An enzyme-linked immunosorbent assay (ELISA) for anti-chlamydial secretory immunoglobulin A in guinea pig tears

1986 ◽  
Vol 86 (1) ◽  
pp. 71-74 ◽  
Author(s):  
P.M. Finney ◽  
A.C. Bushell
Keyword(s):  
Guinea Pig ◽  
Immunosorbent Assay ◽  
Immunoglobulin A ◽  
Enzyme Linked Immunosorbent Assay ◽  
Secretory Immunoglobulin A ◽  
Secretory Immunoglobulin

scholarly journals Fecal Immunoglobulin A Antibodies in Dogs Infected or Vaccinated with Canine Coronavirus

2004 ◽  
Vol 11 (1) ◽  
pp. 102-105 ◽  
Author(s):  
Nicola Decaro ◽  
Annamaria Pratelli ◽  
Antonella Tinelli ◽  
Vito Martella ◽  
Michele Camero ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Immunoglobulin A ◽  
Enzyme Linked Immunosorbent Assay ◽  
Inactivated Vaccine ◽  
Secretory Immunoglobulin A ◽  
Intramuscular Route ◽  
Degree Of Protection ◽  
Canine Coronavirus ◽  
Iga Antibodies ◽  
Secretory Immunoglobulin

ABSTRACT Fecal secretory immunoglobulin A (IgA) antibodies in dogs infected or vaccinated with canine coronavirus (CCV) were evaluated by an enzyme-linked immunosorbent assay. The study was carried out with 32 fecal samples collected just before inoculation and at 28 days postinoculation. Five groups were studied: naturally infected dogs, experimentally infected dogs, dogs inoculated with a modified live (ML) CCV vaccine by the intramuscular route, dogs inoculated with an ML CCV vaccine by the oronasal route, and dogs given an inactivated CCV vaccine. Both the naturally and the experimentally infected dogs developed high levels of fecal IgAs. Interestingly, dogs inoculated with the ML CCV vaccine by the oronasal route developed levels of fecal IgA that were higher than those observed in the dogs inoculated with the same CCV vaccine by the intramuscular route or those observed in dogs inoculated with the inactivated vaccine. A relationship between the level of fecal IgAs to CCV and the degree of protection against CCV infection was observed.

A quantitative enzyme-linked immunosorbent assay for quantification of secretory immunoglobulin A in serum

2016 ◽  
Vol 38 (1) ◽  
pp. 67-71
Author(s):  
Jaime Pérez-Griera ◽  
Juan Carlos Andreu-Ballester ◽  
Alejandro Hueso Zarandieta ◽  
José García de la Asunción ◽  
Silvia Masquefa Bondia
Keyword(s):  
Immunosorbent Assay ◽  
Immunoglobulin A ◽  
Enzyme Linked Immunosorbent Assay ◽  
Secretory Immunoglobulin A ◽  
Secretory Immunoglobulin

Induced Polyspecificity of Human Secretory Immunoglobulin A Antibodies: Is It Possible to Improve Their Ability to Bind Pathogens?

Pharmacology ◽  
2021 ◽  
pp. 1-10
Author(s):  
Ekaterina N. Gorshkova ◽  
Shina Pashova ◽  
Ekaterina A. Vasilenko ◽  
Tatiana S. Tchurina ◽  
Elizaveta A. Razzorenova ◽  
...  
Keyword(s):  
Immunoglobulin A ◽  
Secretory Iga ◽  
Enzyme Linked Immunosorbent Assay ◽  
Antigen Binding ◽  
Secretory Immunoglobulin A ◽  
Acidic Ph ◽  
Ferrous Ions ◽  
Binding Properties ◽  
Binding Behavior ◽  
Secretory Immunoglobulin

<b><i>Introduction:</i></b> As has been shown previously, various protein-modifying agents can change the antigen-binding properties of immunoglobulins. However, induced polyspecificity of human secretory immunoglobulin A (sIgA) has not been previously characterized in detail. <b><i>Methods:</i></b> In the present study, human secretory immunoglobulin A (IgA) was exposed to buffers with acidic pH, to free heme, or to pro-oxidative ferrous ions, and the antigen-binding behavior of the native and modified IgA to viral and bacterial antigens was compared using Western blotting and enzyme-linked immunosorbent assay. The ability of these agents to modulate the antigen-binding properties of human sIgA toward a wide range of pathogen peptides was investigated using an epitope microarray. <b><i>Results:</i></b> We have shown that acidic pH, heme, and pro-oxidative ferrous ions influenced the binding of secretory IgA in opposite directions (either increasing or decreasing); however, the strongest effect was observed when using buffers with low pH. This fraction had the highest number of affected reactivities; most of them were increased and most of the new ones were toward common pathogens. <b><i>Conclusions:</i></b> Thus, it was shown that all investigated treatments can alter to some degree the antigen-binding of secretory IgA, but acidic pH has the most potentially beneficial effect by increasing binding to a largest number of common pathogens’ antigens.

