Human tissue transglutaminase enzyme linked immunosorbent assay outperforms both the guinea pig based tissue transglutaminase assay and anti-endomysium antibodies when screening for coeliac disease

2002 ◽  
Vol 161 (5) ◽  
pp. 284-287 ◽  
Author(s):  
Victorien Wolters ◽  
Anne-Françoise Vooijs-Moulaert ◽  
Huib Burger ◽  
Rik Brooimans ◽  
Jan De Schryver ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Coeliac Disease ◽  
Immunosorbent Assay ◽  
Human Tissue ◽  
Tissue Transglutaminase ◽  
Enzyme Linked Immunosorbent Assay ◽  
Endomysium Antibodies

A comparative study of tissue transglutaminase antibodies and endomysium antibody immunofluorescence in routine clinical laboratory practice

2003 ◽  
Vol 40 (4) ◽  
pp. 411-416 ◽  
Author(s):  
David Sinclair ◽  
Callum B Pearce ◽  
Michael S. L. Saas ◽  
David Poller
Keyword(s):  
Guinea Pig ◽  
Coeliac Disease ◽  
Clinical Laboratory ◽  
Tissue Transglutaminase ◽  
Iga Deficiency ◽  
Complete Agreement ◽  
Tissue Transglutaminase Antibodies ◽  
Routine Clinical Laboratory ◽  
Endomysium Antibodies ◽  
Human Source

Background: The demand for screening for coeliac disease has grown rapidly over the last few years. Laboratories depending on immunofluorescence assays are faced with an increasing workload using a labour-intensive test, and an alternative to this test has been sought. This study compares tissue transglutaminase (TTG) and endomysium antibodies (EMA) in a routine clinical laboratory situation. Methods: An immunofluorescence IgA EMA test was compared with a guinea pig TTG antibody ELISA for 816 unselected requests for gut antibody screening. Discrepant results were investigated more fully using a variety of human source TTG antigen kits. Results: Guinea pig TTG ELISA and EMA assays showed agreement for 93·6% of samples. Four samples were misclassified and 48 samples gave false positive TTG results. Study of 46 EMA samples (this group included 39 of the 'discrepant' negative EMA/positive guinea pig TTG group) using three different human purified and/or recombinant TTG sources showed that 42 patients had no TTG antibodies using human sources, three were misclassified and one patient had negative EMA and positive TTG results that could not be readily explained. Further study of 32 EMA positive samples showed almost complete agreement between the human source TTG kits. Conclusions: We can recommend the replacement of EMA with ELISA for TTG antibodies for the routine screening for coeliac disease, but all positive TTG antibodies should still be followed up with IgA EMA and samples should be screened for IgA deficiency.

Quantification of human tissue transglutaminase by a luminescence sandwich enzyme-linked immunosorbent assay

2011 ◽  
Vol 419 (2) ◽  
pp. 153-160 ◽  
Author(s):  
Johannes Wolf ◽  
Ingolf Lachmann ◽  
Uta Wagner ◽  
Awad A. Osman ◽  
Thomas Mothes
Keyword(s):  
Immunosorbent Assay ◽  
Human Tissue ◽  
Tissue Transglutaminase ◽  
Enzyme Linked Immunosorbent Assay

Preparation of Antibodies to Guinea Pig IgE and Its Use for Enzyme-Linked Immunosorbent Assay of IgE Antibodies

1985 ◽  
Vol 77 (4) ◽  
pp. 438-444 ◽  
Author(s):  
Koji Ito ◽  
Nobukazu Yamauchi ◽  
Shunsuke Shoji ◽  
Yasubumi Miyamoto ◽  
Terumasa Miyamoto ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Ige Antibodies

Assay of Human Tissue-Type Plasminogen Activator (t-PA) with an Enzyme-Linked Immunosorbent Assay (ELISA) Based on Three Murine Monoclonal Antibodies to t-PA

1985 ◽  
Vol 54 (03) ◽  
pp. 684-687 ◽  
Author(s):  
Paul Holvoet ◽  
Hugo Cleemput ◽  
Désiré Collen
Keyword(s):  
Monoclonal Antibodies ◽  
Plasminogen Activator ◽  
Immunosorbent Assay ◽  
Human Tissue ◽  
Biological Fluids ◽  
Human Subjects ◽  
Tissue Type ◽  
Enzyme Linked Immunosorbent Assay ◽  
Tissue Type Plasminogen Activator ◽  
Type Plasminogen Activator

SummaryAn enzyme-linked immunosorbent assay (ELISA) for the measurement of human tissue-type plasminogen activator (t-PA) was developed. Microtiter plates were coated with a mixture of two monoclonal antibodies and bound t-PA was quantitated with a third monoclonal antibody linked to peroxidase. The lower limit of sensitivity of the assay was 0.2 ng of t-PA per ml. The concentration of antigen in citrated plasma of human subjects was found to be 3.4 ± 0.8 ng/ml. The assay had a good reproducibility with values of 3.8, 6.5 and 4.9 percent respectively for the intra-, inter-assay and inter-dilution variation coefficients. The results of the ELISA assay on plasma samples from patients during thrombolytic therapy with t-PA correlated very well, over a wide concentration range, with those obtained with a previously described two-site immuno-radiometric assay (r = 0.96). This ELISA with monoclonal antibodies constitutes a stable and reproducible set of reagents for the measurement of t-PA antigen in biological fluids, avoiding the disadvantages of the use of radioisotopes and of polyclonal antibodies.

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