear to be damaged or deformed, while normal root hair tips lack callose deposits. The callose deposits found in suspension culture cells are restricted to regions where unusual outgrowths or protuberances are formed on the cell surfaces, specifically regions that are the sites of new cell wall formation.Callose formation has been shown to be regulated by intracellular calcium levels.

PENGARUH EKSTRAK SAMBILOTO (Andrographis paniculata [Burm f..] Ness) TERHADAP HITUNG EOSINOFIL MENCIT MODEL ALERGI INFLAMASI

2012 ◽  
Vol 12 (1) ◽  
Author(s):  
Arisanty Nursetia R
Keyword(s):  
Andrographis Paniculata ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
Per Oral

Sambiloto mengandung flavonoid dan andrografolid yang mempunyai efek anti alergi. Penelitian ini bertujuan untuk mengetahui Pengaruh Ekstrak Sambiloto (Andrographis paniculata [Burm f.] Ness) terhadap hitung Eosinofil Serum Mencit Model Alergi Inflamasi. Jenis penelitian ini adalah eksperimental laboratorik, dengan post test only control group design. Hewan uji berupa 15 ekor mencit jantan. Mencit dibagi menjadi tiga kelompok, masing-masing terdiri dari 5 ekor. Kelompok I (kontrol) hanya diberi diet standar. Kelompok II diberi diet standar dan ekstrak sambiloto per oral dengan dosis 10mg/20 gram BB/hari. Kelompok III diberi diet standar dan antihistamin per-oral dengan dosis 0,4 mg/20 gram BB/hari. Sensitisasi mencit dengan ovalbumin (OVA). Hari ke-4 dan ke-20 dipapar OVA i.p. Dosis 0,15 cc/mencit. OVA inhalasi ke-37 secara intraorbital. Analisis data dengan uji Anova menggunakan program SPSS for Windows Release 11.5. Hasil penelitian menunjukkan rata-rata eosinofil mencit kelompok I = 0,94% kelompok II = 0,87% dan kelompok III = 1,2%. Dengan analisa statistik kelompok II dibandingkan kelompok I, kelompok III dibandingkan kelompok I dan kelompok II dibanding kelompok III menunjukkan perbedaan hitung eosinofil yang tidak bermakna (p>0,05). Ekstrak sambiloto dengan dosis 10 mg/20gram BB/hari dapat menurunkan hitung eosinofil serum pada mencit model alergi inflasi.

In Vitro Establishment of Powdery Mildew (Microsphaera pulchra) on Microshoots of Flowering Dogwood

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 506a-506
Author(s):  
L.A. Klein ◽  
M.T. Windham ◽  
R.N. Trigiano
Keyword(s):  
Powdery Mildew ◽  
Woody Plant ◽  
Infected Individual ◽  
Callus Cultures ◽  
Fungal Culture ◽  
Culture Collections ◽  
Flowering Dogwood ◽  
Plant Parasite ◽  
In Vitro Establishment

Microshoot and callus cultures of Cornus florida (flowering dogwood), which were grown on woody plant medium amended with BA, were inoculated with Microsphaera pulchra (an obligate plant parasite) by gently shaking infected leaves bearing numerous conidia over the tissue. Culture dishes were sealed with parafilm and incubated at 24 °C with 25 mol·m–2·s–1 provided by cool fluorescent bulbs for 15 h. Cultures were examined with a dissecting scope every 24 h and cultures transferred when contaminating fungi were present. Specimens were prepared light microscopy and SEM. The fungus infected individual callus cells, but did not sporulate. In contrast, powdery mildew was well-established (both primary and secondary hyphae) in 70% of the microshoot cultures after 6 days and sporulated on 20% by 7 to 8 days. The cellular relationship between host and pathogen in vitro was similar to that found in greenhouse-grown plants. This technique has possible applications in maintaining fungal culture collections and studying host–pathogen relationships under more stringently controlled conditions.

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