scholarly journals Analysis of Occupational Stress and Its Relationship with Secretory Immunoglobulin A in the Xinjiang Plateau Young Military Recruits

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Ning Tao ◽  
Hengqing An ◽  
Jianjiang Zhang ◽  
Yuanyue Zhang ◽  
Lu Jin ◽  
...  
Keyword(s):  
Occupational Stress ◽  
Military Training ◽  
Immunoglobulin A ◽  
Demographic Characteristics ◽  
Enzyme Linked Immunosorbent Assay ◽  
Secretory Immunoglobulin A ◽  
Stress Group ◽  
Coping Ability ◽  
Military Recruits ◽  
Secretory Immunoglobulin

Background. With the continuous improvement of the modernization of the Chinese military and the major adjustments made by the state to the recruitment policy, the newly recruited military undergone multiple pressures such as targeted high-intensity military training and environmental changes. The mental health of military has become a crucial factor of improving the fighting capacity effectiveness of the troops. Objectives. To explore occupational stress of young recruits in the Xinjiang plateau environment during their basic military training period and analyze the relationship between occupational stress and secretory immunoglobulin A (sIgA) levels. Methods. Using multistage stratified cluster random sampling, 625 recruits stationed at Xinjiang plateau command in 2014 were enrolled as subjects. Occupational stress was assessed by the Occupational Stress Inventory Revised Edition (OSI-R). sIgA in saliva was quantified by enzyme-linked immunosorbent assay. The resulting data were analyzed using descriptive statistics, nonparametric tests, and correlation analysis. Results. Based on demographic characteristics, occupational stress was higher in the urban group than the rural group, coping ability for stress was greater in individuals who were students before joining the army than nonstudents, occupational stress was higher in smokers than nonsmokers, and coping ability for stress was higher in nonsmokers than in smokers (all P<0.05). Being an only child, educational level and age were not significantly related to occupational stress scores (P>0.05). Salivary sIgA level was higher in the high occupational stress group than in the low stress group (P<0.01). Salivary sIgA was positively correlated with scores on the occupational role and personal strain questionnaires (rs=0.229, rs=0.268, P<0.01). Conclusion. Demographic characteristics influenced occupational stress among young recruits in cold and high-altitude area. Further, there were some relationships between occupational stress and salivary sIgA in young military recruits.

scholarly journals The sIgA and histatin-5 expressions in saliva of early childhood caries and caries-free children based on period times

2021 ◽  
Vol 5 (1) ◽  
pp. 1-6
Author(s):  
Dharli Syafriza ◽  
Heriandi Sutadi ◽  
Ameta Primasari ◽  
Yahwardiyah Siregar
Keyword(s):  
Early Childhood ◽  
Early Childhood Caries ◽  
Immunoglobulin A ◽  
Enzyme Linked Immunosorbent Assay ◽  
Secretory Immunoglobulin A ◽  
Weak Correlation ◽  
Histatin 5 ◽  
Significant Difference ◽  
Secretory Immunoglobulin ◽  
Childhood Caries

To analyze the level of sIgA and Histatin-5 expression in Early Childhood Caries (ECC) and caries-free children. Saliva was collected from 31 subjects of ECC and 29 caries-free (ages 9-32 months). The level expression of sIgA and Histatin 5 was analyzed by enzyme-linked immunosorbent assay. The distribution and frequency of subjects consist of boys 47% and girls 53% with the average of ages 1-12 month 21% and 13-24 month 79%.sIgA and Histatin 5 expressions differ from the month periods tested (1, 3, and 9 months) but do not show any significant difference from each examination period (1 with three months; p0.05: 0.116), (1 with nine months; p 0.05: 0.766), and (3 to 9 months; p0.05: 0.398). The expression of sIgA and Histatin 5 of boys and girls were not significant (p0.05:0.550) and weak correlation (r = 0.079).The level expression of sIgA in the Caries-free higher than ECC (p0.05). Whereas the expression of Histatin 5 in ECC higher than Caries-free (p0.05). The sIgA correlating in ECC and caries-free is significant (p0.05) and no correlation (r =- 0,29). Whereas Histatin 5 in the ECC and caries-free were strong correlation = 0,84. Both the result of ECC and caries-free suggested that the antibodies have been involved in the caries pathogenesis. In the ECC cases that occurred to decrease sIgA levels and increased of Histatin 5 levels, both antibodies have expressed in Caries-free Children. Both sIgA and Histatin 5 involved in the pathogenesis caries infection in children.KEYWORDS: Antibodies; Early Childhood Caries; saliva; secretory immunoglobulin A; Histatin-5

scholarly journals Study of Humoral Immunity to Commensal Oral Bacteria in Human Infants Demonstrates the Presence of Secretory Immunoglobulin A Antibodies Reactive with Actinomyces naeslundii Genospecies 1 and 2 Ribotypes

2004 ◽  
Vol 11 (3) ◽  
pp. 473-482 ◽  
Author(s):  
Michael F. Cole ◽  
Mishell K. Evans ◽  
Jennifer L. Kirchherr ◽  
Michael J. Sheridan ◽  
G. H. W. Bowden
Keyword(s):  
Cell Wall ◽  
Immunoglobulin A ◽  
Oral Bacteria ◽  
Enzyme Linked Immunosorbent Assay ◽  
Secretory Immunoglobulin A ◽  
Cell Wall Proteins ◽  
Human Infants ◽  
Specific Antibodies ◽  
Actinomyces Naeslundii ◽  
Secretory Immunoglobulin

ABSTRACT The mouths of three human infants were examined from birth to age 2 years to detect colonization of Actinomyces naeslundii genospecies 1 and 2. These bacteria did not colonize until after tooth eruption. The diversity of posteruption isolates was determined by ribotyping. Using immunoblotting and enzyme-linked immunosorbent assay, we determined the reactivity of secretory immunoglobulin A (SIgA) antibodies in saliva samples collected from each infant before and after colonization against cell wall proteins from their own A. naeslundii strains and carbohydrates from standard A. naeslundii genospecies 1 and 2 strains. A. naeslundii genospecies 1 and 2 carbohydrate-reactive SIgA antibodies were not detected in any saliva sample. However, SIgA antibodies reactive with cell wall proteins were present in saliva before these bacteria colonized the mouth. These antibodies could be almost completely removed by absorption with A. odontolyticus, a species known to colonize the human mouth shortly after birth. However, after colonization by A. naeslundii genospecies 1 and 2, specific antibodies were induced that could not be removed by absorption with A. odontolyticus. Cluster analysis of the patterns of reactivity of postcolonization salivary antibodies from each infant with antigens from their own strains showed that not only could these antibodies discriminate among strains but antibodies in saliva samples collected at different times showed different reactivity patterns. Overall, these data suggest that, although much of the salivary SIgA antibodies reactive with A. naeslundii genospecies 1 and 2 are directed against genus-specific or more broadly cross-reactive antigens, species, genospecies, and possibly strain-specific antibodies are induced in response to colonization.

scholarly journals Regulation Effect of a Chinese Herbal Formula on Flora and Mucosal Immune Secretory Immunoglobulin A in Rats

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Tian-hao Liu ◽  
Xiao-mei Zhang ◽  
Ni-ping Han ◽  
Yang Liu ◽  
Yue-ying Wu ◽  
...  
Keyword(s):  
Immunoglobulin A ◽  
The Body ◽  
Enzyme Linked Immunosorbent Assay ◽  
Secretory Immunoglobulin A ◽  
Herbal Formula ◽  
Model Group ◽  
Chinese Herbal Formula ◽  
Chinese Herbal ◽  
Rdna Sequencing ◽  
Secretory Immunoglobulin

Flora and mucosal immunity are considered to be the barrier, which is associated with multiple respiratory diseases, including recurrent respiratory tract infection (RRTI). Fei-Xi-Tiao-Zhi-Fang (FTF) is a traditional Chinese herbal formula used in the treatment of RRTI. However, the mechanism is little known. This study aims to identify the function of FTF in flora and mucosal immune secretory immunoglobulin A (sIgA) in the model of RRTI rats. The samples of intestine and lung were collected to detect sIgA, short chain fatty acids (SCFAS), and flora with enzyme-linked immunosorbent assay (ELISA), gas chromatography, and 16S rDNA sequencing. The body weight and viscera index were increased dynamically in RRTI rats after the administration of FTF. Furthermore, the types and proportions of aboriginal flora were significantly changed in the model group, whereas the altered flora was rescued in the FTF administration group. Desulfovibrio increased in the intestinal microflora and Ralstonia and Blautia decreased in the pulmonary microflora at the genus level, similar to that in the normal group. In addition, the expressions of sIgA in pulmonary and intestinal tissues were significantly upregulated and the level of SCFAS was increased in FTF group compared to the RRTI model group. Our study suggests that FTF can alleviate the symptoms of RRTI by increasing sIgA and SCFAS, recovering flora, and improving the immunity.

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